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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 989 results for: IGH@;

Showing: 641 - 660 of 989 results

  1. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    ...protocol, you might have noticed that there are many options to elute your DNA prep. You might see protocols...denature, break, and even change the DNA sequence. High pH favors the breakage of the hydrogen bonds, destabilizing...

  3. CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

    Type
    Blog Post
    ... these results, it’s clear that editing is much higher in the PAM-out configuration. In addition, D10A...D10A editing efficiency is consistently higher than that of H840A, especially with smaller nick distances...cuts close to your insertion site, you can’t obtain high HDR efficiency. Nickases create a staggered cut ...

  4. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

    Type
    Blog Post
    ... Hill. Chromatin immunoprecipitation followed by high-throughput sequencing, ChIP-Seq, is the go-to method... purified for subsequent library preparation and high-throughput sequencing. Improvements to the CUT&RUN...challenging to collect large numbers of cells or when a high degree of signal to noise is desired. It also provides...

  5. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    ... in signaling processes including smell, taste, sight, inflammation, and neurotransmission. But with approximately...activated downstream of GPCRs that are not involved in sight (Olsen RHJ et al., 2020). A transducer complex consists...Gγ-GFP2 will be in close association, resulting in a high level of GFP2 emission. Following activation in ...

  6. Bricking Science: Portraying Scientific Reality Through LEGO

    Type
    Blog Post
    ... LEGO and science: The potential is endless One night, while opening a LEGO set with my husband, I thought...that resembles a real laboratory. It only took one night to build the first model, but because we are [email protected]. Who knows, your story might just get “legofied.” Many thanks to our guest blogger...

  7. Networking and Inspiring Young Scientists at Freshers Fairs

    Type
    Blog Post
    ...Chemistry symposium and a career night. The society also visits high schools to promote chemistry degrees...scientific meetings for students to attend. It also hosts high level academic debates for its members to become...

  8. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

    Type
    Blog Post
    ...arrive. Getting off the plane in Bozeman Sunday night, it was easy to spot meeting participants. They ...the ones holding poster tubes (or as our baffled flight attendant called them, "long, skinny things") and...thinking I was an engineer. The talks we heard Monday night represented engineering first and foremost, from...

  9. Management for Scientists: What Makes a Good Manager Anyway?

    Type
    Blog Post
    ... allow his team to work mostly at home. Having a high degree of freedom and diversity built into their...new things, diversity of responsibilities, which might include training or teaching others, research, and...sometimes taken to an extreme by scientists) Are highly mobile and can move to a new workplace if opportunities...

  10. 10 Ways to Share Your Science!

    Type
    Blog Post
    ...telling it another way. You’ll know you are on the right track if they start asking you questions. Remember...Escape from the Ivory Tower.”  9)   If you think you might want to get serious about science writing, look ...News. Ask your institutional news office if there might be opportunities to blog or write for them. 10)  ...

  11. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    ...bacteriostatic reagents can kill bacteria when used at high concentrations (and vice versa). Looking around ...Antibiotics vary in their sensitivitly to heat and/or light – do not add them to media hotter than about 55oC...55oC and store plates/stocks wrapped in foil if a light-sensitive antibiotic like Tetracycline is used. ...

  12. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    ...the ‘bells and whistles’ – including microscale light emitting diodes, thermal sensors, and microfluidic...channels. These features enable the fibers to emit light for optogenetic purposes, measure temperature, study...construct expressing channelrhodopsin 2 (ChR2), a blue light sensitive cation channel, fused to mCherry to the...

  13. What Do I Do Now? Academic v. Non-Academic Career Decisions

    Type
    Blog Post
    ...surprisingly more rigorous in industrial settings Tight (sometimes impossible) timelines—others are usually... <- Can move between the two (helps to know the right people) -> How can you make a decision about ...interests and passions is another great way to shed light on what paths to pursue. Spend some time really ...

  15. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    ...their secondary structure, palindromic nature, and high GC content. This instability can cause partial loss...through ITRs due to their secondary structure and high GC content, but some companies now offer Sanger ...example, CAG is a strong promoter that drives very high, ubiquitous expression, while the human synapsin...

  16. Meet the New Addgene Board Members!

    Type
    Blog Post
    ...Baradaran says.  Baradaran’s path to science began in high school. “I had a great chemistry teacher in 10th...medicines can have on human health. As a teenager in high school, I knew that this is what I wanted to do,...ClonTracer Barcoding Library, a library that “enables the high-resolution tracking of more than 1 million cancer...

  17. Celebrate Open Data Day with Addgene’s AAV Data Hub!

    Type
    Blog Post
    ...and it is under copyright, you will need to obtain written approval from the copyright owner and will ...treatment conditions like drugs (Chemogenetics) or light (Optogenetics) to activate receptors? If you’re ...

  19. CRISPR Challenges: Standardization and Homology Directed Repair

    Type
    Blog Post
    ...Delivery The RNP delivery method consistently leads to high on-target editing and low off-target editing rates...SpCas9 The closer your edit is to the PAM site, the higher the efficiency of HDR repair. Check out this post...post to see if an engineered Cas9 variant might work for your experiment. PITChing MMEJ as an Alternative...

  20. 5 Uncommon Ways to Gain Career Skills During an Uncommon Time

    Type
    Blog Post
    ...forward. Here are a few out-of-the-box ideas that might jump start your learning. 1. Create a peer mentoring...what’s a scientist to do with no live audience in sight? You could do a talk virtually for your peer mentoring...university or use your current contacts to ask who they might suggest you speak with. See this post that’s all...
Showing: 641 - 660 of 989 results