We narrowed to 771 results for: VARS

1. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

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   ... Cancer, Inflammation and Immunity, as well as various combinations thereof (so called “Immuno-Oncology...

2. Improved Plasmid Maps Powered by SnapGene

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   ... - SnapGene’s software seamlessly identifies a variety of common plasmid features such as antibiotic resistance...

3. Multiplex Genome Editing with CRISPR-Cpf1

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   ...the gRNAs and Cas9. Co-transfections can lead to variable expression levels due to differences in copy number...

4. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

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   ...Nanobodies are a small (~15 kDa), single monomeric variable heavy chain antibody domain.  How do these components...

5. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

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   ... fragmented DNA released into the environment. Various factors promote natural transformation in different...

6. 7 Tips to Secure a STEAM Internship This Summer

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   ...doctoral candidate in the Chemical Biology Program at Harvard. Roo wants to simplify access to academic resources...

7. A Quick Guide to a Career in Software Product Management

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   ...will have three or more concurrent projects in varying stages, which can get messy if you’re not on top...

8. 15 Years of Addgene: The Top 15 Plasmids

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   ...expresses Cas9 from Streptococcus pyogenes with an invariant sgRNA scaffold and cloning sites for inserting...

9. Early Career Researcher Toolbox: Social Media for Scientists

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   ...impact your career, 2) given you tips for using various social media platforms, and 3) helped you fit social...

10. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

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    ... Various Addgenies...

11. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

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    ...patient mutations using SDM can help determine if a variant is causal for a given disease. CRISPR has made ...

12. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

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    ...limited packaging capacity. Still confused about the various tRNA expression strategies? No worries, I've summarized...

13. CRISPR 101: Non-Homologous End Joining

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    ...Yan et al., 2020). NHEJ can also be engaged by variants of the canonical Cas9 approach. A pair of CRISPR...

14. Plasmids 101: Yeast Vectors

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    ...non-physiological levels. Scientists have tried varied approaches to combat these issues. One method to...

15. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

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    ...the buck”. Listen to our podcast interview with Harvard Medical School researcher Connie Cepko to learn...

16. Will You Be My Mentor? Finding and Asking for Mentoring Support

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    ...connect with. Listen to our podcast interview with Harvard Medical School researcher Connie Cepko to learn...

17. Addgene: The First Twenty Years

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    ... reagents and protocols so easy!” — Nina Jain, Harvard Medical School. We want to give a special thanks...

18. Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute

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    ...Research Center Work as a Science Writer at the Hutch varies, with no two days being alike. At the time when...

19. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

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    ...transgenes of interest. Furthermore, fluorophore variants (for example green and red fluorescent proteins...

20. Viral Vectors 101: An Introduction to AAV

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    ...PubMed PMID: 26427452. Issa, Shaza S., et al. “Various AAV Serotypes and Their Applications in Gene Therapy...