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Showing: 641 - 660 of 964 results
  1. New and Upcoming Viral Vectors - Spring 2019

    Type
    Blog Post
    ...kinetics) that makes them suitable for different applications. We recently released a new variant of jGCaMP7... been developed and are suited for different applications. The iGABASnFR GABA sensor (pAAV.hSyn-FLEX.iGABASnFR...
  2. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    ...into two cells. Viral vectors Viruses are quite appealing as molecular biology tools… if one isn’t too bothered...on the Addgene blog Viral Vectors 101: Viral Applications Viral Vectors 101: Viruses as Biological Tools...
  3. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    ...“A” overhang insert, the magic of TOPO cloning happens. Basic procedure Let’s break down the steps needed...1658796. PubMed Central PMCID: PMC52876. 2. Novel approach to molecular cloning and polynucleotide synthesis...
  4. Another Pathway into Cells: iTOP

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    Blog Post
    ...protein-only transduction method and its potential applications to CRISPR/Cas9 genome editing.   Protein-only...Diego D'Astolfo. CRISPR/Cas9: a potential application of iTOP Having tested iTOP using Cre-lox recombination...
  5. Extracurricular Activities for a Strong Science Career Path

    Type
    Blog Post
    ...multidisciplinary, collaborative, and translational approaches for scientific tool and technique development...leadership, management, and communication. A great approach to fully understand and acquire competitive skill...
  6. Celebrate National Book Month with Addgene

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    Blog Post
    ...science. It's a series of case studies in what happens when scientists bring biases to research and don't...English, I had lapsed. Baker’s novel reignited my appreciation of poetry, and I’ve been reading a poem a day...
  7. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    ...exosomes, small membrane vesicles or microvesicles (approx. 40 - 150 nm diameter) that are secreted from the... This is particularly useful for therapeutic applications in vivo where endogenous anti-AAV antibodies...
  8. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    ...for additional best practices. Keep your cells happy - Make sure your cells are as healthy as can be ...AAV-mediated anterograde transsynaptic tagging: mapping corticocollicular input-defined neural pathways...
  9. The MTA at Addgene

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    Blog Post
    ...years! This has helped us develop a fast, agile approach, which we’ve updated and refined as new technology...and our MTA YouTube video for more information. Happy transferring!    Many thanks to our Tech Transfer...
  10. How to Lead a Great Meeting

    Type
    Blog Post
    ...assign each one using group members’ initials. If appropriate, email this around after the meeting. Accountability...Remember, many of the more in-depth discussions can happen “off-line” with just one or two relevant people...
  11. Components of CRISPR/Cas9

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    Blog Post
    ...gRNA) and their prefered Cas9 variant (with the appropriate PAM sequence) to modify the desired genomic locus... more to be found in the full CRISPR 101 eBook. Happy Reading! ...
  12. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    ...; only two plasmids are required per system. Applications of these systems The large dynamic range of ...suitable for various scientific or engineering applications.   References Refactoring and optimization ...
Showing: 641 - 660 of 964 results