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  1. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    ...has thus benefitted from countless rounds of optimization. Dozens of variants exist with unique cutting...and diversify PAM sequences of Cas12a to further optimize it for therapeutic and laboratory usage. Fast...

  2. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    ...usually 1-3 of the first 3 bps are modified) for optimal stability and efficacy.  Base modifications What...mechanism to turn cutting off or on at a specific time. Recent advances in gRNA modification have overcome...

  3. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    ...“Fix the Knowledge” or “gendered innovations” stimulates excellence in science and technology by integrating... one thing, cells and cell lines degenerate over time—and sex can be hard to determine. To remedy this...

  4. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    ...incoming plasmids are sequenced at least twice, and sometimes more depending on the number of features we need...sequencing reactions. In the more complex cases, we sometimes have to design new primers to sequence the important...

  6. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    ...degradation. Additionally, electron microscopy analysis is time consuming. GFP-tagged Atg8p or LC3: ATG8 is part...4.9 - 9 SEP 488* nm 508 nm ~6.5 - 9 *Optimal sequential excitations wavelengths as determined...

  9. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    ...only one HRP-conjugated RANbody can be used at a time. Samples can be sequentially stained with two HRP...expensive Can only stain with one HRP-RANbody at a time GFP, RFP, H2A2B, Gelsolin Spaghetti Monster: ...

  10. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    ...advantage of this alternative is the possibility of real-time imaging. Robert Singer’s lab at Albert Einstein ...tag after pulse labeling of a determined period of time. Thus, only newly synthesized proteins of interest...

  11. RNA Extraction Without A Kit

    Type
    Blog Post
    ...isolating total RNA, this method requires a lot of time and depending on how many samples you may have, ...isopropanol precipitated. This method reduced the time it took to isolate RNA from 20+ hours to around ...

  12. Plasmids 101: Cre-lox

    Type
    Blog Post
    ...permit gene expression until Cre is present, at which time the gene will be disrupted or deleted. Selection...expressed only in specified cells or at specified times. Combining this with some of the loxP methods described...

  13. Working with Nuclear Receptors

    Type
    Blog Post
    ...be required by one or more NRs for efficient and timely activation or repression of gene expression (see...the research literature and acquiring them in a timely manner. Addgene represents an excellent solution...

  14. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    ...for targeting thousands of long ncRNAs at the same time (Liu et al. 2018). However, removing large lncRNA... the ncRNA (Figure 1D). While this method can be time-consuming, it is relatively effective in reducing...

  15. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    ...companies and start-ups choose to locate there. I made time to go into the city of Guangzhou and see some of...surprising” small RNAs can be and posited that “we underestimate living things.” He further cautioned the distinction...

  20. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ...not only highlighted new tools but also included time for questions and a group discussion on the best... to activation sequences upstream of genes to stimulate expression (Wang et al., 2016). In 2018, the lab...
Showing: 641 - 660 of 925 results