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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 921 results for: Por

Showing: 641 - 660 of 921 results

  1. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    ...sequence flexibility indicates that gRNAs could be incorporated into these constructs without compromising cleavage...slightly higher mutation rates than they’d previously reported in protoplasts (15% and 9% of target sites.) How...

  2. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    ...process involve repairing chromosomal DSBs. It is important to know that cells possess two major repair pathways...chance of causing a frameshift mutation. Of some importance, the deletion can be less heterogeneous when ...

  3. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    ...): These are considered low copy vectors and incorporate part of an ARS along with part of a centromere...nutrient, the host cells will die unless they have incorporated the plasmid carrying the required gene.  The...

  4. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    ...plasmids have been introduced by transfection or electroporation. All you do to create a protein that binds ...where particular variants or mutations have been reported in people, we can use this kit to knock in those...

  5. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    ...for IgG subtypes from different animals so it’s important to choose the reagent that works for your species...sure the staining you are seeing is real. It’s important to include a negative control using serum from...

  8. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    ...interacting proteins, which results in poor spatiotemporal resolution for in vivo work. APEX2, a faster...identify highly biotinylated background proteins reported by other research groups that performed BirA*-...

  9. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    ...combinatorial libraries, and high specificity has been reported for a number of targets. Due to their size and...ain't broke, don't fix it!  That being said, it's important to know all your options when planning and running...

  10. To Each HIS Own

    Type
    Blog Post
    ...tended to be very large and often required the incorporation of a cleavage site and a post-purification cleavage...very pure sample. Because binding strength is proportional to the length of the tag, with longer tags you...

  13. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    ...Thesis (who has also blogged for us) offers a free report on her website called “Secrets to Success in Graduate...be helpful having a coach that can offer extra support for issues of diversity in science.  A great person...

  16. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    ...about publications in the fields of research they support. Monitoring these types of accounts can help you...and figures using machine learning to extract important features such as influential citations, images...

  19. Hot Plasmids - October 2020

    Type
    Blog Post
    ...grown in high concentrations of IL-3 show a high proportion of blue fluorescence, indicating cell proliferation...they could not proliferate. Eastman et al. Cell Reports 2020. https://doi.org/10.1016/j.celrep.2020.107804...

  20. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    ...antibodies for IHC. Working with collaborators and support from the Brain Research Through Advancing Innovative...recombinant antibodies are a newer technology, it is important to show through qualitative data that they are...
Showing: 641 - 660 of 921 results