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We narrowed to 1,010 results for: RING;

Showing: 321 - 330 of 1010 results

  1. Sensing Neuronal Dopamine

    Type
    Blog Post
    ...Methods for measuring Dopamine In vivo microdialysis The gold standard method of measuring DA and other...someone is awake, agitated, or asleep. Though DA firing patterns (or the frequency of its release) are ...brain slices Visualization of DA release dynamics during locomotion in freely moving mice Record DA dynamics... in live mice Report modulation of DA signaling during learning and reward consumption in live mice Find...of our viral vectors blog posts Find tips for preparing AAVs Download our viral vectors 101 eBook Resources...

  2. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

    Type
    Blog Post
    ...fluorescent proteins were best imaged after tissue clearing with SeeDB2 (Ke et al., 2016, 2018).   How to...amount of tTA to achieve highest expression levels. Clearing large tissues - Fluorescent proteins are very ...with ScaleCUBIC1 (Susaki et al., 2014), before clearing with SeeDB2. For more information, visit our ...action, watch the video below depicting dendrite wiring of mitral cells.   Many thanks to our guest... of neuronal circuitry with an index-optimized clearing agent." Cell reports14.11 (2016): 2718-2732. PubMed...26972009. Ke, Meng-Tsen, and Takeshi Imai. "Optical clearing and index matching of tissue samples for High-...

  3. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

    Type
    Blog Post
    ...vector into the culture medium during adeno-associated virus manufacturing." Human gene therapy21.10 (2010...Enhancement in vector stability." Biotechnology and bioengineering 94.2 (2006): 322-329. PubMed PMID: 16528756...pressure and sodium butyrate." Biotechnology and bioengineering 42.5 (1993): 601-610. PubMed PMID: 18613082...and antibody production." Biotechnology and bioengineering 37.10 (1991): 989-993. PubMed PMID: 18597326... Kamen. "Hyperosmotic pressure on HEK 293 cells during the growth phase, but not the production phase,...

  4. Addgene Begins Distribution of Recombinant Antibodies

    Type
    Blog Post
    ...from Addgene. How Addgene is Reimagining Antibody Sharing Addgene is building our antibody resource, which...community-building. The transparency begins with sharing sequence information for the antibodies in our ...accompany our antibody collection.  Addgene is sharing our protocols for recombinant antibody production... antibodies for your research. Please consider sharing your experiences with antibodies from Addgene via...other. Together, we can transform the antibody sharing landscape as well.     References Andrews NP, ...a nonprofit that has been supporting hybridoma sharing within the community for decades. Educational Resources...

  5. Celebrate Open Data Day with Addgene’s AAV Data Hub!

    Type
    Blog Post
    ...We’re big fans of all forms of research sharing and with Open Data Day 2020 coming up on March 7th, what... what better way to celebrate open data sharing than with Addgene’s AAV Data Hub! We launched this pilot...ranks of our growing group of Addgene AAV data sharing champions? If you’ve recently used Addgene AAV ...simple steps you can take to input your data for sharing on the site. The entry form has 5 general question...questions and so that we can give you recognition for sharing your data. Materials: What virus and serotype did... have any feedback at all, we’d appreciate you sharing it through this form. In honor of Open Data Day...

  6. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    ...reagent-grade endangered Martian chicory root, but considering this is a needle in a haystack type of objective...In retrospect I used a way too high Cas9 MOI considering that A549 cells are relatively easy to transduce...could avoid potential off target artifacts by comparing the phenotypes of multiple gRNAs. Targeting different...lanes 0.3 % 3.0, Figure 5, lane 2) is faint, so comparing it to the undigested control (Figure 4, lane 0...straightforward, and is a testament to how far genome engineering has come. For comparison, in 2007 it took about...for Designing Primers UCSC Genome Browser: For eploring genomic DNA ImageJ: Free Software for Analyzing... genome engineering...

  7. Finding Your Science Policy Path

    Type
    Blog Post
    ...ranges from astronomy to bioethics to chemical engineering and everything in between. Plus, there are many...pursue it. It can manifest via research and data gathering, regulatory efforts, policy implementation, program...societies host “Hill Days” where they train and bring researchers to Congress to advocate for increased...normal!  Action Items:  Start a career journal during your current job and note what makes your heart...writing and presenting for non-scientist audiences during your time in graduate school through outreach organizations...working in student government, and teaching and mentoring others (see more in Figure 2). Starting early ...programs can connect you with alumni before and during your application process so you can learn more!...

  8. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    ...translation is key to cellular function, especially during development or stress. With ribosome profiling,...to track translation in two different ways: by monitoring the first round of translation or by tracking...NLS-MS2-RFP) the reporter mRNA, which contains the tethering sites for the fluorescent proteins and is driven...double labeled, indicating translational repression. During later stages, single RFP-labeled osk and Oskar ...with the red reporter mRNA. Yan et al. used harringtonine, a translation inhibitor that stalls ribosomes... Mary Gearing...

  9. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    ...how it works. I used to stay up past my bedtime, poring over my grandparents’ medical textbooks by torchlight... I asked participants to read short abstracts comparing the mortality rates between two controversial ...and biased. As a result, the control abstract comparing two fictional medical issues – chronic and acute... Kahan et al., 2012). Scicomm in the community During my PhD, I’ve also found opportunities for more ...community. I brought along printed copies of studies comparing the filtration efficacy of different materials... in technical jargon that even scientists in neighbouring disciplines might struggle to understand. Why...

  10. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    ...introduced by lambda red recombineering and Multiplex Automated Genome Engineering (MAGE) will always be ...methods for bacterial genome engineering   Learn more about lambda red recombineering of bacteria Use site directed...within a defined region of a genome. Require stringent bacterial growth conditions. Expression of an ...only increasing the standard mutation rate seen during DNA replication by 120-fold over background. Due...
Showing: 321 - 330 of 1010 results