...isolation of successful transformants. Addgene distributes several ready-to-use, modular plasmids, combining...combining fluorescent tags, epitope tags, protease sites, and selection markers. These are especially useful...

...gives a presentation on what they do and how it relates to other teams at the company. This provides new...old) employees with information on how Addgene operates as a team and shows how they fit into the system...

...research by anyone. As infection rates were increasing in the United States, and many universities and Technology...

...pyogenes system does not work well in Caulobacter). To test their CRISPRi system, the Laub lab repressed ctrA...that the levels of ctrA mRNA decreased after 20 minutes in xylose (which controls expression of dCas9)....

...injection site. For an overview of AAV administration routes for neuroscience see Table 1 from the paper. 2....practical guide by the workshop participants  - a testament to openness and sharing in science.  We’re excited...

... to its modular structure. Two multiple cloning sites (MCS's) flank the M24 promoter, allowing it to be... customizing gene expression. Sahoo et al. have tested the system with genes up to 4 kb in length and ...

..., or how organization may be altered in disease states. dCas9 gets a colorful makeover Even without its... cross-talk with the other orthologs. Ma et al. tested their dCas9 variants using gRNAs specific for telomeric...

...a blank white slate and developed this over 30 minutes, which was fun to watch. A simpler 1 protein -... is already modifying cell and animal genomes to test hypotheses of disease. They are finding they have...

...until now, you’ve always had to call the United States - Cambridge, MA to be exact. Today, that changes...expression of an unknown protein in macrophages/monocytes using lentiviral expression vectors. I also used...

...of our protocols supports reproducibility and accelerates science. Here, we list the specific equipment...instead of room temperature or 37 °C. The protocol notes when there are different incubation options available...empirically determined. If possible, run an initial test with serially diluted primary antibody and determine...Equipment Spectrophotometer compatible with 96-well plates 1–10 µL single channel pipette 2–20 µL single channel...VWR 76322-150 Pipette tips, 20 µL, VWR 76322-134 Pipettes, 10 mL, VWR 89130-898 1 L polystyrene bottle, ...display a green product. A precipitated product indicates the presence of too much HRP and the need to optimize... and incubated overnight with Anti-Desmin [D7]. Plates were incubated with HRP-linked anti-Human IgG secondary...

...a conformational change of the receptor which modulates the fluorescence intensity of the cpGFP without... vitro, and they further demonstrated that when tested in tobacco protoplasts, the enzymatic biosensor...

...repository  Name Serotype Category Depositor  Notes AAV-GfaABC1D-Cre-4x6T AAV5 Recombinases Stanley...forget to use the link below to keep up with the latest in Addgene's viral vector services and resources...

...: Periodic surveying of cell line collections websites and catalogues Literature searches utilizing Google...correct one!)    Fig. 1: HeLa cells. Image courtesy of Josef Reischig.  Contributing to Cellosaurus...

...of fast bursts, as well as slow up/down voltage states in deep tissue, in vivo. Two of their constructs...recommended for use in western blot, but we are actively testing it in other applications! If you try it out in ...

...with the article metadata, legend and user created notes on a “Pinterest for scientists”? ReFigure extension...profile. To see how to create ReFigures in a few minutes, visit our YouTube Channel. Currently we can only...

... four new plasmid pages that cover some of the hottest CRISPR tools. Base Edit: Base editing plasmids...Plan Your Experiment. #12DaysofCRISPR and blog updates We hope you enjoyed the #12DaysofCRISPR, a celebration...

...pages, performance data, protocols, and even certificates of analysis.” Han’s biggest piece of advice... at eScience Labs, an educational company that creates hands-on science lab kits and digital curriculum...

... to the molecule it detects. This change then generates a reporter signal. Reporter signals may be electrochemical...Biosensor Experiments Experiment  Detection Method Notes Quantification of a process Plate-based assays...

...microbiology with multiple high-profile publications. My classmates consisted of graduate students, post-grads, and...manuscript is like generating a quilt with your lab mates, you add the individual figures and results to make...

...requested from Addgene many times as the technology disseminates throughout the research community. These popular...level system on our website:   A yellow flame indicates that a plasmid has been requested 20+ times   ...