We narrowed to 1,006 results for: RING;

...successful inhibition of hippocampal neurons. Initial monitoring Second, this may sound rudimentary, but before...exciting—and unanticipated—road of discovery. Monitoring behavioral output The appropriate behavioral ...science writing. Derek is also a pianist and an aspiring composer. View his blog drsimonsaysscience.org...

...The first time I heard about FRET during a journal club, my guitarist brain automatically thought about... recently and is the most rigorous method for measuring FRET. FLIM measures the fluorescence decay time...MW. Fluorescent proteins for FRET microscopy: monitoring protein interactions in living cells. Bioessays...

...sequence in the way that they like, the design and engineering of fusion proteins is relatively straightforward...assessing oligomerization in cells. In addition to measuring stickiness, several researchers have also collected..., Marieke, et al. "A FRET-based biosensor for measuring Gα13 activation in single cells." PloS one 13.3...

...  Communication (or lack thereof): Information sharing gets more challenging the bigger an organization... can help, and if and when you find yourself transferring this skill to another job, it will be important...conflicts is not inevitable. When you know you are bringing this asset to your job, you can become a valuable...

...Immunofluorescence (IF), is an immunoassay that brings to light the cellular world. The technique allows...poorly in a given context, let your community know! Sharing your experience, positive or negative, will help...Immunofluorescence Staining. In: Methods in Molecular Biology. Springer New York, pp 299–311. https://doi.org/10.1007/...

...need to test for this in your system if you’re measuring expression over a period of weeks (in vitro) or...to turn on, or turn off, gene expression by administering an inducing agent like doxycycline (Favre et...cells you want to deliver to, and how you’ll be measuring your experimental output. You’ll also want to ...

...Hippocampus labeled with anti-Kv2.1 (magenta), anti-AnkyrinG (green), and Hoechst nuclear stain (cyan). Panels...expect the target to be. You can test this by preparing a sample without the primary antibody, or with...Yong (Ed.), Biobanking (Vol. 1897, pp. 299–311). Springer New York. https://doi.org/10.1007/978-1-4939-8935...

...make lentiviral vectors fantastic options for delivering shRNA, CRISPR/Cas9 components, and fluorescent...lines but reach their limits when it comes to transferring DNA into primary cells or non-dividing cells...virus. Even though this system was efficient at transferring the gene of interest to the appropriate cells...

...Matthew L., Sonya J. Wolf, and R. J. Samulski. "Delivering transgenic DNA exceeding the carrying capacity...comparative study of strains of Halobacterium halobium differing in pigmentation." Biochemical and biophysical ...Schnütgen, Frank, et al. "A directional strategy for monitoring Cre-mediated recombination at the cellular level...

...pipetting! We’ll release a new tip every two weeks during the school year. Our first quick tip, “Your Bench...  Want your quick tips even quicker? We’ll be sharing our favorite parts of every quick tip video on ...

...collection of memorabilia, even made an appearance during co-founder and CSO Melina Fan's words of welcome...you all so much for an amazing twenty years of sharing science!       ...

... What a fun video of Mario, dressed as Santa, delivering gifts to the Mushroom Kingdom. Kravitz Lab 2019...snowflakes, ornaments, lights, and took a group photo wearing holiday sweaters and Santa hats. There was even...

...Bacterial Expression Systems. Check out our Genome Engineering Overview and our CRISPR guide to find out how...how CRISPR is being used for bacterial genome engineering. To make it easier for you to find plasmids ...

...plasmid-based technologies. Commentary about scientific sharing and the open-access movement. At Addgene, we believe...the conversation! Do you have a question about culturing iPS cells? Confused by which system to use for...

...and to get some insight into her management and mentoring styles. If you'd like to learn more about Professor...defect in a single gene) 26:00-28:39 : Methods for Delivering Multiple Genes to Cells for Gene Therapy 28:40...

...experiences as well as plasmids from Addgene to bring new opportunities to Ghanaian scientists Click ...synthetic biology, DNA assembly methods, and engineering cellulose degradation. 18:21 - 23:26 : Kwabena's...

...packaged on request, you’ll see a banner below the ordering table. Click on it, select your desired serotype...find this service useful in your research. Happy vectoring! ...

...standardized way. Since Addgene encourages the public sharing of aggregated data only, information on requesting...you find the dashboard useful when analyzing and sharing your own depositor metrics!  About the Author Joachim...

...vectors repository since March.  Addgene is now offering an AAV2 CAG-driven tTA expresser from the Gradinaru...PdCO, deposited by the Yizhar lab. Addgene is now offering AAV1 and AAV5 preparations of several variants...

...direct impact on human health and look forward to hearing from her in the future. Future awardees We will...to learn more about future awardees and their inspiring research. Additional Resources on the Addgene...