New Viral Vectors - Fall 2024
Type
Blog Post
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pAAV-EF1a-fDIO-Cre
AAV5
Recombinases
Esteban Engel
New serotype
pAAV-hSyn-Cre-P2A-dTomato
AAV PHP.eB...
We narrowed to 808 results for: Eng;
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Build Your CRISPR Vocabulary
TypeBlog Post... other Cas proteins as well, both natural and engineered. What these proteins all have in common is the... -
Early Career Researcher Toolbox: Free Online Molecular Biology Tools
TypeBlog Post... For general primer design Design primers for Goldengate cloning Design primers for In-Fusion Cloning... -
Five Questions to Ask If You're Deciding Between a Master's or PhD Program
TypeBlog Post...but in time I realized its value. My master’s strengthened my critical thinking and problem solving abilities... -
Viral Vectors 101: Parts of the AAV Packaging Plasmid
TypeBlog Post...Addgene blog: Discover how the PHP.eB capsid was engineered Learn about the parts of the AAV transfer plasmid... -
Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight
TypeBlog Post...switch.” Because of the differing peak absorption wavelengths and light intensity requirements of different... -
Oh, The Places You Can Go: Careers in Science Communication - Curriculum Development
TypeBlog Post...Making science approachable is often the most challenging part of the job, but Caitlin doesn’t work in ... -
Oh, The Places You Can Go: Careers in Science Communication - Science Writing
TypeBlog Post...favorite thing to work on. “It’s one of the most challenging [parts of my job], so making it a success feels... -
Addgene at the ASM Microbe 2016 Conference
TypeBlog Post... Post on CRISPR Methods for Bacterial Genome Engineering Learn how to Network at Scientific Conferences... -
Walking beside Academia and giving it a high five: My transition from graduate work to a position at Addgene
TypeBlog Post...used to spend my days (and often my nights :D) engineering E. coli to produce biofuels in Pamela Silver’... -
10 Ways to Share Your Science!
TypeBlog Post... Consider participating in a future SciFund Challenge. While the goal is ostensibly to get some funding... -
What Do I Do Now? Academic v. Non-Academic Career Decisions
TypeBlog Post...especially with peer mentors, to discuss your strengths, weaknesses, interests and passions is another... -
In Vivo Biotinylation of Bacterial Fusion Proteins
TypeBlog Post...interest fused to maltose binding protein (MBP, New England BioLabs). MBP has a similar function as GST in ... -
Viral Vectors 101: What is a Virus (and a Viral Vector)?
TypeBlog Post...infection, that is. Thankfully, through the use of engineering controls — changes made to the virus that eliminate... -
Antibodies 101: Epitope Tags
TypeBlog Post...doesn’t come with a high background can be a huge challenge. Epitope tags eliminate the need for target-specific... -
What the HEK?
TypeBlog Post...Produce Recombinant Proteins and Viral Vectors. Front Bioeng Biotechnol. (2021) (9):796991. DOI: 10.3389/fbioe... -
Addgene Begins Distribution of Recombinant Antibodies
TypeBlog Post... Gong B, Nguyen K, van der List D, Murray KD, Engebrecht J, Trimmer JS (2019) A toolbox of IgG subclass-switched... -
A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light
TypeBlog Post...MA, Ernst L, Khammash M, Di Ventura B (2021) Engineering AraC to make it responsive to light instead of... -
Viral Vectors 101: Parts of the AAV Transfer Plasmid
TypeBlog Post...sequencing conditions which can read through this challenging sequence. Transgene The transgene is the gene... -
New and Upcoming Viral Vectors - Spring 2019
TypeBlog Post... AAV The GFP-based calcium sensors are single-wavelength sensors with intensities that change in response...