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TypeBlog Post... the future, this stitching technique could be applied to other epitopes and scaffolds to build a more...antibodies for extracellular and intracellular applications: CDR grafting to stable frameworks and structure-based...
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Summer SciComm: Science Storytelling
TypeBlog Post... work. And while these pieces aren't always appropriate for your formal scientific communications, they...still get invested in the journey? The same thing applies for science storytelling. You can introduce your... -
Plasmids 101: TOPO Cloning
TypeBlog Post...“A” overhang insert, the magic of TOPO cloning happens. Basic procedure Let’s break down the steps needed...1658796. PubMed Central PMCID: PMC52876. 2. Novel approach to molecular cloning and polynucleotide synthesis... -
Donations from Addgene to Yield Answers for Rare Disease Researchers
TypeBlog Post...on OCRL1 already underway in his lab. The gene appears to play a role in many cell processes involving...In Thailand, our annual operating budget is approximately 20,000 USD and we are working on several genetic... -
Don’t Wait To Launch Your Health Science Startup
TypeBlog Post...would apply a percentage of returns to support student projects, but it quickly became apparent that raising... -
Another Pathway into Cells: iTOP
TypeBlog Post...protein-only transduction method and its potential applications to CRISPR/Cas9 genome editing. Protein-only...Diego D'Astolfo. CRISPR/Cas9: a potential application of iTOP Having tested iTOP using Cre-lox recombination... -
Extracurricular Activities for a Strong Science Career Path
TypeBlog Post...multidisciplinary, collaborative, and translational approaches for scientific tool and technique development...leadership, management, and communication. A great approach to fully understand and acquire competitive skill... -
The MTA at Addgene
TypeBlog Post...years! This has helped us develop a fast, agile approach, which we’ve updated and refined as new technology...and our MTA YouTube video for more information. Happy transferring! Many thanks to our Tech Transfer... -
The Importance of High Titer for AAV Transductions
TypeBlog Post...for additional best practices. Keep your cells happy - Make sure your cells are as healthy as can be ...AAV-mediated anterograde transsynaptic tagging: mapping corticocollicular input-defined neural pathways... -
Reagent Repositories Are Speeding up Science During the Pandemic
TypeBlog Post... Another example of reagents that have broad applications but have proven useful to the study of SARS-...et al., 2006). Now that a similar phenomena is appearing with regards to SARS-CoV-2, this mouse strain ... -
Improving Transduction Efficiency with Exosome AAVs
TypeBlog Post...exosomes, small membrane vesicles or microvesicles (approx. 40 - 150 nm diameter) that are secreted from the... This is particularly useful for therapeutic applications in vivo where endogenous anti-AAV antibodies... -
Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids
TypeBlog Post...referred to objects that exist online, but this approach has been adapted for the identification of physical... Addgene plasmid URLs act as unique PIDs, we’re happy to support the RRID and compact identifier formats... -
Celebrate National Book Month with Addgene
TypeBlog Post...science. It's a series of case studies in what happens when scientists bring biases to research and don't...English, I had lapsed. Baker’s novel reignited my appreciation of poetry, and I’ve been reading a poem a day... -
How to Lead a Great Meeting
TypeBlog Post...assign each one using group members’ initials. If appropriate, email this around after the meeting. Accountability...Remember, many of the more in-depth discussions can happen “off-line” with just one or two relevant people... -
Components of CRISPR/Cas9
TypeBlog Post...gRNA) and their prefered Cas9 variant (with the appropriate PAM sequence) to modify the desired genomic locus... more to be found in the full CRISPR 101 eBook. Happy Reading! ... -
Synthetic Photobiology: Optogenetics for E. coli
TypeBlog Post...; only two plasmids are required per system. Applications of these systems The large dynamic range of ...suitable for various scientific or engineering applications. References Refactoring and optimization ... -
Addgene Welcomes John Doench to the Advisory Board
TypeBlog Post...wanted to continue doing research, Doench went on to apply to graduate school at MIT. Doench excitedly remembers...Harlow’s group at Harvard Medical School and then applied to work at the Broad Institute: “The model that... -
Plasmids 101: Mammalian Vectors
TypeBlog Post...should die within 3-5 days and resistant colonies appear in about 10-14 days depending on how quickly your...culture to suppress bacterial growth, and is not appropriate for mammalian selection – do not confuse this... -
Multiplex Genome Editing with CRISPR-Cpf1
TypeBlog Post...methods relied solely on Cas9. Overall, these approaches have two main drawbacks: 1) Most rely on transfection... a simple and effective method with multiple applications (in vitro, in vivo, transfection, viral transduction... -
Addgene’s Viral Service - Why Virus? Why Now?
TypeBlog Post...this need in the community, we approached it in the way Addgene approaches everything; we asked ourselves...