We narrowed to 841 results for: ang
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TypeBlog Post... Jennifer Tsang...
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Editor's Choice, July 2016
TypeBlog Post...the entire blog and hosted new posts on topics ranging from new tools you can use to monitor cellular ... -
Addgene’s Blog Gets a New Look!
TypeBlog Post... Jennifer Tsang... -
The Developmental Studies Hybridoma Bank: Over 25 Years of Antibody Sharing
TypeBlog Post...the DSHB, play an important role in scientific exchange. In addition to ensuring affordable access to ... -
Make a Splash: Notions of Scientific Impact Are Evolving
TypeBlog Post...has increasingly moved online, surely times have changed. Altmetrics.org calls to expand our view of what... -
A Scientist's Guide to Ebola
TypeBlog Post...through the air and that’s considered unlikely to change – but the illness has been fatal in 70 percent ... -
Inntags: Innovative Protein Epitope Tagging
TypeBlog Post...with multiple other proteins. Again, little to no change in cell morphology was observed with IT5 and IT6... -
How Exercise Purges Stress and Keeps Depression at Bay
TypeBlog Post...brain is protected from stress-induced biochemical changes that could otherwise lead to depression. And there... -
Why Do I Need an MTA Anyway?
TypeBlog Post...this process. We take pride in facilitating the exchange of not just the physical samples, but also the... -
Quick, Versatile Plant Transgenesis with GreenGate Plasmids
TypeBlog Post...Because the fragments produced will have different overhangs, they can be designed such that they go back together... -
Reflections and Looking Ahead
TypeBlog Post...Content Accessibility Guidelines (WCAG). These changes mean our content is both available and accessible... -
Bringing Sustainable Practices to the Lab: Innovations
TypeBlog Post...variety of pipette tips. This is part of a cultural change in how we approach waste that needs to be supported... -
Top 10 Open Science Developments of 2013
TypeBlog Post... June, the White House honored 13 “Champions of Change,” including our recent guest Drew Endy, in celebration... -
Phage Directory: From Phage Therapy to a Repository of Phage Information
TypeBlog Post... Jennifer Tsang... -
SciComm with the Experts at Science in the News Part 2
TypeBlog Post...answer the question, will the effects of climate change make put Cambridge underwater? Chapter 8: Work-... -
Science as Art
TypeBlog Post...Beautiful microscope images which traditionally languished in the pages of thick textbooks are now liberated... -
CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]
TypeBlog Post...following paired DSBs is inversions [3]. These rearranged segments may also be informative as to genomic... -
Celebrating Accomplishments in the Lab
TypeBlog Post...those have been trips to breweries who happily exchange our expertise with lots of free beer.” He also... -
"What Makes a Good Mentor?" and 6 More FAQs About Science Mentoring
TypeBlog Post...thinking about how to be a great Mentor. Check Out Angela Depace's Talk on "Precision and Plasticity in Mentoring... -
Protocol - pLKO.1 – TRC Cloning Vector
TypeProtocol...sequences from step B.1 into the oligos below. Do not change the ends; these bases are important for cloning...CO 2 for 12-15 hours. Day 3: j. In the morning, change the media to remove the transfection reagent. Replace...Lentiviral particles can efficiently infect a broad range of cell types, including both dividing and non-dividing...to cells. Days 3+: e. Examine cells each day and change to fresh puromycin-containing media every other...Target cells should be approximately 70% confluent. Change to fresh culture media containing 8 μg/mL polybrene...particles per cell. Addgene recommends that you test a range of MOIs to determine the optimal MOI for infection...Incubate cells at 37°C, 5% CO 2 overnight. Day 3: e. Change to fresh media 24 hours after infection. TIP: If...