We narrowed to 991 results for: DES

...The natural CRISPR locus of a bacteria host encodes multiple guide RNAs (gRNAs) on a single array to ...applications, we provide detailed manual and online design examples of using Addgene plasmids hCtRNA_FT (#186715...

...if you end up concentrating your AAV above your desired titer and want to dilute it, err on the side of...less buffer than you would to achieve the exact desired concentration to avoid over-diluting, and make ...

...form concatemers in later steps. This new NEB kit includes a step that both fragments the DNA and prevents...of the workshop, NEB gave a brief presentation describing the technology. Then we split into pairs to actually...

...to your community. For those of you who have the desire and ability to, some communities are looking for... results. Also consider using this framework to design your future experiments.  Use Addgene’s AAV Data...

...seeking “public views on emerging IVF techniques designed to prevent the transmission of mitochondrial disease..., and they are not remembered kindly. However, despite many headlines questioning if He’s experiments ...

... determine if proteins are targeted to their destination co- or post-translationally. TRICK: Visualizing... usually translated in a specific compartment. Despite these limitations, these techniques represent powerful...

...references and web links from my first post and start designing your first fly experiment! Many thanks to our...PMID: 28704652. 6. V. Rodríguez, D. Didiano, C. Desplan, Power tools for gene expression and clonal analysis...

... Science. For each publication, Web of Science provides basic bibliographic information and uses its vast...citations due to the journal of publication. Table 1 provides an example calculation of the weights. Table 1...

...speakers from top minds in the stem cell field have described the Yamanaka paper as "life changing". Dr. Yamanaka...affordable. 4. Stem cell scientists are generous Besides some of the scientists I've already mentioned, ...

...mammalian plasmid components, it may be useful to describe the means of introducing genetic material (such...the plasmid in the same way. Selection methods, described below, are typically employed when creating stable...

...expression across the majority of cellular pathways. Despite several years of research, the mechanisms of miRNA...differentiation in a high-throughput manner. They designed the pXPR_053 lentiviral expression vector, which...

...promoter should flank this component as well. On both sides of this promoter-gene combo are two long terminal...Laboratory Press; 1997. Principles of Retroviral Vector Design. Available from: https://www.ncbi.nlm.nih.gov/books...

...through a seminal paper from Peter Schultz’s lab describing a technique that allowed for intentional incorporation...reagents accessible to your lab. The solution GCE provides almost endless possibilities for researchers, ...

...biology – they can deliver materials to specific destinations, haul a variety of cargo, and even handle a ...delivery of the actual virus to which immunity is desired. Instead, components of the virus are now delivered...

...target cells and organisms, and when that cargo is designed to make you sick…well, you feel it. But the same...host genome or temporarily express whatever it encodes. This ability to infect cells with a high efficiency...

... such as citizenship status, academic year, and grades that serve as automated filters of consideration...and cover letter revised by your network, which includes your friends, family members, and professional...

...1x1012 vg/mouse is more standard) and reflects a desire for very high MOI on top of very high coverage....researchers have used the flexibility of AAV genome design in remarkably creative ways. The continued development...

...molecules are recruited by the antibody to help destroy and remove a pathogen. Different isotypes also ...well as the genes that encode those proteins are designated by the lower-case Greek letters μ, δ, γ, ε, and...

... articles reporting chromatin-related research describe the production of plasmids that might be useful...colleagues and guest bloggers have written posts describing new methods and tools for studying chromatin ...

..., the use of LIC is often limited to specially designed plasmids. Sequence- and ligation-independent cloning...the “damage” and producing a complete plasmid. Besides being compatible with any vector, the SLIC protocol...