What's New in CRISPR - March 2020
Type
Blog Post
... Jennifer Tsang...
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Same Addgene, New Look - Why We Redesigned Our Homepage & Mascot
TypeBlog Post...homepage recently, you probably noticed some big changes to our site (if you haven’t, go check it out!).... -
Three Key Considerations for Precise Neuronal Targeting Using AAV Technologies
TypeBlog Post...specific neuronal subtypes and determine how these changes affect the phenotype. Viral vectors and genetic... -
A Better Way to Get Customer Support: The Help Center
TypeBlog Post... the entire FAQ collection. You’ll find topics ranging from depositing your lab’s materials and requesting... -
SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture
TypeBlog Post...It is clear that one needs to capture a diverse range of CTCs to cope with the variation in cancer, not... -
The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins
TypeBlog Post...from in the green, red, yellow, cyan, blue, and orange spectra. A comprehensive list of cell markers all... -
Antibody Validation Using the Indirect ELISA Method
TypeProtocol...determine when the desired color change has occurred. The color will change from yellow to blue and generally...experimental conditions. When the desired color change has occurred, gently remove the plate seal. Using...generate a standard curve . If you don’t see any color change or dose response, your antibody concentration may...case, use lower antibody concentrations. The ideal range of the standard curve will vary between targets ...your unknown sample’s absorbance falls above the range of the standard curve you will need to either include... -
SunTag and Fluorescent Imaging
TypeBlog Post...localized, and how its localization and expression may change across various conditions. However, this system... -
Bricking Science: Portraying Scientific Reality Through LEGO
TypeBlog Post...had to purchase the big LEGO classic box, the Big Bang Theory set, and then use our imaginations to mold... -
Tips for Improving Your Next Manuscript
TypeBlog Post...two of our colleagues’ manuscripts and discuss changes to particular sections. The focus groups allowed... -
Antibodies 101: Fc Effector Functions
TypeBlog Post...secondary antibodies to bind. We also know that changing the Fc domain can impact an antibody's binding... -
CRISPR 101: Drug discovery and CRISPR
TypeBlog Post...model can be generated, and drugs screened for a change in pathology. If the disease pathology is complex... -
Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes
TypeBlog Post...expressing ECN is 109 cells/ml, which is within the range of the ~1011 commensal bacteria per gram of stool... -
CUT&RUN: An Improved Method for Studying Protein-DNA Interactions
TypeBlog Post...rely on nuclei extraction. They report that this change offers a significant improvement and simplification... -
Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight
TypeBlog Post...performing a distinct C-bend to escape possible danger (see figure). Larvae also perform spontaneous slow... -
Early Career Researcher Toolbox: Free Tools for Making Scientific Graphics
TypeBlog Post...provide a link to the license, and indicate if changes were made to the images. This requirement means... -
CASTing Off for New Shores in Human Genome Editing
TypeBlog Post..., D. R., Vo, P. L. H., Klompe, S. E., Ronda, C., Wang, H. H., & Sternberg, S. H. (2024). Bacterial genome... -
New Viral Vectors - Fall 2024
TypeBlog Post... marker in the various cell types of the basal ganglia. They work via newly-characterized enhancers that... -
FastCloning: A Newer, Simpler Cloning Technique
TypeBlog Post...construction of specific sequences for base pair overhangs, insert and vector purification, and, most importantly... -
Walking beside Academia and giving it a high five: My transition from graduate work to a position at Addgene
TypeBlog Post...plasmid page here). Now I spend much less time wrangling bacteria and instead help Addgene fulfill its ...