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  1. Science as Art

    Type
    Blog Post
    ...Beautiful microscope images which traditionally languished in the pages of thick textbooks are now liberated...
  2. Virus Protocol - Generating Stable Cell Lines

    Type
    Protocol
    ...fresh media containing selection reagent Day 3–14: Change media as needed Day 14–18: Expand and harvest stable...cell line. To do this, treat target cells with a range of doses of antibiotic and determine the lowest ...lentiviral aliquot on ice prior to use. Prepare a range of dilutions of the lentivirus in DMEM complete ...titered your virus beforehand, you can narrow this range according to the results of your titration. Mix ...lentivirus, above) are dying. Perform regular media changes and monitor the growth of the cells. Pro-Tips Depending...culture, so it is important to do regular media changes and maintain optimal growth conditions for the ...absence of cell death, the cell media should be changed every 2–3 days to maintain the dose of antibiotic...
  3. SunTag and Fluorescent Imaging

    Type
    Blog Post
    ...localized, and how its localization and expression may change across various conditions. However, this system...
Showing: 621 - 640 of 827 results