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We narrowed to 968 results for: LAT;

Showing: 641 - 660 of 968 results

  1. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    ...measurement. Like a p24 ELISA, this method is relatively quick, providing results in less than a day, ...amplification of the original plasmid which can over-inflate results. A packing vector deficient in reverse ... serial dilutions of a viral prep, genomic DNA isolated, and primers targetting viral components or the...

  2. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    ...protecting intellectual property, it worked away in isolation. It published a paper a year (or less) and limited...popped into our heads. Anything was fair game for speculation - in fact, our color changing flower project ... accomplish the color change until a few months later. RevBio accelerates with help from SynBio Axlr8r...

  3. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    ...managing a team. You’ve just been promoted, congratulations! You’re now a manager with your own team! But... other student stayed until he graduated. Years later I attended a formal training for new managers and...you motivated and help you grow in your role. The latter will make you feel discouraged and not valued which...

  4. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    ... other Cas effectors can’t, while maintaining relatively high efficiency. These alterations are achieved...Cutting both strands ensures that there isn’t a template strand left behind that the cell can use to repair...combination of chewing up one stand and cutting the template strand causes large deletions of up to hundreds...

  5. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

    Type
    Blog Post
    ...system. The paper also gives quantitative data for regulatory elements, which has not been available for plants...Nicotiana benthamiana. N. benthamiana is a tobacco relative and a good model for plant disease because it ...Patron: Remember plants. Every time you look at your plate, think about how much that’s on it are plants, and...

  6. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    ...particular scientific subfields.  Plasmid requests correlate with paper publication The dataset includes 11,426...and uncertainty in estimating parameters from relatively sparse data from China. An important additional...therapeutics. Prime editing, base editing, PACE, and DNA-templated synthesis are four examples of technologies pioneered...

  7. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

    Type
    Blog Post
    ...Thanks to the Beer–Lambert law, the instrument can calculate a sample’s nucleic acid concentration based on... reading, distorting the final concentration calculation and potentially impacting downstream experiments...systems to better identify contaminants and to calculate corrected concentrations. However, such systems...

  8. Getting to Know Expansion Microscopy

    Type
    Blog Post
    ...they want to understand the ultrastructural relationships that influence everything from cell division...physically separates molecules while maintaining their relative positions, allowing you to resolve individual ...accessible imaging equipment, ExM protocols utilize relatively inexpensive reagents and are readily adoptable...

  9. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...mammalian cells can perform human-like post-translational modifications that bacterial and most yeast ...also need to know the extinction coefficient to calculate the concentration. At 280 nm, the extinction coefficient...protein standard and the concentration may be calculated using imaging software. Any bands on the gel ...

  10. Negotiating Work and Life: How to Find the Joy

    Type
    Blog Post
    ... If you are miserable at home or work, your relationships will suffer in both the short run and the long... learn to work within these bounds You can work later (in the evening, or in a few years) when life responsibilities...agree on before you get enmeshed in a serious relationship. #5 Thou shalt not sweat the small stuff The...

  11. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

    Type
    Blog Post
    ...several genes previously not associated with ricin regulation, including almost all genes involved in the production... screen did fail to identify some known ricin-regulators, but most of these genes are also essential for...increased and decreased p-values of known ricin regulation genes compared to results generated using non-targeting...

  13. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

    Type
    Blog Post
    ...singletons). I felt like anyone at a meeting who felt isolated at a conference for any reason was a Singleton...phenomenon in bacteria that occurs when bacterial cells regulate gene expression in response to changes in cell...structure with regard to the Mentor-Singleton relationship. Some of the variability was organic, as some...

  14. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    ...modifiers available. This system utilizes an RNA template containing the desired edit adjoined to the sgRNA...affect gene expression? dCas9 can be used as a platform to deliver various cargo to a specific DNA locus...single transcriptional activation domain - VP64.  Later generations of activators include the SunTag system...

  15. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    ...different bacterial hosts (Doran et al. 1998). Regulatory elements within the ori also control copy number...which is the most commonly used bacteria in lab manipulations. Table 1 has a detailed list of the bacterial...leguminosarum, R. meliloti, Rhodobacter sphaeroides, R. capsulatus, Rhodopseudomonas spheroides, Salmonella spp....

  16. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ...cells with VSV-G pseudotyped virus requires the upregulation of LDLR expression using other methods.  In ...Gutierrez-Guerrero, et al, 2020). Because retrovirus is closely related to lentivirus, many retroviral envelope proteins...spumaviruses to human serum by galactosyl(alpha1-3)galactosylation. Journal of virology 71:6174–6178 . https:/...

  17. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    ...targeting the genomic site of interest. The donor template is supplied separately. To use this system, you... 37 °C.     The new pEcCas/pEcgRNA system We speculated that the gRNA on pCas that is specific to the... in the lack of recovered transformants. Our speculation motivated us to update the pCas/pTargetF system...

  18. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    ...ummmm, what is it? Reddit is a volunteer-organized platform where users can participate in forum discussions...to see the questions first makes Reddit a great platform for those who aren’t so great at coming up with...whole point is to meet actual people to build relationships. I also like Twitter because I can get a little...

  19. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    ...path, you’ve probably got a gene you’d like to manipulate in mind. Now it’s time to consider where you ...mice once they have been generated (more on that later). Find Validated gRNAs to Target Mouse Genes To ...biology and genetics. She loves to communicate the latest and greatest in scientific content.      References...

  20. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ...enough to promote cell growth. Overgrown cultures stimulate quiescence, the resting phase of the cell cycle...that the results obtained in these lines can be translated to other lines. Differences in the physiology...therefore titers obtained in one cell type may not translate well to other cell types. To overcome this issue...
Showing: 641 - 660 of 968 results