Tips for Using FRET in Your Experiments
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Blog Post
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Zimmermann T, Terjung S. FRET Basics and Applications: An EAMNET Teaching Module http://www.embl.de...
We narrowed to 663 results for: PPL;
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Data Freedom: The Expansion of Data Sharing in Research Publications
TypeBlog Post...distinct (perhaps similarly impoverished lab) might apply different or novel tools and assumptions to reveal... -
Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus
TypeBlog Post...and may need to be optimized for each unique application (Trapani et al., 2015). Expression of smaller... -
The Fluorescent Vegetables in Aptamer Soup
TypeBlog Post...aptamer soup by exploring the development and applications of fluorescent RNA aptamers in Addgene’s collection... -
CRISPR 101: Validating Your Genome Edit
TypeBlog Post...sequencing confirmation. All knock-ins A method applicable to knock-ins both large and small is a software... -
Fluorescent Proteins 101: When GFP lets you down
TypeBlog Post... proteins.Therefore, GFP is not suitable for applications that require anaerobic conditions. In experiments... -
10 Steps to a Perfect Science Talk
TypeBlog Post...non-academic settings. Many of these guidelines will apply to any type of science presentation, but, for more... -
Site Directed Mutagenesis by PCR
TypeBlog Post...of CRISPR; site-directed mutagenesis generally applies to plasmids, but may also facilitate genome editing... -
Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?
TypeBlog Post...lessons learned in pursuing one technological application can benefit many others and accelerate the pace... -
Antibodies 101: Introduction to Gating in Flow Cytometry
TypeBlog Post...voltage describes an electric potential that can be applied to the photomultiplier inside the cytometer to ... -
An Integrin Antibody Toolkit from IPI
TypeBlog Post... in flow cytometry. Based on the established applications of the original hybridoma monoclonals, we anticipate... -
How to Negotiate a Successful Lab Start Up Budget
TypeBlog Post...discounts, collaborations, access to internal award applications, etc. If this is the institute that can help... -
CRISPR 101: Epigenetics and Editing the Epigenome
TypeBlog Post...to specific DNA sequences. Non-editing CRISPR applications direct catalytically dead dCas9 fused to a variety... -
Hot Plasmids: Spring 2025
TypeBlog Post...popular choice for a variety of synaptic imaging applications. Find iGluSnFR4 plasmids here! Aggarwal, A.... -
Plasmids 101: Restriction Cloning
TypeBlog Post...toward the cathode (- charge). When the voltage is applied across the gel, the DNA migrates toward the anode... -
A History of Genome Engineering in Popular Culture
TypeBlog Post...extracting the DNA from fossilized amber and supplementing it with the DNA of present-day frogs via genetic... -
A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression
TypeBlog Post...using. Contact Addgene ([email protected]), or the supplier of your vector, to obtain this information. Optimize... -
Quick Guide to All Things Lentivirus
TypeBlog Post...have been increasingly popular in fundamental and applied research since their first use in the early 90’... -
Deep Dive: Statistical Tests (Comparisons)
TypeBlog Post...Variables: A Monte Carlo Simulation Study. Front. Appl. Math. Stat. Volume 2 (2016). doi.org/10.3389/fams... -
Antibodies 101: Flow Cytometry
TypeBlog Post...protein composition. It is used for a variety of applications such as measuring target protein expression ...