We narrowed to 954 results for: App;
-
TypeBlog Post...multidisciplinary, collaborative, and translational approaches for scientific tool and technique development...leadership, management, and communication. A great approach to fully understand and acquire competitive skill...
-
Celebrate National Book Month with Addgene
TypeBlog Post...science. It's a series of case studies in what happens when scientists bring biases to research and don't...English, I had lapsed. Baker’s novel reignited my appreciation of poetry, and I’ve been reading a poem a day... -
Improving Transduction Efficiency with Exosome AAVs
TypeBlog Post...exosomes, small membrane vesicles or microvesicles (approx. 40 - 150 nm diameter) that are secreted from the... This is particularly useful for therapeutic applications in vivo where endogenous anti-AAV antibodies... -
Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids
TypeBlog Post...referred to objects that exist online, but this approach has been adapted for the identification of physical... Addgene plasmid URLs act as unique PIDs, we’re happy to support the RRID and compact identifier formats... -
Reagent Repositories Are Speeding up Science During the Pandemic
TypeBlog Post... Another example of reagents that have broad applications but have proven useful to the study of SARS-...et al., 2006). Now that a similar phenomena is appearing with regards to SARS-CoV-2, this mouse strain ... -
The MTA at Addgene
TypeBlog Post...years! This has helped us develop a fast, agile approach, which we’ve updated and refined as new technology...and our MTA YouTube video for more information. Happy transferring! Many thanks to our Tech Transfer... -
The Importance of High Titer for AAV Transductions
TypeBlog Post...for additional best practices. Keep your cells happy - Make sure your cells are as healthy as can be ...AAV-mediated anterograde transsynaptic tagging: mapping corticocollicular input-defined neural pathways... -
How to Lead a Great Meeting
TypeBlog Post...assign each one using group members’ initials. If appropriate, email this around after the meeting. Accountability...Remember, many of the more in-depth discussions can happen “off-line” with just one or two relevant people... -
Components of CRISPR/Cas9
TypeBlog Post...gRNA) and their prefered Cas9 variant (with the appropriate PAM sequence) to modify the desired genomic locus... more to be found in the full CRISPR 101 eBook. Happy Reading! ... -
Synthetic Photobiology: Optogenetics for E. coli
TypeBlog Post...; only two plasmids are required per system. Applications of these systems The large dynamic range of ...suitable for various scientific or engineering applications. References Refactoring and optimization ... -
Addgene Welcomes John Doench to the Advisory Board
TypeBlog Post...wanted to continue doing research, Doench went on to apply to graduate school at MIT. Doench excitedly remembers...Harlow’s group at Harvard Medical School and then applied to work at the Broad Institute: “The model that... -
Plasmids 101: Mammalian Vectors
TypeBlog Post...should die within 3-5 days and resistant colonies appear in about 10-14 days depending on how quickly your...culture to suppress bacterial growth, and is not appropriate for mammalian selection – do not confuse this... -
Weighing Reagents Protocol
TypeProtocol...the basics of how to weigh reagents. Wear the appropriate personal protective equipment (PPE) prior to ...contain the reagent while on the balance, and an appropriately sized container to put the reagent in. For some...container. Dispose of the excess material into an appropriate waste container if the material is biohazardous...biohazard solid waste container. Conclusion Appropriately weighing reagents is important to ensure the... -
Multiplex Genome Editing with CRISPR-Cpf1
TypeBlog Post...methods relied solely on Cas9. Overall, these approaches have two main drawbacks: 1) Most rely on transfection... a simple and effective method with multiple applications (in vitro, in vivo, transfection, viral transduction... -
Addgene’s Viral Service - Why Virus? Why Now?
TypeBlog Post...this need in the community, we approached it in the way Addgene approaches everything; we asked ourselves... -
Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors
TypeBlog Post...Juan R. Torruella, U.S. First Circuit Court of Appeals. She served as in-house counsel at St. Elizabeth...responsible for bringing a systematic and disciplined approach to evaluating and improving the effectiveness ... -
Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools
TypeBlog Post...TurboID and miniTurbo podcast segment A fundamental approach to studying membranes, organelles, and other subcellular...directed evolution to create APEX2. They have now applied the same directed evolution technique to the biotin... -
Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems
TypeBlog Post...reactions by mixing this isolated plasmid DNA with appropriate sequencing primers, and hand deliver them to ...standards are met, we are taking a unique hybrid approach compared to our standard pipeline. For the first... -
Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors
TypeBlog Post... SpCas9. In order to expand the gene-editing applications and targeting scope of ABEs David Liu’s lab ...faster deamination rate so that deamination can happen before Cas homologs let go. The Liu lab developed... -
Antibodies 101: Flow Cytometry Controls
TypeBlog Post...ll need a few controls specific to flow as an application. These controls will allow you to distinguish...isotype control. Pro tip! Many companies will have appropriate isotype controls linked on their commercial antibody...