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  2. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    ...successfully labeled protein-coding genes with both intronic and exonic gRNAs. In fact, the method is specific...of the Pitfalls When Using Fluorescent Proteins Control Protein Activity with GFP Resources on Asddgene.org...

  4. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    ...the hands of our finance team, now 3.5 Addgenies strong. The Addgene Board of Directors is mature and...and I believe they know and trust this. We’ve introduced cutting edge practices and benefits and these...

  5. What's New in CRISPR - November 2021

    Type
    Blog Post
    ...for cellular delivery of RNAs By Mike Lacy The retroviral-like protein PEG10 specifically binds its own...small insertions to allow in-frame deletions and introduce epitope tags and RNA transcription start sites...

  6. RNA Extraction Without A Kit

    Type
    Blog Post
    ...degraded. To effectively isolate intact RNA, a fast, strong protein denaturant would be required- something...late 1970’s, Chirgwin and colleagues showed that a strong protein denaturant, guanidinium thiocyanate, did...

  8. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    ...of the two fragments. However, rigidity can be introduced through mutation, allowing diabodies to be used...FluorophoresAntibodies 101: scFVs Antibodies 101: Introduction to Antibodies Additional resources on addgene.org...

  9. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    ...Department at University of Maryland College Park, is a strong supporter of increasing the number of postdoctoral...postdoc-related data collection and analysis. He is also a strong supporter of science Master’s programs. This will...

  10. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    ...their goals.  Check out Joanne's Reddit AMA I am a strong advocate for peer mentoring, but volunteer mentors...contact is Twitter’s @DrQualls. Marquita Qualls of Entropia Consulting works remotely, will travel for speaking...

  11. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    ...institution. Moreover, avoidance of ‘to be’ verbs creates strong, well-directed meaning and delivers strength to...will convince the reviewers that the proposal is strong, well-thought out, and will be successful when ...

  13. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    ...want to knock out a gene, a frameshift mutation introduced anywhere within the early coding region will ...  Susanna Stroik...

  14. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    ...sites on the target protein they tend to have a strong signal and to work in a variety of applications...Postembedding Immunocytochemical Techniques for Light and Electron Microscopy. In Methods in Cell Biology (pp. 283...

  15. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    ... easiest to adapt to genome editing, but the introduction of type V Cpf1-driven systems added another ... endjoining (NHEJ). Cas9-mediated NHEJ usually destroys the PAM site due its proximity to the cleavage...

  17. Lab Automation at Addgene

    Type
    Blog Post
    ...into the robotics spirit include: Start using electronic multichannel and single channel pipettes. These...by one. It seems very simplistic and it isn’t electronic but it is one of the most helpful machines I ...
Showing: 641 - 660 of 915 results