Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 922 results for: tro

Showing: 621 - 640 of 922 results

  1. Cultivating Community Science at BosLab

    Type
    Blog Post
    ... the lab, so in December 2015 we held our first intro to bacterial transformation course (read our blog...bacterial transformation guru and instructor for our intro to biohacking courses. Our members pursue a diverse...

  2. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    ...Nsp5. Our set also includes a GFP construct as a control. For easier portability, all viral genes are flanked...clone selection. Expression is driven by a fairly strong EF1alpha promoter. We have verified expression ...

  3. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    ... one figure. If you aren’t there yet, write introductions to potential publications or prepare a review...isn’t for a while. Getting started early on introductions and literature reviews could make the months...

  4. Networking Follow-up: I Met a Person. Now What?

    Type
    Blog Post
    ...person or virtually) will make the relationship stronger.  If appropriate, invite them to speak for your...chocolate-covered strawberries for helping with an introduction that helped someone get a job interview. Even...

  5. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    ...necessarily have to be. Regions of homology can be introduced to fragments via PCR using primers that contain...patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc. Many thanks to ...

  6. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    ...you did. Emphasize the skills. If you studied astronomy or bioinformatics data, then the chance is that...Communications Coordinator for the Department of Physics & Astronomy, University of British Columbia. Originally on...

  8. Advice for Scientists Starting a Lab

    Type
    Blog Post
    ...equipment always helps. But how does one build a strong collaborative research team motivated by similar...selectively picking the first couple of hires to build a strong, collaborative team. And take advantage of the ...

  10. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    ...Please keep in mind that this is just a brief introduction to the wide, wild, and fascinating field of ...Antibodies 101: Polyclonal Antibodies Antibodies 101: Introduction to Antibodies Resources on Addgene.org ...

  11. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

    Type
    Blog Post
    ...base pairing between these ~4 base overhangs isn’t strong enough to hold a plasmid together through transformation... reading Harnessing homologous recombination in vitro to generate recombinant DNA via SLIC. Li MZ, Elledge...

  12. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    ...already grown out of our workshops. If you're having trouble finding science communication opportunities in ...of its Leadership Team.  Nathan did his PhD in astronomy and works as a data scientist in the entertainment...

  14. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type
    Blog Post
    ..., you’ll want the proper positive and negative controls for your PCR.  Find the details and learn more... this blog post for tips for analyzing and troubleshooting Sanger sequencing results. At Addgene, we now...

  15. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    ... Image from Wen et al., 2017. Once you’ve introduced the reporter into the cells, you’re ready to ...sequence is also found in the reporter gene). A control RFP expression plasmid.  With this set up, all...

  17. A Deep Dive into BioBuilder

    Type
    Blog Post
    ...extracts to perform transcription and translation in vitro, and the new BioBuilder module allows students to...Assembly Standards Find Plasmids for Creating and Controlling Gene Networks Browse All SynBio Plasmids ...

  19. Screening for Successful Genome Editing with Digital PCR

    Type
    Blog Post
    ...minimally informative. In this blog post, we’ll introduce digital PCR as an emerging validation technology...assays for mutation screening Now that we have introduced how digital PCR works, let’s explain what kind...

  20. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    ...team develops methods, performs extensive quality control, generates  educational materials, and provides...details about virus production protocols and quality control can be found on Addgene’s Viral Production webpage...
Showing: 621 - 640 of 922 results