We narrowed to 911 results for: ire;

1. Plasmids 101: Origin of Replication

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   ...varies. See below for additional considerations. *Requires pir gene for replication (reference). **F1 is...always pick a single colony and do not subculture directly from glycerol stocks, agar stabs, or liquid cultures...

2. Plasmid Cloning by PCR

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   ...: Do not cut within your insert Are in the desired location in your recipient plasmid (usually in ...amplified (the ORF in this case), not the Tm of the entire primer. If you are amplifying from a plasmid or...

3. Multiplexed Overexpression of Regulatory Factors (MORF) collection

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   ... presence influences cell fates. The atlas of directed differentiation To understand how each human ...Joung J. et al., A transcription factor atlas of directed differentiation. Cell. 2023. 186 (1) 209-229. ...

4. Using CRISPR/Cas9 to Edit Disease Out of the Genome

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   ...Berkeley’s Jennifer Doudna. “They are the first direct demonstration of use of genome editing to correct...need to get a handle on how cleavage sites are repaired.” Those insights will be critical for scientists...

5. Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

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   ... research projects to a halt. None of us works directly with viruses or transmissible diseases, so we ...and availability; 3) Make the contact to start a direct collaboration between researcher and expert; 4)...

6. Reagent Repositories Are Speeding up Science During the Pandemic

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   ...Scientists need fast access to the tools required to study SARS-CoV-2, but with many academic research...Addgene.org Find COVID-19 plasmids at Addgene Browse the entire plasmid collection ...

7. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

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   ...ml This method is quick and simple and doesn’t require any special reagents. However, there’s limited ...absorbance-based methods, fluorescence-based methods require a standard curve, a set of samples with a known...

8. Virtual Science Conference Coming Up? Three Addgenies Share Their Experience

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   ...audience engaged. They first sent a pre-workshop questionnaire to the participants to learn about what they...pointed out that networking at virtual conferences requires you to take more initiative. “You have to actively...

9. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

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   ...illuminated with green light. They found that KillerRed required longer exposure times (5 minutes vs 10 seconds...longer exposure times. Additionally, KillerRed requires mitochondrial targeting to increase cell ablation...

10. Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS

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    ...tissue types! Although putting a gene of interest directly downstream of a tissue-specific promoter sounds...34, 1–15 (2002).PubMed PMID: 12324939. 3. S. E. McGuire, G. Roman, R. L. Davis, Gene expression systems...

11. Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

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    ...nuclease. The Streptococcus pyogenes Cas9 (SpCas9) requires an NGG PAM to recognize the target. Osamu Nureki...such as transcription factors tethered to dCas9, require long time scales with constitutively expressed ...

12. Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System

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    ...serotonin. Using a combination of computer modeling, directed evolution, site-saturation mutagenesis, and medium-throughput...anxiety, but these drugs are often ineffective or require several rounds of dose and drug adjustments. Unger...

13. From Lab Bench to Leadership: Transitioning into Management as a PhD

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    ... goals, and fostering team success. This shift requires deliberate preparation and a willingness to embrace... members thrived on independence, while others required more guidance and structure. I quickly learned...

14. Sharing is Caring: But How to Distribute Open Hardware?

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    ...clarified, especially with regard to licensing models. Direct sharing of work from universities and research ...Deepak Krishnamurthy, Ethan Li, Pranav Vyas, Nibha Akireddy, Chew Chai, Manu Prakash, "Squid: Simplifying ...

15. Building and Validating Recombinant Antibodies: A Case Study

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    ...to WGS the lab’s entire hybridoma collection. Instead, the Trimmer Lab moved to direct functional validation...

16. Antibodies 101: Isotypes

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    ...response, the variable region of an antibody is paired with any of the other isotype heavy chain constant...deleted.     Figure 2: Isotype switching requires DNA recombination of the antibody gene locus. ...

17. GPCRs: How Do They Work and How Do We Study Them?

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    ...new ligands for unknown GPCRs. You can find the entire PRESTO-TANGO kit at Addgene! TRUPATH: Bryan Roth...chemogenetics receptors, GPCRs were first mutated via site-directed mutagenesis to bind non natural ligands. These...

18. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

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    ...DNA base changes and consist of a catalytically impaired Cas protein (dCas or Cas nickase) fused to a DNA-modifying...editing target site on the DNA, the Cas domain directs the modifying enzyme to the target site, and the...

19. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

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    ...the research is ongoing, but all registries do require that research plans become public eventually). ...implement authentic scientific research and as the Director of Advising in the Division of Life Sciences at...

20. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

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    ...thus HEPN-independent. These enzymes also do not require a protospacer flanking sequence, so you can target...or processed gRNA array (22 nt spacer with 30 nt direct repeat) mediating potent knockdown. Multiplexing...