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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 743 results for: UTY;

Showing: 641 - 660 of 743 results

  2. AAV Production in HEK293 Cells

    Type
    Protocol
    ...hydrochloric acid until the solution clears. Check the pH of the solution and use hydrochloric acid or... 1 g/L glucose. 1 mg/mL polyethylenimine (PEI) solution: Dissolve 100 mg of PEI powder into 100 mL of ...to adjust the pH to 7.0. Pro-Tip The pH of this solution will drift pretty rapidly upon addition of acid...over or undershooting the desired pH. Allow the solution to mix for 10 min and then recheck the pH to ensure... ensure that it has not drifted. Filter the solution through a 0.22 μm membrane. Aliquot 500–1000 μL into... Store the tubes at -80 °C. After thawing, the solution can be stored at 4 °C for up to 2 months. After...working stock. 40% POLYETHYLENE GLYCOL (PEG) 8000 solution: Dissolve 400 g of PEG 8000 and 24 g of NaCl ...

  3. Fluorescence Titering Assay

    Type
    Protocol
    ...will be higher titer, consider additional dilutions. Dilution Volume of Lentivirus Stock (μL) Volume of... Mix the dilutions well Gently aspirate media from the cells. Add 1.5 mL of a viral dilution to each well...multiple dilutions. Sample Data Figure 1: 293T cells were transduced with a range of dilutions of pHAGE-TO-dCas9...ensure that you are in compliance with your institution’s biosafety regulations. Workflow Timeline Day...lentiviral aliquot on ice prior to use. Prepare dilutions of the lentivirus into DMEM complete containing... well (each well gets one dilution with one well remaining as the untransduced control). Count the cells...transduction units per mL (TU/mL) using either the dilution factors (method 1) or the volume of virus (method...

  4. Common Injection Routes in Mice

    Type
    Blog Post
    ...compounds, as circulation is slower, and more institutional oversight is required. Restraint: mechanical...

  5. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    ...molecular diagnostics.       Steps of PCR The beauty of PCR is that it can amplify DNA using only a ...saving both time and effort. Scientists can also utilize multiplex qPCR to quantify the concentrations of...

  14. Of Myc and Men

    Type
    Blog Post
    ...kinda. In some cases, antibody providers are distributing monoclonal antibodies derived from the same ...

  15. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ...working with Jane Richardson, provided an initial solution to this problem with the development of Byron’...

  16. Plasmids 101: Degron Tags

    Type
    Blog Post
    ..., AID has gone through an additional round of evolution (AID-2) to reduce some of the leakiness associated...
Showing: 641 - 660 of 743 results