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We narrowed to 1,076 results for: IND

Showing: 641 - 660 of 1076 results

  1. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    ...information for postdoctoral positions is very hard to find and this is partly due to the administration of ...paid. Institutional salary policies are one indicatator of how salaries may changes due to the FLSA. ...Current salary information that we have been able to find suggests that salaries may even double. The University...to the new level:  We are still waiting for an indication of what (most of) Harvard, Johns Hopkins, (most...make gradual adjustments towards this new level. Indeed some institutions did this. It doubtless took a...

  2. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    ...committee to understand your goal, what you expect to find, and how you will interpret and use the information... correct or your data favor a different model. Finding evidence for or against a model is a standard scientific...knowledge gap? This includes the overview and the individual aims. Describe the question, the bare minimum...know they will work. Stabilize your blood sugar. Find an energy bar that you like and consider eating ...photographer.  Additional resources on the Addgene blog Find tips for presenting a science talk Check out our...

  3. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    ... everyone. Addgene is certainly going to have to find ways to tag and curate reagents from these emerging...emerging organisms so scientists can easily find the useful new reagents that come out of this work as the...parenting strategies and make important ecological indicators since species live in water and on land. Current...nests so can be studied over time. They recognize individual faces (I guess of other wasps) so can be used... expensive. They are using a cool fluorescent indicator system to rapidly identify recombinants. Arthropods...

  4. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ...synthesis relies on transfer RNAs (tRNAs). tRNAs bind to and deliver amino acids to the ribosome where...complementary to the mRNA sequence. The 3’ end of  the tRNA binds the amino acid that corresponds to the anticodon...1980s, scientists even created a codon adaptation index (CAI) that is based on the codon usage frequency...https://doi.org/10.1038/nsmb1031 Gustafsson C, Govindarajan S, Minshull J (2004) Codon bias and heterologous...nrg3051 Sharp PM, Li W-H (1987) The codon adaptation index-a measure of directional synonymous codon usage ...

  5. The Challenges of Cell Culture

    Type
    Blog Post
    ...Proliferation and Invasion in Renal Cancer Cells, Marker-independent identification of glioma-initiating cells. The...that can impede collaborations, especially with industry. Today we also have the appropriate tools to verify... standards and has laboratories to carry our independent quality control for a range of testing, from ...forensics, DNA sequencing to drug testing. You can find Nick on LinkedIn. References 1. Skloot, Rebecca,... Protein Localization Resources on Addgene.org Find Plasmids for Mammalian Cells ...

  6. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ...other important binding events take place during the course of infection. These binding events can be impaired...infect and select, seems simple, in actuality, many find using lentivirus to be time consuming, difficult...possible. One problem that often plagues labs and hinders experiments is mycoplasma contamination. Mycoplasma...Lentiviral Vectors Additional Resources on Addgene.org Find Viral Vectors for Your Research Brush Up on All ...

  7. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    ...nose-poke at a particular hole (the active hole) induces laser stimulation of the chosen brain circuit, .... If activation of the circuit is sufficient to induce reinforcement (instills a sense of “reward”), then...software such as EthoVision. Consult the literature to find all the applications of optogenetics relevant to...region may affect the animal; stimulation could induce seizures or some other unwanted phenomenon or it...learn optogenetics is straight through the source - find a lab within your own neuroscience sub-discipline...

  8. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    ...hero (that I’m not), FRET loves playing “live.” Indeed, FRET was one of the first techniques which enabled...Historically, molecular interactions were detected by indirect means often using probes with the potential to...may have to design several different constructs to find one that could be used for the purpose of your studies.... But it is a parameter that you have to keep in mind when analysing FRET results. The cross-talk between...also don’t have any resonance transfer. You have to find a balance between FRET efficiency and cross-talk...

  9. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    ...University of Washington reported a surprising finding - when delivered via AAV infection, gene targeting...efficiency gene targeting approaches improved on this finding with the addition of selectable markers that are... et al 2004, Kim et al 2008). While the biology behind this AAV effect remains unclear, it is likely not...infect recipient cells. After G418 selection, individual gene edited clones are identified by PCR. Then...Resources on Addgene.org Check out our Viral Service Find AAV Vectors Browse all Viral Vectors ...

  10. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    ... Joanne's 10 Things Scientists Ask about Finding an Industry Job Talk What’s Your Story? By Herminia Ibarra...speaking early and often. Two of my jobs have indirectly resulted from someone seeing me speak in a non-interview...before tailoring each presentation appropriately.  Find out who will be listening.  Are they early, mid,...explanation. The people that need the information will find it. Experienced speakers present useful protocols...

  11. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    ... of E. coli used today are descended from two individual isolates, the K-12 strain and the B strain. K...used in the lab Most of the commercial strains you find today are marketed for a specific purpose: fast ...conjugation. Genes listed on F´ are wild-type unless indicated otherwise fhuA (formerly tonA) ferric hydroxamate...typically standard; just having the gene listed indicates it is non-functional. If a gene is deleted, it...Vectors Looking for More Plasmids 101 Articles?  Find them all at our Plasmids 101 Topic Page!     ...

  12. Introducing the Addgene Antibodies 101 eBook!

    Type
    Blog Post
    ...’t quite seem to find an antibody that works, we highly recommend “Chapter 3: Finding the Right Antibody...very new to antibodies as research tools, you may find “Chapter 1: What is an Antibody” quite useful. If...place to start looking for answers.    We hope you find the latest addition to our eBook collection useful...

  13. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    ...for your experiment? Use Addgene’s AAV Data Hub to find experimental reports on commonly used vector cargo...Serotype 4 (AAV4) and AAV5 Both Require Sialic Acid Binding for Hemagglutination and Efficient Transduction...Viswanathan, S., Gaj, T., Lavzin, M., Ritola, K. D., Lindo, S., Michael, S., Kuleshova, E., Ojala, D., Huang..., M. J., Chiorini, J. A., & Zabner, J. (2001). Binding of adeno-associated virus type 5 to 2,3-linked ...α2,6 N-Linked Sialic Acids Facilitate Efficient Binding and Transduction by Adeno-Associated Virus Types...

  14. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    ...Addgene.org Check out our CRISPR Guide Find Plasmids for Cas9 Purification Find CRISPR Plasmids for Mammalian ...including one loxP site insertion and one NHEJ-repaired indel, often occur. Figure 1: In conventional gene...recombinases (Cre, Flp etc.), and tetracycline drug induction systems (ttA or rtTA) are between 1 to 2 kb in...al. saw success with Easi-CRISPR across three independent core facilities for 13 projects, with most projects...

  15. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ...calcium binding protein) fused to two fluorescent proteins. When the responsive protein binds to calcium...Venus fluorescent protein, the Troponin C Ca++ binding domain, and NanoLuc® Luciferase. CalFlux VNT responds...2017) Genetically encoded bioluminescent voltage indicator for multi-purpose use in wide range of bioimaging...Plasmids Browse the Fluorescent Protein Collection Find Fluorescent Protein Biosensors ...

  17. 5 Science Rap Videos & Their Creator: Tom McFadden

    Type
    Blog Post
    ...Science rap mastermind, Tom McFadden, recently worked with high school students in the bay area to create...listen to the interview with Tom McFadden!   1. Rosalind Franklin vs. Watson & Crick - Science History ...story telling following the unfortunate story of Rosalind Franklin and her incredibly important role in ... Student Community Check out All of Our Podcasts Find Great Educational Content in our Plasmids 101 Series...

  18. 2018 #DeckTheLab Contest Winners

    Type
    Blog Post
    ...red nosed reindeer.       View this post on Instagram     Eppi the red nosed Reindeer! 🦌🦌 as you...nikaheijmans) December 21, 2018 Eppi the red nosed reindeer from @ladiesinlabcoats Many teams recycled lab...ladiesinlabcoats #Christmas #labchristmas #cute #reindeer #DeckTheLab #eppendorf #themuppets #scifun #funny...

  19. Providing Plasmids to Researchers in Developing Countries: Addgene and Seeding Labs Team Up

    Type
    Blog Post
    ...Researchers in developing countries often find themselves on the front lines of emerging global challenges...and Africa, but we need to expand our efforts to find new and creative ways to reach developing countries..., as well as shine a light on some of the great minds working in developing countries. As the world continues...and organizations like it. Additional Resources Find out how you can get involved with Seeding Labs Follow...

  20. The Developmental Studies Hybridoma Bank: Over 25 Years of Antibody Sharing

    Type
    Blog Post
    ...widely used class of protein-binding reagents. In the lab, their binding specificity allows scientists.... The ends of the branches contain the antigen binding sites and are highly variable, allowing exquisite...The immortalized cells, called hybridomas, are individually isolated and expanded. The clonal cells are ...
Showing: 641 - 660 of 1076 results