...regeneration studies, and cancer biology. I was first introduced to bioelectricity when I attended the Developmental...living cells. Since bioelectrical signals are controlled by the opening and closing of ion channel proteins...genetically encoded, light-gated ion translocators to control tumorigenesis." Oncotarget 7.15 (2016): 19575. ...signaling networks: exploiting voltage gradients for control of growth and form." Annual review of biomedical... neural and gap junction protein-mediated cues control polarity during planarian regeneration." Developmental...Vaibhav P., et al. "Transmembrane voltage potential controls embryonic eye patterning in Xenopus laevis." Development...

...research that was done and what it means. The introduction should give you just enough information to allow... addressed. In primary research papers, the introductions should be fairly focused. Consult a review article... etc)? Did they perform all of the appropriate controls? Refer back to the methods section for finer points...automate the process as well. Sign up for eTOC (electronic table of contents) alerts from journals you read...useful papers. For many, this collection is purely electronic. Gone are the days of file cabinets filled to...She is a fan of photosynthesis and writes about autotrophs and biochemistry on her blog. Follow her on Twitter...

...approach of CeNGEN, check out their publication.   3’UTRome The 3’UTRs of mRNA are important for post-transcriptional...expression in C.elegans. In this system a promoter controls the expression of a GAL-4 driver that can bind...also find additional SapTrap plasmids used for introducing point mutations that was published in a microPublication...Central PMCID: PMC3531189. Mangone, Marco, et al. "UTRome. org: a platform for 3′ UTR biology in C. elegans... L., & Jorgensen, E. M. "SapTrap vectors for introducing point mutations with unc- 119 + selection." MicroPublication...split intein for refined spatiotemporal transgene control in Caenorhabditis elegans." Proceedings of the ...

...activity in HEK293 by reducing editing from ~30% in control samples to 0-10% in Acr samples. Acr proteins can...When added to human cells six hours after the introduction of Cas9 RNPs, AcrIIA4 reduced off-target editing...specific site. 2. Temporal, spatial, or conditional control of CRISPR activity Regulating Acr proteins by inducible...molecules could achieve rapid, dynamic, and spatial control of CRISPR-Cas activity. For example, fusing AcrA4II...LOV2 domain allows anti-CRISPR activity to be controlled by blue light (Bubeck et al., 2018). 3. Reduce...Your Next Experiment Read more about Optogenetic Control of CRISPR   Resources on Addgene.org Find CRISPR...

...response (e.g., graft vs. host) as well as the introduction of the cancer-fighting component, CAR. Clinical... edit with the goal of ‘fixing’ mutations or introducing a desired edit, but this particular class of ...of treatments has quite the opposite aim: to destroy. One treatment currently in clinical trials includes...but are not limited to, ones targeting muscular dystrophy, cardiovascular disease, inflammatory diseases...by Kendall Morgan and updated in 2023 by Susanna Stroik.  References and Resources References Yuxuan Wu...blog CRISPR Antimicrobials Treating Muscular Dystrophy with CRISPR CRISPR 101: Drug Discovery and CRISPR...

... calcium binding proteins (e.g., calmodulin or troponin C). Their fluorescent readouts are used to monitor... is a FRET-based calcium reporter derived from troponin C. It is engineered with a reduced number of calcium...fluorescent state only by simultaneous (1) user-controlled illumination with violet light and (2) exposure...different versions optimized for neuron-specific, astrocyte-specific (GCaMP6 only) or mammalian cell expression...101: Viral Applications Viral Vectors 101: An Introduction to AAV Find AAV Calcium Biosensor Vectors at... Susanna Stroik...

...Tips for Plasmid Quality Control Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene Bioinformatics... has many points at which small errors can be introduced. Eventually, these errors could cause the plasmid...Figure 1. Potential points where DNA errors can be introduced, indicated by exclamation marks. Error points...Addgene, we put a lot of effort into our quality control (QC) processes to screen for and reduce these errors...non-negotiable. By maintaining a high standard of quality control, we have built a trusted plasmid catalog containing...

...are monoclonal antibodies that are generated in vitro using synthetic genes that are typically expressed... a patient’s blood), scientists can use mass spectrometry to determine the amino acid sequences of the...process of antibody selection and maturation in vitro by selecting for antigen binding from libraries ...or mammalian display (Tsuruta et al., 2017). In vitro selection allows scientists to create antibodies...into an expression plasmid, the plasmid can be introduced into host cells, such as bacterial, yeast, or...on the Addgene blog Read our Antibodies 101: Introduction to Antibodies blog post Learn more about hybridomas...

...101 eBook! Researchers use various techniques to control protein expression for experimental, biotechnological...successful. Some mammalian systems also allow for control over when a protein is expressed through the use...Protein expression from each of these vectors is controlled by a different promoter resulting in different...to E. coli. Of all the vectors, pET, under the control of the T7 lac promoter and induced by lactose, ...researchers often place recombinant proteins under the control of the galactose inducible promoter (GAL). Other...cell-free expression systems, proteins are assembled in vitro using purified components of the transcription and...

...placing FP timer DsRed-E5 under the control of Neurog3 (a gene controlling pancreatic differentiation) in ... (500 to 580 nm) over the course of 18 hours in vitro (see figure 1B in Terskikh et al.) and even displays...showed that their versatile timer could be used in vitro, in mammalian cells, in C. elegans, and in Xenopus...synthesized or long-lived transcription factors control gross changes in gene expression as new body sections...Pratik, et al. "A programmable synthetic lineage-control network that differentiates human IPSCs into glucose-sensitive...