Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 876 results for: cel

Showing: 641 - 660 of 876 results

  1. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type
    Blog Post
    ...fulfilling our mission. “Addgene’s nonprofit mission: Accelerate research and discovery by improving access to...profit motive. We build this trust by keeping excellent records and maintaining confidentiality where ...nonprofit, open science and Addgene in our mission to accelerate research with easier sharing and collaboration...

  2. FastCloning: A Newer, Simpler Cloning Technique

    Type
    Blog Post
    ...properly. Directly transform chemically competent cells with the digested mixture. There is no need for ...purification before transformation. After transforming cells, you should perform colony PCR with vector specific...interested in protein science.     References: 1. Celie, Patrick HN, Annabel HA Parret, and Anastassis Perrakis...

  3. Sensing Neuronal Dopamine

    Type
    Blog Post
    ...the brain has been in vivo microdialysis of extracellular fluid (Darvesh et al., 2011). This technique... across the semipermeable membrane from the extracellular fluid into the perfused liquid. The probe then...detection of dopamine in flies, fish, and mice." Cell 174.2 (2018): 481-496.PubMed PMID: 30007419. PubMed...

  4. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    ...predict that we’d be able to edit RNA in mammalian cells with CRISPR? I certainly didn’t, but now we can ...updates We hope you enjoyed the #12DaysofCRISPR, a celebration of our updated blog resources. Not on social ...Improve HDR Efficiency for CRISPR Editing in Human Cells Day 3: Cpf1: Cpf1: A New Tool for CRISPR Genome ...

  5. RUBY-Red Siliques

    Type
    Blog Post
    ...graduate student studying plant development and cell-cell communication in the Nimchuk Lab in the Biology...untransformed seedlings or callus (undifferentiated plant cell culture), which is great for a wide variety of applications...

  6. Meet the New Addgene Board Members!

    Type
    Blog Post
    ...school at MIT. “I read some of the seminal papers on cell cycle discovery from Lee Hartwell and Paul Nurse...genetic screen to identify the factors that regulate cell division. And that really got me hooked on genetics...high-resolution tracking of more than 1 million cancer cells under drug treatment.” Stegmeier especially finds...

  7. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    ...would continue on with my academic dream of studying cell division. However, after 6 years in the business...Cambridge University where he studied mechanisms of cell division. Recently Seán got the entrepreneurial ...information on ELISA troubleshooting, ELISA kits and cell death assays. You can find Seán on twitter @Macfhearraigh...

  8. Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering

    Type
    Blog Post
    ...and see how different parts of the brain or the cells within them work together or perhaps compete against...two-photon properties, so you could activate single cells in arbitrary patterns in the brain. Addgene: Do ... different – an awake monkey is different from a cell in a dish. I always tell people, 'Be driven by your...

  9. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    ... Typically, Addgene uses electrocompetent Stbl4 cells when performing amplifications, and we use electroporation...DNA. This bias can be counteracted by growing the cells on solid plates, where each transformant forms its...own colony, uninfluenced by the growth of other cells. For libraries where the plasmids are all very similar...

  10. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    ...Voigt CA, Ellington AD. Cell. 2009 Jun 26;137(7):1272-81. doi: 10.1016/j.cell.2009.04.048. PubMed. Find...with sophisticated gene expression controls. Most cell types do not respond to low-intensity light, so ...

  11. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    ...to supplement your cells with biliverdin for maximal brightness. In mammalian cells, one of the improved...Imaging Center at UCSF. A common requirement for live cell imaging experiments is the ability to follow multiple...

  13. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    ...they either trace downstream from the soma (neuron cell body) toward the axon (anterograde tracer) or upstream...particles require receptor-mediated entry into the cell. This means that not all neurons and pathways will...chemogenetic vector directly into the region where the cell bodies of the projection neurons reside.   Impact...

  14. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    ...quantitatively evaluating antibody staining, or counting cells are now routine, open-source software projects that...portable device for studying plankton using flow cells, has become an established alternative to high-priced... and blueprints has the potential not only to accelerate scientific progress, but also to enable science...

  16. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    ...electrocompetent cells in which the phage recombination machinery is induced using L-arabinose. These cells can be...Enzymes Required No No Yes Yes Lab Made Competent Cells Required Yes No No No Comparison of REPLACR-mutagenesis...

  17. Summer SciComm: Preprints

    Type
    Blog Post
    ...from peer-reviewed publishing in a journal.  Accelerating scientific communication Preprints have been...Figure 1: Stand aside, CERN — it's a new age of accelerating science! Photo courtesy of Henry Mühlpfordt,...Lacy was also employed by the American Society for Cell Biology in a position working with preprints in ...

  18. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    ...Professional Career Coaching can be an excellent tactic for scientists making a career shift or who wish...very popular speaker and workshop leader. Lauren Celano of Propel Careers is not a professional coach but...Consulting is part of that group and she is especially excellent in coaching for communication skills. Industry...

  19. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    ...encode proteins. They play important roles in many cellular and regulatory processes, but much of their functionality...screening for functional long noncoding RNAs in human cells by Cas9 targeting of splice sites. Nat Biotechnol...non-coding RNAs with the CRISPR/Cas9 system in human cell lines. Nucleic Acids Research. 2014;43(3):e17. doi...

  20. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    ...Drosophilia Plasmid pRDB_051 (for stable fly cell lines; attL and attR sequences for genome integration...Drosophilia Plasmid pRDB_052 (for stable fly cell lines; Hygromycin resistance gene; for genome integration...rapid assessment of emerging CRISPR technologies. Cell Genomics, 4(3), 100519. https://doi.org/10.1016/...
Showing: 641 - 660 of 876 results