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...matrix (usually agarose) to pull your negatively charged DNA through the gel. As indicated in the figure...the gel in wells positioned toward the cathode (- charge). When the voltage is applied across the gel, the... the DNA migrates toward the anode (+ charge). Larger fragments of linearized DNA migrate slower than ...

...quantifies the likelihood that a result is due to chance vs. the factor that is being experimentally manipulated...) or skewed (see Fig. 2). Assumptions – characteristics of a data set; the ‘rules’ a statistical test...trial, for example).   Fig. 3: Question flowchart for choosing an ANOVA/MANOVA statistical test ...

... Figure 2. Schematic diagram showing the mechanism of spontaneous reversion during AAV plasmid amplification...phenotype in a mixed population (Burger, C. 2004, Aschauer, DF. 2013, O’Carroll, SJ. 2021). This is due to...outcome.    References and resources References Aschauer DF, Kreuz S, Rumpel S (2013) Analysis of Transduction...

...adapted from Zhou and Melton, 2008, [43]): Characteristic Reprogramming+ Transdifferentiation in vivo...oncogene is one of the reprogramming factors) More Chances of accumulation of harmful mutations More, due ...21434814. PubMed Central PMCID: PMC3161101. 20. Chang, C.J., et al., Production of embryonic and fetal-like...

...Antibodypedia Any Any Data repository   YCharOS Human proteins Immunoblot (western blot), IP...BioMolecular Atlas Program (HuBMAP)  Antibody Characterization Laboratory Cancer Any Data repository...

...template, CRISPR can be used to introduce a functional change in the DNA sequence. Base Editors: Modified versions...versions of CRISPR nucleases that make single base changes in the DNA sequence without completely cutting ...

...covered in this segment Hot Plasmid 1: CRISPR X from Michael Bassik's Lab - A new tool for making diverse, localized...this kit to complement unused auxotrophies in Saccharomyces strains.  Listen to Our Segment on The Yeast...

...(old board games and movies can provide great character costumes). All of this costume planning is for...guess each plasmid? Our lab paid tribute to the changing seasons – and they also invited Father Time to...

...created a microbe advent tree displaying over 25 archaea, bacteria, fungi, and viruses. Final tree with...MicrobeAdventCalendar ornament set (plus more!) It features archaea, bacteria, fungi, viruses, and one microbe ornament...

...dendritic spines!  Curious? Watch our AAV Data Hub Challenge recording to see two reports presented by the ...report describes a functional vector such as a channelrhodopsin Images showing the injection site as well ...

...generally listed as the amino acid change and not a nucleotide change. The proper way to denote an amino...

... Considering citations to all articles, Anurag Acharya and colleagues report, the percentage of citations...has increasingly moved online, surely times have changed. Altmetrics.org calls to expand our view of what...

...through the air and that’s considered unlikely to change – but the illness has been fatal in 70 percent ... Ebolavirus (EBOV) is a Filovirus, a family characterized by single, linear, negative-sense, single-stranded...

...2.2, and provide an indication of purity versus chaotropic agents as guanidine thiocyanate and guanidine...be entirely endotoxin free. Endotoxin is a lipopolysaccharide of the cell wall of Gram negative bacteria...

... Zoom’s default backgrounds all year and want a change? Addgene has you covered. Today we’re releasing...next meeting.     If you’re reading this blog, chances are you are a fan of Addgene or our very cute mascots...

...depression, and now researchers have reported a mechanism based on studies of transgenic mice that helps...brain is protected from stress-induced biochemical changes that could otherwise lead to depression. And there...

...must do science to be happy. What do we have to change to ensure that all scientists can have successful...training too many scientists and some propose radical changes to the way we view the graduate school and postdoc...

...Scientists at North Carolina State, in the labs of Chase L. Beisel and Rodolphe Barrangou, had applied the...Powerful New Way to Edit DNA" and "This Protein Could Change Biotech Forever."  David S. Weiss of Emory University...

...by scientists, which is hosted on our Instagram channel every Sunday. What's planned for the upcoming year...while “manipulate” could mean “worked with” or “changed” to scientists. Keep your message to the main three...

...many molecular biology methods such as allelic exchange, FRET, and synthetic biology. 7. 25 Work From...them in the cell. This blog post features two mechanisms that cause plasmids to be incompatible. 4. RNA...