We narrowed to 934 results for: INA
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TypeBlog Post...insert. Example, if you have a Flag tag on the N-terminal of your insert, you would list it first. pBACKBONE-Flag-hGene...pBACKBONE-Flag-hGene If there was also an EGFP fused to the C-terminal of your insert you would list it after the insert...
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Addgene Stands with the Scientific Community
TypeBlog Post...otherwise be lost amid lab closures or project terminations, ensuring that scientific progress made through... are constrained. This collaborative approach eliminates redundant work, saving time and allowing precious... -
2022 and 2023: Reflections and Looking Forward
TypeBlog Post... the launch of our fast-growing ready-to-use recombinant antibodies collection, currently composed of ...the community to drive new science experiments. Finally, we were thrilled by how many people accessed our... -
Your Top Requested Plasmid in 2015!
TypeBlog Post...2015 was... lentiCRISPR V2 LentiCRISPR v2 originates from Feng Zhang’s lab at the Broad Institute ...cells, deciphering the mechanism of matrix metalloproteinase-9 secretion, and identifying lysine methyltransferase... -
Save Time and Money by Making Your Own Competent Cells
TypeBlog Post...adding plasmid) to make sure you don’t have contamination. Also conduct a positive control (test a ... Melina Fan... -
Q&A With Julie Cicalese, the Newest Member of the Addgene Board of Directors
TypeBlog Post...forward. Sooner or later enough people will be vaccinated and we’ll have some herd immunity, which raises...Giving think tank event where we brought a multidisciplinary group of scientists together to draft a research... -
CRISPR-Cas14: a family of small DNA-targeting enzymes enabling high-fidelity SNP genotyping
TypeBlog Post...cleavage, the authors showed that Cas14 could indiscriminately cleave ssDNA in trans like Cas13 and Cas12...adding Cas14 to the mix, you get the ultimate combination enabling the detection of RNA, dsDNA, and now... -
Science Communication Snapshot: DayCon 2015
TypeBlog Post... timely scientific topics, as well as archived seminar presentations. One of SITN's newest initiatives...The afternoon program mixed things up with a combination of shorter, more narrowly focused lectures, interactive... -
Designer PUF Proteins for Any RNA Target
TypeBlog Post...of the high fidelity version (despite what the original paper says). In their hands, BsaI-HF has produced...they hope others will do the same. “This isn’t the final version,” she said. “If we learn how to make the... -
Editor's Choice, August 2016
TypeBlog Post...technique uses catalytically inactive CRISPR endonuclease (dCas9) fused to a deaminase to make the transitions... -
Modulate the Activity of 17 Signaling Pathways with One Kit!
TypeBlog Post..., in collaboration with “cloning guru” Kathleen Ottina and David Sabatini at the Whitehead Institute and... over-expressed, constitutively activated or inactivated the pathway. “The idea is that if you introduce... -
X-CHIME: Context Dependent Germline Knockout in Immune Cells
TypeBlog Post...different cell types, at different times, and in combination with different genes. Having CRISPR systems for...G., & Sharpe, A. H. (2024). X-CHIME enables combinatorial, inducible, lineage-specific and sequential ... -
Searchable and Sortable gRNAs for Your Next CRISPR Experiment
TypeBlog Post...that you confirm the details and final experimental outcome in the original publication. As you develop and... -
Addgene is Expanding Our Viral Vector Service!
TypeBlog Post...or in vivo testing. We recommend you check the original paper of your AAV plasmid of choice for more information...be available through our distributor partners in China, Brazil, Japan, Korea, Mexico, and India. Once the... -
Spurred by COVID-19, Addgene Bridges Plasmid Sharing Between Academia and Industry
TypeBlog Post... more broadly to accelerate COVID-19 research: Sinai Health System ...Essen University of North Carolina at Chapel Hill University of Illinois Chicago ... -
Increasing Your Work's Visibility with Addgene: Citation, Search, and Collections
TypeBlog Post...Addgene ID number as well as the reference for its original publication. To help facilitate this process, ...also increases the visibility of both you and the original depositor. Our citations feature makes it so that... -
A Better Way to Get Customer Support: The Help Center
TypeBlog Post...philosophy Customer service can be a hassle. You finally make it through a 20-step phone tree, only to be... be left on hold for an hour. When you finally speak to a human being, you may not even get a solution... -
Mapping the 4D nucleome with CRISPR/Cas9
TypeBlog Post...are thus limited by its lower resolution. The combination of this method with superresolution microscopy... is so high, cells may be imaged under lower illumination settings, lowering photobleaching and phototoxicity... -
9 Tips to Achieve Success in Academia
TypeBlog Post...than intensely competitive. Be curious and interdisciplinary. 3. “There are many different ways to succeed...science, especially if the conference is a big multidisciplinary one.” Cepko strongly emphasizes that people... -
Fluorescent Proteins 101: Fluorescent Biosensors
TypeBlog Post...fused to calmodulin (CaM) and the chicken myosin kinase calmodulin binding region RS20. When calcium is...for single and multi-color imaging experiments. Finally, what kind of system will you use to detect your...