We narrowed to 771 results for: Vars

1. 15 Years of Addgene: The Top 15 Plasmids

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   ...expresses Cas9 from Streptococcus pyogenes with an invariant sgRNA scaffold and cloning sites for inserting...

2. Why and how to start studying chromatin (with Addgene's help)

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   ...that histone genes frequently have many different variant names, not all of which can be listed here. If ...

3. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

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   ...displayed zero. Unlike Cas9, for which efficiency varies widely across guide RNAs, each guide tested with...

4. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

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   ... Cancer, Inflammation and Immunity, as well as various combinations thereof (so called “Immuno-Oncology...

5. Improved Plasmid Maps Powered by SnapGene

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   ... - SnapGene’s software seamlessly identifies a variety of common plasmid features such as antibiotic resistance...

6. Multiplex Genome Editing with CRISPR-Cpf1

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   ...the gRNAs and Cas9. Co-transfections can lead to variable expression levels due to differences in copy number...

7. Early Career Researcher Toolbox: Social Media for Scientists

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   ...impact your career, 2) given you tips for using various social media platforms, and 3) helped you fit social...

8. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

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   ... Various Addgenies...

9. GPCRs: How Do They Work and How Do We Study Them?

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   ... Various Addgenies...

10. Developing a cold tolerant E. coli using specialized chaperones

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    ...science, our team has continuously engaged with a variety of stakeholders from industrial and commercial ...

11. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

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    ...patient mutations using SDM can help determine if a variant is causal for a given disease. CRISPR has made ...

12. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

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    ...limited packaging capacity. Still confused about the various tRNA expression strategies? No worries, I've summarized...

13. CRISPR 101: Non-Homologous End Joining

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    ...Yan et al., 2020). NHEJ can also be engaged by variants of the canonical Cas9 approach. A pair of CRISPR...

14. Plasmids 101: Yeast Vectors

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    ...non-physiological levels. Scientists have tried varied approaches to combat these issues. One method to...

15. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

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    ...the buck”. Listen to our podcast interview with Harvard Medical School researcher Connie Cepko to learn...

16. Will You Be My Mentor? Finding and Asking for Mentoring Support

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    ...connect with. Listen to our podcast interview with Harvard Medical School researcher Connie Cepko to learn...

17. Addgene: The First Twenty Years

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    ... reagents and protocols so easy!” — Nina Jain, Harvard Medical School. We want to give a special thanks...

18. Supporting Rare Disease Research with the 2020 BeHEARD Awards

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    .... So far, there have been thirty GNB1 missense variants identified from 58 individuals with GNB1 disorder...

19. Viral Vectors 101: An Introduction to AAV

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    ...PubMed PMID: 26427452. Issa, Shaza S., et al. “Various AAV Serotypes and Their Applications in Gene Therapy...

20. The time and cost required to make a plasmid

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    ...designing and performing the experiments. This cost varies hugely based on a number of factors, but we can...