We narrowed to 911 results for: TRO

...base pairing between these ~4 base overhangs isn’t strong enough to hold a plasmid together through transformation... reading Harnessing homologous recombination in vitro to generate recombinant DNA via SLIC. Li MZ, Elledge...

..., you’ll want the proper positive and negative controls for your PCR.  Find the details and learn more... this blog post for tips for analyzing and troubleshooting Sanger sequencing results. At Addgene, we now...

... Image from Wen et al., 2017. Once you’ve introduced the reporter into the cells, you’re ready to ...sequence is also found in the reporter gene). A control RFP expression plasmid.  With this set up, all...

...Ginkgo Bioworks COVID-19 Plasmid Collection Quality control at Addgene in the midst of COVID-19 We received...meant that we had to revamp some of our quality control processes. For the many other COVID-19 related ...

...employing a variety of cloning techniques and troubleshooting methodologies drew me to learn more about the...: Secondary Nanobody Toolbox Antibodies 101: Introduction to Antibodies Resources on Addgene.org  Browse...

...Please keep in mind that this is just a brief introduction to the wide, wild, and fascinating field of ...Antibodies 101: Polyclonal Antibodies Antibodies 101: Introduction to Antibodies Resources on Addgene.org ...

...improved RNAi vector that leads to significantly stronger phenotypes than standard RNAi protocols without...domain family, known to be involved in cytoskeletal control, cell growth, and endocytosis. Recently, the Sidhu...

...operon.   Tips for blue-white screening Use a good control: Transform the backbone plasmid without insert....reproduce to form colonies (used to complement auxotrophic cell lines), or genes which rescue a lethal phenotype...

...other than to sustain those techniques. We feel strongly that the scientific and the agricultural community...aesthetics with this technology and giving consumers control over their interaction with it, we hope to inspire...

...successfully labeled protein-coding genes with both intronic and exonic gRNAs. In fact, the method is specific...of the Pitfalls When Using Fluorescent Proteins Control Protein Activity with GFP Resources on Asddgene.org...

...the hands of our finance team, now 3.5 Addgenies strong. The Addgene Board of Directors is mature and...and I believe they know and trust this. We’ve introduced cutting edge practices and benefits and these...

... means that F(ab’)2 fragments may have overall stronger interaction with their antigens than a Fab or ....2022.100301  Additional resources on the Addgene blog: Introduction to AntibodiesSingle Chain Fragment Variables...

...the lab (for a refresher see Antibodies 101: Introduction to Antibodies.) Unfortunately, for some proteins...Protein Tags Antibodies 101: ChIP Antibodies 101: Introduction to Antibodies References Hochuli E, Bannwarth...

..., Wang, Hou, Zhonggang, Xiao, Yibei, et. al. Introducing a Spectrum of Long-Range Genomic Deletions in...  Susanna Stroik...

...of the two fragments. However, rigidity can be introduced through mutation, allowing diabodies to be used...FluorophoresAntibodies 101: scFVs Antibodies 101: Introduction to Antibodies Additional resources on addgene.org...

...for cellular delivery of RNAs By Mike Lacy The retroviral-like protein PEG10 specifically binds its own...small insertions to allow in-frame deletions and introduce epitope tags and RNA transcription start sites...

...degraded. To effectively isolate intact RNA, a fast, strong protein denaturant would be required- something...late 1970’s, Chirgwin and colleagues showed that a strong protein denaturant, guanidinium thiocyanate, did...

...lox system with optogenetics. Cre activity is controlled by red light by splitting Cre and fusing each...system, cells express various fluorescent proteins controlled by Cre recombination. Each cell can have multiple...

...triggering new cellular behavior like degradation or electrophile signaling. Many of these tools can be found ...References Devaraj, N. K., & Finn, M. G. (2021). Introduction: Click Chemistry. Chemical Reviews, 121(12),...

...phenotype in a scalable way. This blog post will introduce you to this new technology, and guide you through... Luke A., et al. "Genome-scale CRISPR-mediated control of gene repression and activation." Cell 159.3 ...