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  1. New Viral Vectors - Fall 2024

    Type
    Blog Post
    ... marker in the various cell types of the basal ganglia. They work via newly-characterized enhancers that...
  2. 10 Ways to Share Your Science!

    Type
    Blog Post
    ... outreach. Here in the North Carolina Research Triangle area, scientists give talks and science cafés ...
  3. Affinity Purification of Recombinant Antibodies with Protein A or Protein G

    Type
    Protocol
    ...Option 2 with a buffer exchange/concentration using an Amicon 30 kDa MWCO buffer exchange column. (Optional... Day 1: Purify antibody Day 2 or later: Buffer exchange Equipment Class II, Type A2 Biological Safety ...above 1.0 mg/mL proceed to Option 1 with a buffer exchange using a Zeba Spin 7 kDa MWCO desalting column....the same recombinant antibody. Section 2: Buffer exchange Choose Option 1 or Option 2 based on the concentration...concentration of the pooled sample above. Option 1: Buffer exchange using a desalting column The 10 mL Zeba Spin Desalting...sterile sodium azide to 1 mM. Option 2: Buffer exchange and concentration with an Amicon column Apply ...least 4 additional times to ensure a full buffer exchange. Discard the flow through into a waste container...
  4. The Strength of Story Telling

    Type
    Blog Post
    ...first author. When I raised this, he was pretty angry and started arguing with me. First I calmed the ...
Showing: 641 - 660 of 827 results