...surprised at how many applicants ignore this in their cover letters. It is also important to express interest... Many job postings will result in hundreds of applicants the day they are posted.  If your resume is not...wish list and the hiring manager knows that no applicant is likely to meet all the criteria. Note the criteria... to highlight different skills and to get the important information in the right place on the page. 5)...

...protocols, including vector-insert joining for recombinant plasmid construction (restriction cloning), adaptor...Numerous applications have been developed to take advantage of the high fidelity of Taq and other thermostable...nucleotide sequences with high specificity and quantitative accuracy and even include profiling single nucleotide...However, some ligases can ligate mismatches to a significant degree, and very active ligases, such as T4 DNA...different length, with products identifiable and quantifiable by capillary electrophoresis (CE) (see below...commercially available (see below). Conclusion It is important to note that thermostable, high fidelity, nick-selective...

...with the relevant metadata and the screen results. Screenshots updated 2024. Importantly, metadata...treatment can browse through screens performed in relevant cell lines or conditions. This increased ease ...enzyme system, sgRNA library and other details relevant to screen result interpretation. The analytical...   Screen results are reported as the original quantitative gene-level scores provided by authors rather...

...features you may want to consider as you make your choice. The first and most important thing you need ... achieved with the proper selection marker or antibiotic resistance. Next, consider the expression level... or purify it – and you do not have a specific antibody against the protein available – you can always...be a more robust expression system and has its advantages; however, remember that you will need accessory...

... you may want to work on in your future career, so the research topic will be more important. In addition...selecting a lab with a PI who is a good mentor is important in both cases. As a PhD student you need to learn...scientists. The specific research area is not as important as choosing a supportive, positive role model.... opportunities to learn and practice successful grant writing, lab budgeting, equipment acquisition, public...

...your formal scientific communications, they are fantastic fodder for science communication. The trick is...something that sparks their interest and makes them want to know more. This could be by outright stating ...story.  At this point you may be thinking  “I don’t want to bury my results all the way at the bottom of ...other affected people, man/animal vs nature, anthropomorphized animals, or even seemingly unrelated stories...storytelling devices, we sometimes forget the most important thing about science: It’s cool. Like bowties! ...

... bacterial culture, only the plasmid with the antibiotic resistance gene and origin will replicate, and....7 kb recombined vector backbone contains the antibiotic resistance gene (AmpR) and origins of replication...Growth Conditions To reduce recombination, it is important to not overgrow the plasmid at 37°C. Some researchers...(up to 24 hr), reducing the concentration of antibiotics (ie. 50 μg/mL Amp instead of 100 μg/mL Amp), ...MK, Rudolph C (2019) Segmented poly(A) tails significantly reduce recombination of plasmid DNA without ...

...share your AAV data Do you use Addgene AAV tools and want to join the ranks of our growing group of Addgene...Fluorescence? Immunohistochemistry? Did you use any relevant animal treatment conditions like drugs (Chemogenetics...designing your experiment, read our blog post on important considerations when using AAVs. Experimental results... we also take negative data, which is equally important to report. We recently published a paper in Frontiers... hub! Related resources on the Addgene blog Important Considerations When Using AAVs Three Key Considerations...

...monomeric variants of Clavularia cyan fluorescent protein: identification of the molecular determinants of color... coli with a gene library of approximately 484 variants, including some potential tetramer-breaking mutations.... mTFP1 is also insensitive to physiologically relevant changes in pH. Find mTFP1 plasmids here! mWasabi...excitation maximum 399 nm) because it does not significantly excite at 400 nm while EGFP does. Find mWasabi...

...pH-insensitive RFP variant, DsRed.T3, that’s connected to a pH-sensitive GFP variant, super ecliptic pHluorin...makes Keima useful for monitoring mitophagy. It’s important to remember that because Keima’s fluorescent relies...fixed cells. Find the mitochondria-targeted Keima variant at Addgene! mRFP-GFP-LC3 tandem fluorescent protein... Noda, Takeshi, and Daniel J. Klionsky. "The quantitative Pho8Δ60 assay of nonspecific autophagy." Methods...

...NgAgo In eukaryotes, Argonaute proteins play an important role in RNA interference, binding RNA guides to...replicated in larger studies, it will represent a key advantage over Cas9, for which gRNA sequence can greatly...aa depending on the species). This feature is advantageous if you’re working with small capacity viral ...via indel formation, this property could be an advantage. However, repair after base excision would alter... Cas9 Homolog Cpf1 Check Out High Fidelty Cas9 Mutants Browse All Genome Engineering Posts Additional...

... laptops and some server space. But what if you want to be an entrepreneur in the health sciences?  Is...The composition of the management team is more important than the product itself -- investors would rather...majority of these meetings acted as a platform for significant company advancement. Be relentless The world ...against you.  As I mentioned, you start at a disadvantage in the health science space if you don’t have... to it. First impressions are everything and you want to leave people remembering your potential as an...

...used to negotiate individual MTAs every time they wanted to share materials, and the process was time-consuming...facilitate a fast and agile MTA process. This is important to keep up with the number of requests and deposits...sharing process smoother, more consistent, and more pleasant for everyone, which in turn encourages people ...process.  And you do have options for the terms you want to share your biological materials under! While ...for both research and commercial purposes. It’s important to note that it’s the depositing institute that...

...connections Here are a few things you probably don’t want to do: Don’t connect via LinkedIn with strangers... for favors.  Don’t focus on networking with “important” or senior people. Focus on building relationships...times if you don’t get a response. It breaks an important law of networking--don’t be creepy. There is a...4,700 LinkedIn connections. I mention this because I want it to be clear that it is completely impossible ...interest in you. Network with the people that you want to be with. Then, the following up will be easy....

... of being honest and straightforward, it’s an important part of being a “good scientific citizen” and ...hurdle in front of publication when all they really want to do is get the paper off their desk and move on...only a partial solution.  How do we get where we want to be? The part that is still missing is a clear...management plans and for evidence of sharing for grant renewal—but there is clearly room to do more. If.... Sounds like a win for everyone. If we agree we want to get there, that’s half the battle. Many thanks...

...plexWell™’s sequential tagging procedure, the quantities of library molecules for each sample remain highly...plate) will typically have a wide range of input quantities. This variation is problematic for conventional... individual libraries can be influenced by the quantity or quality of input samples. To minimize these...from multiple samples will then also need to be quantified and re-normalized in order to be combined with...proprietary chemistry). This turns out to be critically important for getting adequate sequencing depth and coverage...

...great for lab life. These apps prevent you from constantly searching the interwebs for lab resources, and... Lab Archives by LabArchives The single, most important part of lab life is the lab notebook. Lab Archives...always comes first with research! It is always important to know what kind of reagents you work with in...MSDS for a lab chemical of choice. Now, I can’t guarantee every reagent in your chemical closet is in this...iGreenEarth AMINO is a very simple app that packs important biological information, amino acid abbreviations...

...community, so it is extremely important for us as a company to constantly update our processes and get ...for Fragmentation System and this is the most important part of this newly released prep kit. In their...itself. This was great because it forced workshop participants from different biotech companies to interact...Therapeutics, a biotechnology company that uses neoantigen biology to treat cancer. My partner for the day...

...growing the cells on solid plates, where each transformant forms its own colony, uninfluenced by the growth... from both. These products are then purified, quantified, and sent for sequencing. Analyzing your sequencing...corresponding sequencing quality. Generally, you want to have 100x-1000x coverage of your library: in ...very similar to each other - that’s exactly what we want! At the end of the day, it’s up to you to decide...hope this post has helped clarify some of the important considerations when using, amplifying, and verifying...

...avenues previously unavailable to them. With these fantastic tools it’s now easy to monitor protein expression...Zhou and Fatima Enam. There were many excellent applicants but Xin and Fatima stood out for their creativity...precisely spatio-temporally controlled as a key advantage over other inducible enzyme technologies. She ...Oligosaccharides (HMOs). HMOs are thought to play an important role in nuturing the development of beneficial...communication skills throughout her training and wants to make her work understandable to people both inside...