We narrowed to 1,035 results for: LAS
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TypeBlog Post...strain used). Similar to the goodwill of Addgene plasmid depositors, this growing resource of over 100 entries...expression after AAV injection, what region and cell class ended up expressing your virus? If you were using...
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10 Great Guest Posts We're Thankful For
TypeBlog Post...Nathaniel Roquet from the Lu Lab at MIT developed plasmid based systems that report the order in which specific... plasmid... -
Using Addgene's New Viral Service for Your Research
TypeBlog Post...accelerate your research. Why distribute virus? Plasmid distribution is booming, but, over the years, many...list of what’s available or suggest additional plasmids with the Viral Service Suggestion Form. Lentivirus... -
Summer SciComm Series: Master of SciComm
TypeBlog Post...course involved a whirlwind of skill acquisition. Classes covered filmmaking, podcasting, philosophy of science... Ramanujan, to be precise) and remarking to a classmate that, if this was one day required of me for work... -
Intro to Our Summer SciComm Series!
TypeBlog Post... through a structured environment such as the classroom or a defined learning program. Learners cannot...high school who are also students in AP biology classes Creating a podcast about genetics using dog ... -
Extracurricular Activities for a Strong Science Career Path
TypeBlog Post...Extracurricular Activities Professional Skills Open source classes (e.g. edX, MIT OpenCourseWare) Access to knowledge... (e.g. Volunteer at a local high school science class: Science Club for Girls) Communication to diverse... -
Celebrate National Book Month with Addgene
TypeBlog Post...poetry, and I’ve been reading a poem a day for the last 8 years. Since then, we’ve read books across nearly..., true crime, and so on. We’ve read well-known classics, like Austen’s Pride and Prejudice, and some more... -
The Crispr Babies Saga Shows the Need for Action, Not More Delays
TypeBlog Post... are many things we can navel-gaze over from the last couple of weeks, including whether or not scientists...Addgene.org Read our CRISPR guide Find CRISPR plasmids by function Check out our genome engineering guide... -
Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**
TypeBlog Post...the MG-2I-dL5** FAP-TAP system works. Find the Plasmids from the Publication Here Light-induced cell ablation...Vitriol, E., & Hahn, K. (2008). Chromophore-assisted laser inactivation in cell biology. Trends in Cell Biology... -
Learning Technical Skills at a Lab Central Workshop
TypeBlog Post...Library Prep Kit for Illumina sequencing and took a class at NEB’s new teaching space at Lab Central to learn...drawstring backpack and a double wall insulated glass to take home with us. Tips to to get the most out... -
DAP arrays for multiplex gene editing
TypeBlog Post...tRNA-gRNA arrays, which is scalable and efficient in plasmid and viral vectors. In applications when repetitive...manual and online design examples of using Addgene plasmids hCtRNA_FT (#186715) and hCtRNA_VT (#186716). ... -
New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor
TypeBlog Post... between two halves of a bacterially derived periplasmic glutamate binding protein known as GltI. When...virus aliquots being deposited at Addgene. Other plasmids and pAAVs can be ordered directly from Addgene... -
Optimizing Donor DNA for Enhanced CRISPR Genome Editing
TypeBlog Post...validated editing reagents can be found on our plasmid pages. We encourage any labs performing genome ...CRISPR Background with Our CRISPR Guide Find CRISPR Plasmids for Your Research Check out Our List of gRNA Design... -
Choosing Your Fluorescent Proteins for Multi-Color Imaging
TypeBlog Post...spectra of your protein with the filter sets or lasers on your microscope. Ideally, you would like to ...Rhodamine / Cy5 (e.g. Semrock's) and the similar 4-laser combination on a confocal (405 / 488 / 561 / 640... -
Antibodies 101: Monoclonal Antibodies
TypeBlog Post... can clone the genes from the hybridoma into a plasmid to create a recombinant antibody. Figure...Resource on Addgene.org Check out Addgene’s antibody plasmid collection Read the latest on Addgene's new antibody... -
7 Tips to Secure a STEAM Internship This Summer
TypeBlog Post...open doors to a web of opportunities that the classroom cannot offer. Furthermore, companies across most... letter that is customized for each internship. Lastly, have your resume and cover letter revised by your... -
Antibodies 101: Flow Cytometry Controls
TypeBlog Post...be from the same host species, have the same Ig subclass, and be in the same fluorophore (e.g., if you ...staining for anti-mouse Ly6C in APC with the IgG2c subclass, you will use the APC IgG2c isotype control). ... -
Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity
TypeBlog Post...Visit our CRISPR plasmids and resources page Read our CRISPR guide Find CRISPR plasmids for mammalian systems... -
Dos & Don'ts When Publishing a Scientific Manuscript
TypeBlog Post...manufacturers’ details for antibodies, plasmids (like your Addgene plasmid #), and other reagents. F1000Research... -
Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics
TypeBlog Post...structures that determine membrane expression and plasma membrane localization of transmembrane proteins...the VTA is located in the midbrain, fiber-optic cannulas were still stereotaxically implanted into the ...