We narrowed to 964 results for: APP
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TypeBlog Post...kinetics) that makes them suitable for different applications. We recently released a new variant of jGCaMP7... been developed and are suited for different applications. The iGABASnFR GABA sensor (pAAV.hSyn-FLEX.iGABASnFR...
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Arabidopsis in Education: How the Arabidopsis Biological Resource Center Brings Plant Science to Life
TypeBlog Post... Biological Resource Center and the Center for Applied Plant Sciences at The Ohio State University. Established... Biological Resource Center and the Center for Applied Plant Sciences at The Ohio State University. In... -
CRISPR Challenges: Standardization and Homology Directed Repair
TypeBlog Post...the gRNA design process and rules for various applications is necessary going forward. The standardization...Alt-NHEJ), has been harnessed for CRISPR knock-in applications. CRISPR 101: Targeting RNA with Cas13a (C2c2... -
Viral Vectors 101: What is a Virus (and a Viral Vector)?
TypeBlog Post...into two cells. Viral vectors Viruses are quite appealing as molecular biology tools… if one isn’t too bothered...on the Addgene blog Viral Vectors 101: Viral Applications Viral Vectors 101: Viruses as Biological Tools... -
What Do I Do Now? Academic v. Non-Academic Career Decisions
TypeBlog Post...can do better, no grant writing Job changes can happen regularly (every 4-6 years is common), but this...issues can create interpersonal issues (one bad apple can change entire culture) Specific requirements... -
Plasmids 101: TOPO Cloning
TypeBlog Post...“A” overhang insert, the magic of TOPO cloning happens. Basic procedure Let’s break down the steps needed...1658796. PubMed Central PMCID: PMC52876. 2. Novel approach to molecular cloning and polynucleotide synthesis... -
Identifying Sequence Elements with SnapGene's Feature Database
TypeBlog Post...response to customer feedback. An example of this approach is SnapGene’s algorithm for detecting common features.... We are working on a way for SnapGene users to apply features from a reference strain to newly sequenced... -
Donations from Addgene to Yield Answers for Rare Disease Researchers
TypeBlog Post...on OCRL1 already underway in his lab. The gene appears to play a role in many cell processes involving...In Thailand, our annual operating budget is approximately 20,000 USD and we are working on several genetic... -
Don’t Wait To Launch Your Health Science Startup
TypeBlog Post...would apply a percentage of returns to support student projects, but it quickly became apparent that raising... -
Another Pathway into Cells: iTOP
TypeBlog Post...protein-only transduction method and its potential applications to CRISPR/Cas9 genome editing. Protein-only...Diego D'Astolfo. CRISPR/Cas9: a potential application of iTOP Having tested iTOP using Cre-lox recombination... -
Extracurricular Activities for a Strong Science Career Path
TypeBlog Post...multidisciplinary, collaborative, and translational approaches for scientific tool and technique development...leadership, management, and communication. A great approach to fully understand and acquire competitive skill... -
Celebrate National Book Month with Addgene
TypeBlog Post...science. It's a series of case studies in what happens when scientists bring biases to research and don't...English, I had lapsed. Baker’s novel reignited my appreciation of poetry, and I’ve been reading a poem a day... -
Improving Transduction Efficiency with Exosome AAVs
TypeBlog Post...exosomes, small membrane vesicles or microvesicles (approx. 40 - 150 nm diameter) that are secreted from the... This is particularly useful for therapeutic applications in vivo where endogenous anti-AAV antibodies... -
Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids
TypeBlog Post...referred to objects that exist online, but this approach has been adapted for the identification of physical... Addgene plasmid URLs act as unique PIDs, we’re happy to support the RRID and compact identifier formats... -
Reagent Repositories Are Speeding up Science During the Pandemic
TypeBlog Post... Another example of reagents that have broad applications but have proven useful to the study of SARS-...et al., 2006). Now that a similar phenomena is appearing with regards to SARS-CoV-2, this mouse strain ... -
The Importance of High Titer for AAV Transductions
TypeBlog Post...for additional best practices. Keep your cells happy - Make sure your cells are as healthy as can be ...AAV-mediated anterograde transsynaptic tagging: mapping corticocollicular input-defined neural pathways... -
The MTA at Addgene
TypeBlog Post...years! This has helped us develop a fast, agile approach, which we’ve updated and refined as new technology...and our MTA YouTube video for more information. Happy transferring! Many thanks to our Tech Transfer... -
How to Lead a Great Meeting
TypeBlog Post...assign each one using group members’ initials. If appropriate, email this around after the meeting. Accountability...Remember, many of the more in-depth discussions can happen “off-line” with just one or two relevant people... -
Components of CRISPR/Cas9
TypeBlog Post...gRNA) and their prefered Cas9 variant (with the appropriate PAM sequence) to modify the desired genomic locus... more to be found in the full CRISPR 101 eBook. Happy Reading! ... -
Synthetic Photobiology: Optogenetics for E. coli
TypeBlog Post...; only two plasmids are required per system. Applications of these systems The large dynamic range of ...suitable for various scientific or engineering applications. References Refactoring and optimization ...