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  1. What Good Citizenship Can Do for Reproducibility in Science

    Type
    Blog Post
    ... of being honest and straightforward, it’s an important part of being a “good scientific citizen” and ...Press journals, including Cell Metabolism, Cell Reports and Joule. In September 2017 she moved away from...

  2. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    ...assembly. What makes it so forgiving, is the incorporation of a 3’-5’ exonuclease in the reaction mixture...assembly approaches, and opens the door for new opportunities in the field.  Gibson Assembly® is a registered...

  4. Components of CRISPR/Cas9

    Type
    Blog Post
    ... combined with the tracrRNA forms the scaffold portion of a gRNA and the “spacer region” forms the target...

  5. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    ...the right candidate for the job. How can you incorporate the skills developed during graduate school into...whether to stay in academia or not, it is even more important to start early. Think about your plan for the ...

  6. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    ...binding. Suzuki et al. also envision eNLs as good reporter genes for endogenous protein fusions created using... substrate is required for imaging, and it is important to verify that this substrate does not alter cellular...

  8. Screening for Successful Genome Editing with Digital PCR

    Type
    Blog Post
    ...detection limits of about 5% mutant targets have been reported for mismatch nuclease assays (2, 6, 7). Ability...nuclease assays are completely “blind” to this very important difference (Figure 3). If you are interested...

  9. Early Career Researcher Toolbox: Social Media for Scientists

    Type
    Blog Post
    ...NPR, articles in magazines like Fast Company, opportunities to speak at conferences, and offers for postdoc...can fit into a researcher’s work day. It’s also important to remember that it’s not the amount of time you...

  10. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    ...from the mol bio side you’re sure to have many opportunities to learn about and develop technologies that...FRET-based biosensing technique can be applied to an important neuronal signalling pathway - if you’ve got a ...

  11. Hot Plasmids - February 2022

    Type
    Blog Post
    ... provides a useful yet low-pressure teaching opportunity for lab members or students new to protein expression...induce high levels of gene activation for both reporter and endogenous genes. It is highly specific in...

  12. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ...the target cell or transiently expressed. It’s important to note that for all the applications we discuss... plasmids can induce gene-specific silencing temporarily, long-term, and even inducibly depending on the...

  13. Clear and Accessible Writing

    Type
    Blog Post
    ...reader would glean from the image itself.  As we incorporated alt text into our work, we began to realize ..., and we’d love to hear yours as well.  More importantly, you can use these four steps for any type of...

  14. How Addgene Creates Content

    Type
    Blog Post
    ...conversations, conferences, and questions our scientific support staff receives – in fact, the blog originally started... post (email [email protected].) We are here to support and advance the scientific community, and we can...

  15. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    ...aging. For both a screen and a selection, it is important to clearly define what type of factor you are ...whole genome), they knock down gene expression temporarily. RNAi libraries are especially useful if you ...

  16. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    ... cell membrane. Heterotrimeric G proteins are important molecular switches in signal transduction pathways...which can then activate expression of a luciferase reporter in the nucleus. The Roth lab showed that this ...

  17. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    ...sequence flexibility indicates that gRNAs could be incorporated into these constructs without compromising cleavage...slightly higher mutation rates than they’d previously reported in protoplasts (15% and 9% of target sites.) How...

  18. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    ...process involve repairing chromosomal DSBs. It is important to know that cells possess two major repair pathways...chance of causing a frameshift mutation. Of some importance, the deletion can be less heterogeneous when ...

  19. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    ...): These are considered low copy vectors and incorporate part of an ARS along with part of a centromere...nutrient, the host cells will die unless they have incorporated the plasmid carrying the required gene.  The...

  20. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    ...plasmids have been introduced by transfection or electroporation. All you do to create a protein that binds ...where particular variants or mutations have been reported in people, we can use this kit to knock in those...
Showing: 621 - 640 of 916 results