We narrowed to 728 results for: STO;

1. Plasmids 101: Broad Host Range Plasmids

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   Blog Post

   ...properties that influence host range are not fully understood, but scientists have identified some of the features...

2. Transferable Skills Guide: External Collaborations

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   Blog Post

   ...keep it short. Soon, discussing with strangers stopped feeling weird and started to be fun. I just had...

3. Plasmids 101: Gateway Cloning

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   Blog Post

   ...elements (ribosome recognition sequences, start codon, stop codons, reading frame considerations, etc). This...

4. Using Video to Share Your Science: We Share Science

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   Blog Post

   ...is not hard either. Here are some resources: ImpactStory, a website that helps researchers go beyond citation...

5. Changing Labor Laws Bring Increased Postdoc Wages

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   Blog Post

   ... all already have salaries at or above $50,000. Boston Children’s Hospital is the only part of the Harvard...

6. CRISPR Activation: A Practical Guide

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   Blog Post

   ...plastic tool and has taken the scientific world by storm. While Cas9 has been most widely used to create ...

7. Important Considerations in Optogenetics Behavioral Experiments

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   Blog Post

   ...reinforcement are ICSS and place preference.   Historically, ICSS was done by implanting an electrode and...

8. Plasmids 101: E. coli Strains for Protein Expression

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   Blog Post

   ...purification protocols are designed for soluble, cystosolic protein products, but this is not always achievable...

9. Lentiviral Vector Guide

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   Guide

   ...VSV-G due to wide infectivity) VSV-G (vesicular stomatitis virus G protein) is the most common envelope ...supernatant containing the viral vectors is removed and stored or centrifuged to concentrate. You can use crude...line protocol . Browse Addgene's collection of in-stock lentiviral preps . CRISPR Genomic Screens Genome-wide...on addgene.org Lentiviral Plasmids Collection In-Stock Lentiviral Preps Addgene's Viral Prep Service Viral... The viral envelope gene; typically vesicular stomatitis virus G glycoprotein (VSV-G), an envelope protein...

10. CRISPR 101: Homology Directed Repair

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    Blog Post

    ...single-stranded DNA templates. Jacobi SA, et al. Poster at Keystone Precision Genome Engineering. 2017.  Easi-CRISPR...

11. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

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    Blog Post

    ...envelope plasmid typically encodes the vesicular stomatitis virus G protein (VSV-G), which facilitates viral...

12. Antibodies 101: Introduction to Immunofluorescence

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    Blog Post

    ...with IF are immunocytochemistry (ICC) or immunohistochemistry (IHC). All three of these terms refer to...

13. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

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    Blog Post

    ... a split BirA* molecule that can reassemble to restore activity when fused to two interacting proteins...

14. Harnessing Bacterial Toxins for Allelic Exchange

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    Blog Post

    ...postdoctoral researcher at Harvard. There’s an astounding number of ways to create chromosomal mutations...

15. Hot Biosensors 2022: Year-End Roundup

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    Blog Post

    ...10.1021/acssensors.1c02201. LiLac sensor for lactate Stop and smell the flowers - or rather, the lactate! ...

16. Adeno-associated Viruses (AAVs) for Genome Editing

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    Blog Post

    ...Although the mechanism of incorporation is not well understood, researchers have been using AAV vectors for ...

17. Transferable Skills Guide: Time Management

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    Blog Post

    ...final read Browse list of previous depositors from Boston University Contact Dr. Gilmore directly to see ...

18. New Optogenetic Tools for Cytoskeleton and Membrane Control

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    Blog Post

    ...GTPase optogenetic systems (Berlew et al., 2022). Stopping traffic with CIB1-Rabs Instead of using an optogenetic...

19. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

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    Blog Post

    ...CRISPR has taken the genome engineering world by storm owing to its ease of use and utility in a wide variety...

20. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

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    Blog Post

    ...) to name two more. Likewise, there are several store-bought cloning kits available which are all based...