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  1. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    ...Once upon a time, not so long ago, spCas9 was the only Cas enzyme widely available and applied by researchers...better experimental fit due to this. But now, it’s time to cast aside those concerns and embrace a new resource...

  2. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    ...Will it be sensitive enough? No one wants to spend time screening hits that aren’t “real” or novel for their... understand the difference between the two, it’s time to check out our CRISPR library resources or our...

  5. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    ... of Cas9 expression between experiments and over time (as compared to separate transductions with a single-vector...(Gi)-mCherry When using this virus for the first time, the Roth Lab recommends verifying cre expression...

  6. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    ... folder that you are currently working in) every time you use them, leave them in their own folder. Doing...Organized Learn about Software You Can Use to Save Time and Track Your Experiments Try out the CRISPR Software...

  8. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    ... and develop skill in science communication over time.  Like most skills, great science communication ... to pose these why questions to yourself, but ultimately there is no substitute for feedback from others...

  11. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    ...acid sequence of the antibody-encoding genes over time as the hybridoma cells divide. These changes can...meaning that there will be changes between lots over time. Specifically, the antigen-binding sites of the ...

  12. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    ...software tools for the right people at the right time. To explain a bit further, a software product manager...going to need to be a problem-solver. A lot of the time, the problem to solve is clear, but the solution...

  13. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    ...targeting. While promoters and AAV serotypes can sometimes be used to target tissue types, recombinases allow...next 6 months, pending quality control. For an estimate on availability date or to be notified when a ...

  15. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    ...catalog are very well-characterized. During this time, we’ve also created a variety of educational resources...the foundations for many improvements to come. Ultimately, we would like our search engine to be so specialized...

  16. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    ...a talk about the different parts of the brain stimulated by stories. In retrospect, I should have expected...looking to get into the field, this is a fantastic time to do it! Additional Resources on the Addgene Blog...

  17. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    ...process: It is important to give your plates enough time for any intact β-galactosidase to be expressed and...is evenly distributed and allow sufficient drying time before use. Beware of false positives: Blue-white...

  18. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    ...In the short time since its development, CRISPR/Cas9 genome editing has been used to study the effect...transcript levels of PTG1 and PTG2 were 3 and 31 times higher, respectively. In rice protoplasts, Xie et...

  19. To Each HIS Own

    Type
    Blog Post
    ...the activity or function of a single protein? Oftentimes, they rely on protein purification. In this strategy...to the metal ions once the protein is folded. Sometimes a tag placed on one end will be blocked during...

  20. All in a Twist: dsRNA

    Type
    Blog Post
    ...have been harnessing the power of dsRNA for a long time, even before we knew about all the diverse in vitro...Endogenous sources of duplexed RNA DNA:RNA hybrids Sometimes ssRNA just needs a buddy, and even DNA will suffice...
Showing: 621 - 640 of 925 results