We narrowed to 916 results for: IRE;

1. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

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   ...limited deep-tissue performance, and sometimes require an alternative such as a radioactive tracer. As...cell differentiation. Furthermore, TALEN and ZFN require custom nucleases for each gene of interest. CRISPR-Cas9...

2. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

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   ...DNA base changes and consist of a catalytically impaired Cas protein (dCas or Cas nickase) fused to a DNA-modifying...editing target site on the DNA, the Cas domain directs the modifying enzyme to the target site, and the...

3. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

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   ...the research is ongoing, but all registries do require that research plans become public eventually). ...implement authentic scientific research and as the Director of Advising in the Division of Life Sciences at...

4. Building and Validating Recombinant Antibodies: A Case Study

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   ...to WGS the lab’s entire hybridoma collection. Instead, the Trimmer Lab moved to direct functional validation...

5. Antibodies 101: Isotypes

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   ...response, the variable region of an antibody is paired with any of the other isotype heavy chain constant...deleted.     Figure 2: Isotype switching requires DNA recombination of the antibody gene locus. ...

6. GPCRs: How Do They Work and How Do We Study Them?

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   ...new ligands for unknown GPCRs. You can find the entire PRESTO-TANGO kit at Addgene! TRUPATH: Bryan Roth...chemogenetics receptors, GPCRs were first mutated via site-directed mutagenesis to bind non natural ligands. These...

7. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

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   ...antibodies to recognize and bind transcription factors directly, but that’s a slow-going process and it doesn’...transcription factors. The second vector is a homology directed repair donor plasmid with homology arms and a ...

8. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

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   ...screens that can be done with the libraries will require access to next generation sequencing technology...due to the size of the backbone, transformations require electroporation. References & Further Reading...

9. Human Germline Editing Using CRISPR

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   ...targeting efficiency of only 27.9% and homology-directed repair (HDR) taking place in 41.2% of edited cells...NHEJ rather than HDR. Figure 1: Homology-directed repair in targeted zygotes proceeds from the maternal...

10. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

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    ...peptide derived from cytochrome p450, CytERM, to direct the fluorescent protein to the Endoplasmic Reticulum...Plasmids for Subcellular Localization Browse the entire Fluorescent Protein Collection ...

11. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

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    ...of ccdB, leading to positive identification of desired clones.   ...Becomes a powerful cloning tool... efficiently, as those that do not contain the desired insert should not grow.   Figure 2: Image...

12. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

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    ...pyogenes Cas9, dCas9-effector fusions, and xCas9, and requires no additional chemicals or modifications. This...optogenetic tools can be difficult because this requires detailed structural knowledge of the proteins....

13. Reproducibility for Everyone: Lessons from an Open Science Collaboration

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    ...thinking about reproducibility and shared similar desires to take action. Thanks to Teytelman’s vast network...already run several reproducibility workshops as Director of Scientific Outreach at Code Ocean and through...

14. Antibodies 101: Affinity Reagents

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    ...available for a large number of targets, and can require specific conjugates. And, of course, once you have...either re-develop or re-optimize your assay for an entirely new affinity reagent.    Fig. 1: A comparison...

15. Transferable Skills Guide: Problem Solving

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    ...and the lab, you may not be able to carry out the entire problem solving process on your own, but you can...skillset is a part of your toolkit is important. Paired with your other skills, both technical and transferable...

16. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

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    ...BioRender.com. Image from Haoyi Qiu.   He Tian: Directed evolution of biosensors He Tian (goes by “Tian...developed a video-based high-throughput screen for directed evolution of biosensors based on their dynamic...

17. Summer SciComm: Preprints

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    ...scientists can receive recognition for their efforts, inspire future research, and increase their impact. Open...’ comments, and author responses can be shared directly alongside the preprint while the work is still...

18. Seven Tips for Using LinkedIn as a Scientist

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    ...those planning a career in academia, it may not be required, but many academic scientists are starting to ...use LinkedIn messaging, not everyone likes to. A direct email can be the best way to get in touch if you...

19. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

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    ...ability to turn on genes at different times. It requires more complex synthetic networks. Addgene: What... with the software to tell you which parts are required to get the function that you want. The last few...

20. Design Tips for Prime Editing

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    ...reverse transcriptase (RT) template including the desired edit. Figure 1: Prime Editing schematic....guide Find CRISPR plasmids by function Browse our entire plasmid collection ...