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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 746 results for: UTY

Showing: 621 - 640 of 746 results

  7. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    ...effector nucleases. These plasmids were designed to utilize the BsaI and BsmBI Type IIS sites such that custom...

  9. Immunocytochemistry

    Type
    Protocol
    ...working solution: Prepare a 300 µM DAPI stock solution by diluting 2.1 µL of the 5 mg/mL DAPI solution to ... 300 nM DAPI working solution by diluting 5 µL of the 300 µM DAPI stock solution into 5 mL PBS. Protect... and 30 µL Triton X-100 in 10 mL PBS. Antibody dilution buffer: Dilute 0.5 g BSA and 150 µL Triton X-100...Remove the paraformaldehyde and follow your institution's laboratory safety guidelines for disposing of...antibody to the desired concentration in antibody dilution buffer. Pro-Tip The optimal antibody concentration...Counterstain nuclei with 500 µL of 300 nM DAPI working solution for 10 min at RT in the dark. Remove the DAPI ...

  10. AAV Production in HEK293 Cells

    Type
    Protocol
    ...hydrochloric acid until the solution clears. Check the pH of the solution and use hydrochloric acid or... 1 g/L glucose. 1 mg/mL polyethylenimine (PEI) solution: Dissolve 100 mg of PEI powder into 100 mL of ...to adjust the pH to 7.0. Pro-Tip The pH of this solution will drift pretty rapidly upon addition of acid...over or undershooting the desired pH. Allow the solution to mix for 10 min and then recheck the pH to ensure... ensure that it has not drifted. Filter the solution through a 0.22 μm membrane. Aliquot 500–1000 μL into... Store the tubes at -80 °C. After thawing, the solution can be stored at 4 °C for up to 2 months. After...working stock. 40% POLYETHYLENE GLYCOL (PEG) 8000 solution: Dissolve 400 g of PEG 8000 and 24 g of NaCl ...

  11. Fluorescence Titering Assay

    Type
    Protocol
    ...will be higher titer, consider additional dilutions. Dilution Volume of Lentivirus Stock (μL) Volume of... Mix the dilutions well Gently aspirate media from the cells. Add 1.5 mL of a viral dilution to each well...multiple dilutions. Sample Data Figure 1: 293T cells were transduced with a range of dilutions of pHAGE-TO-dCas9...ensure that you are in compliance with your institution’s biosafety regulations. Workflow Timeline Day...lentiviral aliquot on ice prior to use. Prepare dilutions of the lentivirus into DMEM complete containing... well (each well gets one dilution with one well remaining as the untransduced control). Count the cells...transduction units per mL (TU/mL) using either the dilution factors (method 1) or the volume of virus (method...

  12. Design Tips for Prime Editing

    Type
    Blog Post
    ... newly synthesized strand before heteroduplex resolution, which can lead to indels (Anzalone et al., 2019...

  15. Rabies and Neuronal Tracing

    Type
    Blog Post
    ...Retrograde monosynaptic tracing reveals the temporal evolution of inputs onto new neurons in the adult dentate...

  19. Tips for Technical Support Calls

    Type
    Blog Post
    ...interaction - one that gives our customers the most utility in the least amount of time. Though I now spend...
Showing: 621 - 640 of 746 results