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We narrowed to 1,006 results for: RING

Showing: 621 - 640 of 1006 results

  1. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    ...mass bleaching, the loss of their symbiotic algae, during heat stress. For other organisms, like the sea ... Wistrand-Yuen, P., … Forster, A. C. (2018). Engineering a palette of eukaryotic chromoproteins for bacterial...bacterial synthetic biology. Journal of Biological Engineering, 12(1). https://doi.org/10.1186/s13036-018-0100...

  2. Academic vs. Industry Postdocs

    Type
    Blog Post
    ...an industry postdoc is something that you’re considering. Like anything, there are pros and cons to postdocs...number of steps, decisions and people involved is staggering. The process is complicated. If you’re interested...all about teamwork. A single individual could not bring a drug to the market, so people in industry have...

  3. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    ...more gRNAs must be used along the same chromosome. During DNA repair, there is a chance that the two breakpoints...Harnessing type I CRISPR–Cas systems for genome engineering in human cells. Nature Biotechnology, 37(12),...CRISPR-Cas Systems INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons Additional resources...

  4. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ...powerful tool, it was promising enough to keep exploring and to try developing a variant with an enhanced...variants of MagLOV will be possible with other engineering approaches and that magnetosensitive tools can...often posting updates on social media) and freely sharing code, raw data, and more. The team shares results...

  5. Pooled Library Amplifications

    Type
    Blog Post
    ... library is maintained and reduce recombination during the amplification process. In liquid culture, all...recombination. If you do see recombination, we recommend ensuring the cells used are meant for repetitive sequences...Addgene blog Genome-Wide Screening Using CRISPR Preparing Pooled Libraries NGS Quality Control for Pooled...

  6. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

    Type
    Blog Post
    ... unable to participate in genetic recombination during meiosis. Also, flies are not viable if they are...know is that males do not recombine chromosomes during meiosis. This can sometimes be a handy feature ...Like Addgene, the Drosophila community is highly sharing-oriented, and there are many resources to help ...

  7. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    ...separate proteins should be useful for monitoring cellular events occurring at varied time scales.  Table 1:...the FP timers, add an entire new dimension to monitoring localization; using FP timers, researchers can...

  8. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    ...media with supplements such as magnesium salts, buffering agents, and/or supplying additional carbon sources... yield. Start with a single, fresh colony Sub-culturing directly from a frozen glycerol stock or agar ...harvesting and miniprepping at various time points. Measuring the optical density of the culture can indicate...

  9. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    ...blastn program uses a smaller word size and lower scoring penalties for mismatches and gaps in the alignment...blastp algorithm parameters. We use the default scoring matrix BLOSUM62, but you may want to check the ... multiple protein sequences, particularly for comparing different isoforms. In addition to our favorites...

  10. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

    Type
    Blog Post
    ...for distribution. If your experiment involves delivering a calcium biosensor using AAV, you might decide...viral preps instead of plasmids? The available filtering options now change dynamically based on the type...target organism. Clicking through to RNA Editing brings us to a page with a basic overview and figure, ...

  11. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ... It may be helpful to incorporate regular feeds during this incubation time to maintain cell viability...consider increasing the number of washes performed during your harvest. Validation Recombinant antibodies...temperature shifts. Of course, we are also cautious during the aliquoting and storage process to prevent dropped...

  12. An Introduction to Adenovirus

    Type
    Blog Post
    ...inhibition of the innate immune system; and reconfiguring of the nuclear compartment, gene expression,...’ functions are rescued by helper plasmids used during production and/or by genes present in the HEK293T...license.   The promise of adenoviral vectors This brings us to the final topic, which is that natural AdVs...

  13. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    ... the genome, they could promote oncogenesis by altering local gene expression. Self-inactivating (SIN)... PubMed Central PMCID: PMC4486245. 5. Patient monitoring and follow-up in lentiviral clinical trials. ... Mary Gearing...

  14. Plasmids 101: Colony PCR

    Type
    Blog Post
    ...up colony PCR reactions is nearly identical to preparing a standard PCR reaction: combine template, primers...other colony PCR tips are highlighted below. Preparing template: Pick a single colony with a sterile ...to the PCR reaction. The bacteria will be lysed during the initial heating step of the PCR reaction. A...

  15. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    ...requirements. Cpf1’s preferred PAM is 5’-TTN, differing from that of Cas9 (3’-NGG) in both genomic location...repair templates should remove the PAM sequence. Comparing Cpf1 and Cas9 on-target and off-target efficiency... Mary Gearing...

  16. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    ... training, mentoring and coaching services, including Smartegrants, to help those entering the Grants ...Twitter Chat, and then our grant training and mentoring. How do I become a grant professional? Employment...

  17. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ...first non-Cas9 CRISPR protein adapted for genome engineering was Cas12a (formerly Cpf1), a nuclease that generates...comment below. Note: Jennifer Tsang and Mary Gearing contributed to the update of this post. References... genome engineering...

  18. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ... the promiscuous cleavage and degradation of neighboring ssRNA and ssDNA by Cas13 and Cas12a, respectively....org/10.1038/s41587-020-0513-4 Chen JS, Ma E, Harrington LB, Da Costa M, Tian X, Palefsky JM, Doudna JA...Essletzbichler P, Dy AJ, Joung J, Verdine V, Donghia N, Daringer NM, Freije CA, Myhrvold C, Bhattacharyya RP, Livny...

  19. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ...proteins (FP) have also been important in mapping, monitoring, and manipulating neuronal circuits. Epitope ...tags, low FP expression may be insufficient for monitoring a weakly expressed POI. Spaghetti Monster - A...fluorescent protein structure and color variants." Cold Spring Harbor Protocols 2009.12 (2009): pdb-top63. PubMed...

  20. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    ...subpopulations of cells, you’ll soon find yourself encountering flow data in the literature. Data reported from...of flow cytometry is to use increasingly more stringent markers to identify your specific cell population...are used to separate single cells from doublets, ignoring anything not within the gate of the first plot...
Showing: 621 - 640 of 1006 results