We narrowed to 1,024 results for: PLE

...listen for hints at the same concerns from multiple people. Listen to what they say, and what they don...grad school vs. postdoc Choosing an advisor to complete a successful PhD is different from choosing a ...confidentiality to ensure more honest answers. Ask people who work in the labs next door for their opinions...

...genome engineering tool offers flexibility, multiplexibility, and ease of use. In order for this technology...protospacer adjacent motif (PAM). Once the CRISPR/Cas complex binds to the foreign DNA, a cut is made to destroy...the tool’s specificity, orthogonality, and multiplexibility in various species and describe an assortment...from experts in the field, and enabling access to multiple plasmids used for various platform applications...

... sequencing and RNA sequencing of complex heterogeneous RNA samples. Furthermore, such high processivity...ever like to write about a recent plasmid deposit please sign up here. Listen to the Hot Plasmids Podcast...technologies based on polymerase processivity. Please find the MarathonRT plasmid here! Zhao C, et al...

...Collaborations may be less “complicated” - for example, easy to initiate a collaboration without having...matrix - can be frustrating for solo flyers Other people will routinely scrutinize your data—science can...issues can create interpersonal issues (one bad apple can change entire culture) Specific requirements...Can move between the two (helps to know the right people) -> How can you make a decision about something...

...method kills the transgene free individual. For example, with CRISPR-Cas9 gene editing, transformation ...GUS staining or are not suited to studying large samples. RUBY-Red But recently, something new has been ...belongs to a different chemical family than the red/purple plant pigments found in all other plants, anthocyanins...Other innovative uses of RUBY are possible, for example as positive selection of transformed plants to ...

... Biosensor GENIE Project New viral prep with multiple serotypes pAAV-hSyn-GRAB_OTmut AAV1 Biosensor...Biosensor Robert Campbell New viral prep with multiple serotypes AAV-hSyn-Ribo-jGCaMP8s AAV1 Biosensor... AAVrg Control Karl Deisseroth New serotype (multiple) rAAV-CAG::FLEX-rev:: hM4D-2a-GFP AAV1 Chemogenetics... Multiple Authors...

...The triple T’s of biology are transformations, transfections, and transductions! In this blog we will...expressed. Once the design and construction steps are complete, it’s time to make virus! The constructed viral...the biological outcome you want to achieve? For example, do you want the nucleic acid cargo to be integrated... to achieve different biological outcomes. For example, some viruses integrate into the host genome (lentivirus...

...interact during various behaviors. Either tissue samples were harvested after the behavior, or the animals...adaptable tool that could continuously monitor multiple systems. Ideally this tool would be wireless, ...develop these fibers, the labs looked at previous implementation of optogenetic tools, calcium sensors, and ...optical stimulation of its associated fibers across multiple channels and can also record tissue temperature...

...Viruses are simple: a genome packaged in a protein shell (Taylor, 2014). They’re so simple that we can... even decide if they’re alive or not. Yet these simple, small particles have quite the outsized impact... been genetically modified to either limit or completely eliminate their replicative ability. The remaining...

...modular, selection-based gene tagging approach Multiple features of C. elegans have made CRISPR/Cas9 so...HDR mentioned above, an accessible germline, and multiple selectable markers. Bob Goldstein’s lab previously...The data are clear - the SEC-based system is both simple to use and highly efficient for C. elegans genome...elegans protein. The his-72 locus is shown as an example. Used with permission from the Genetics Society...

..., in turn, the big picture of the project. For example, how can future regulations impact your work? What...word “extracurricular” may be taboo in the lab, multiple research studies highlight the importance of taking...potential careers to gather more information Follow people in academia, industry, and government on social...sounds interesting, TAKE ACTION! Taking action: Examples of extracurricular activities The table below...

...cellular level. The reason for this is cost and complexity – anyone who works in the lab knows how expensive... engaging for students. In our BioBits kit for example, we’ve designed FD-CF experiments that result in...educational activities using the BioBits platform, please get in touch with us! We’ll work with you to get...Addgene.org Browse our protocol videos to learn simple lab techniques Check out our molecular biology ...

...practical and hands-on. Students in these courses get plenty of theory in their lectures, but it can be difficult... format I decided on was to have two teams of 4 people face each other on a single chosen clinical disease...modify BUILD YOUR PLASMID or generate games like it, please freely share your projects with us! This exchange...want to receive our modifiable powerpoint file, please feel free to contact us at: [email protected]...

...ul) samples of EGFP-expressing AAV packaged in various serotypes. This collection is now complete! pAAV-CAG-GFP.... Likewise, a lot of scientists have requested samples of AAV for testing purposes. To address this, we... kit with all the serotypes, since these 20 ul samples are distributed individually. See our Serotype ...

...you can fully investigate and solve a problem or complete a project, overcoming inevitable failures and ...Technologist/Designer     Fieldwork and traveling Many people are attracted to science because they enjoy spending...shows that you are passionate and capable. For example if the job involves traveling, showing that you...organised and experienced traveler by citing specific examples, may help you get your foot in the door. Match...

...accelerating research into this virus. One such example is cell lines. The American Type Culture Collection...global endeavor. Case study 2: Plasmids Another example of reagents that have broad applications but have...dozens of times in 2 weeks. This is a striking example of the contributions that scientists are able to...popularity of the hACE2 transgenic mouse strain, for example, is sustained by the availability of this strain...

...major histocompatibility complexes (MHC class I and II).  There are multiple algorithms, both open-access... antibody drugs are popular therapeutics for a plethora of disease conditions, from cancer to autoimmune...approach The in silico approach cannot replicate the complexity of antigen processing events that take place ...

...JA. Precise transcript targeting by CRISPR-Csm complexes. Nat Biotechnol. 2023 Jan 23. doi: 10.1038/s41587...only having one binding site per protein. Having multiple antibodies allows you to use them in parallel ...recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain. Elife. 2019 Jan 22;8:...  Multiple Authors...

...emotional time!” George Church tries to recruit people who are nice as top criterion and offers advice...productive in a handsoff environment. “Treating people fairly does not mean equally.” George Church was...comments that it is also surprising “ how easily people talk themselves out of great projects as ‘impossible...building up your lab and selectively picking the first couple of hires to build a strong, collaborative team....

...for plasmid replication in mammalian cells, for example, and a toxin that kills bacteria may not have any... is possible that you may not want the plasmid depleted, but still want to use transient transfection ...HeLa, NIH3T3, CHO, COS-1, 293HEK, Jurkat T cells Complexes with DNA; causes strand scissions 100-400 ug/mL...your cells divide. Gentamicin is often used as a supplement in mammalian cell culture to suppress bacterial...