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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 922 results for: tro

Showing: 641 - 660 of 922 results

  1. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    ...operon.   Tips for blue-white screening Use a good control: Transform the backbone plasmid without insert....reproduce to form colonies (used to complement auxotrophic cell lines), or genes which rescue a lethal phenotype...

  2. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    ...other than to sustain those techniques. We feel strongly that the scientific and the agricultural community...aesthetics with this technology and giving consumers control over their interaction with it, we hope to inspire...

  3. Antibodies 101: Fab Fragments

    Type
    Blog Post
    ... means that F(ab’)2 fragments may have overall stronger interaction with their antigens than a Fab or ....2022.100301  Additional resources on the Addgene blog: Introduction to AntibodiesSingle Chain Fragment Variables...

  4. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    ...of the two fragments. However, rigidity can be introduced through mutation, allowing diabodies to be used...FluorophoresAntibodies 101: scFVs Antibodies 101: Introduction to Antibodies Additional resources on addgene.org...

  5. What's New in CRISPR - November 2021

    Type
    Blog Post
    ...for cellular delivery of RNAs By Mike Lacy The retroviral-like protein PEG10 specifically binds its own...small insertions to allow in-frame deletions and introduce epitope tags and RNA transcription start sites...

  6. To Each HIS Own

    Type
    Blog Post
    ...the lab (for a refresher see Antibodies 101: Introduction to Antibodies.) Unfortunately, for some proteins...Protein Tags Antibodies 101: ChIP Antibodies 101: Introduction to Antibodies References Hochuli E, Bannwarth...

  8. RNA Extraction Without A Kit

    Type
    Blog Post
    ...degraded. To effectively isolate intact RNA, a fast, strong protein denaturant would be required- something...late 1970’s, Chirgwin and colleagues showed that a strong protein denaturant, guanidinium thiocyanate, did...

  10. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    ...the hands of our finance team, now 3.5 Addgenies strong. The Addgene Board of Directors is mature and...and I believe they know and trust this. We’ve introduced cutting edge practices and benefits and these...

  11. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    ...successfully labeled protein-coding genes with both intronic and exonic gRNAs. In fact, the method is specific...of the Pitfalls When Using Fluorescent Proteins Control Protein Activity with GFP Resources on Asddgene.org...

  13. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    ...Department at University of Maryland College Park, is a strong supporter of increasing the number of postdoctoral...postdoc-related data collection and analysis. He is also a strong supporter of science Master’s programs. This will...

  14. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    ...their goals.  Check out Joanne's Reddit AMA I am a strong advocate for peer mentoring, but volunteer mentors...contact is Twitter’s @DrQualls. Marquita Qualls of Entropia Consulting works remotely, will travel for speaking...

  15. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    ...institution. Moreover, avoidance of ‘to be’ verbs creates strong, well-directed meaning and delivers strength to...will convince the reviewers that the proposal is strong, well-thought out, and will be successful when ...
Showing: 641 - 660 of 922 results