We narrowed to 968 results for: LAT;
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  TypeBlog Post...publications. The policy was not an overnight revelation, rather it was the result of consultations between...representative or the statistical variation is calculated and placed in the legend. Widely reported difficulties...interfaces where the viewer is more likely to want to manipulate how they view the information. We are used to...
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  Antibodies 101: Introduction to ImmunofluorescenceTypeBlog Post...multiplexing is that you can ask how multiple proteins relate to one another in the same sample. For this strategy...are known to express your target or have been manipulated to overexpress it. For negative controls, use... The biggest difference between IF and ICC/IHC relates to the type of secondary antibody you use, so as...
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  Plasmids 101: Expressing shRNAs for RNA InterferenceTypeBlog Post...the mRNA — the bound miRISC can simply block translation machinery. The composition of the RISC varies...MicroRNA biogenesis, mechanisms of actions, and circulation. Frontiers in Endocrinology, 9. https://doi.org...M. A. (2021). DICER: structure, function, and regulation. Biophysical Reviews, 13(6), 1081–1090. https...
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  10 Steps to a Perfect Science TalkTypeBlog Post...work does relate, make sure to include that material, but don’t force it. You can always relate to scientists... who will be listening. Are they early, mid, or late career? Will many non-scientists be present? Always...
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  Thoughts on the Future of Research 2015 Boston SymposiumTypeBlog Post...speaker Paula Stephan did a fantastic job of articulating many of these problems and some solutions. The...provided as an explanation for why they are paid relatively low wages (Stephan estimates that postdocs are...that PIs no longer save grant money by paying relatively low postdoc salaries as opposed to Staff Scientists...
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  A Control for All SeasonsTypeBlog Post...controls and then moving on to controls for antibody-related applications. After reading, you will have a better...well-studied kinase known to prevent tau hyperphosphorylation. You could run brain samples from both groups... molecular weight shifts indicative of tau phosphorylation. By including the positive control, you will...
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  Transferable Skills Guide: TeamworkTypeBlog Post...the right decision. If I’d spent my senior year isolating pheromones from various C. elegans mutants by ...course, many types of teams. Some teams may be relatively permanent; some teams may only need to exist ...on the other side of the world, communication platforms like Slack make teamwork easier. Trust Ultimately...
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  Which Fluorescence Microscopy Technique is Best for Me?TypeBlog Post...extract light from a single, thin plane within a relatively thicker three dimensional sample (see Figure ...technique should I use for my experiment? When I am contemplating which microscope to use for a new sample I always...optical sectioning (5). One such example is the lattice lightsheet that projects a number of thin cone-...
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  How to Deposit Your Plasmids with AddgeneTypeBlog Post...sequence came from. Entering this information will populate a list of possible Entrez Gene matches to your...easier for other scientists to find information related to your plasmid. It is optional to include the ...correct Entrez Gene does not appear in the auto-populated list, try entering the gene's Official Full Name...
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  Introducing the Addgene Antibodies 101 eBook!TypeBlog Post... first edition of our Antibodies 101 eBook! The latest addition to our eBook collection is a handy resource...start looking for answers. We hope you find the latest addition to our eBook collection useful. And, as...
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  #Deckthelab Contest Winners 2017TypeBlog Post...package with Addgene mugs and some delicious hot chocolate for the lab. Here are some of our 2017 contest...Thank you to everyone who participated and congratulations to our 2017 #Deckthelab winners. We hope everyone...
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  2021 Deck the Lab Winners!TypeBlog Post...festive makeover today✂️❄️🦠thanks to the awesome templates by @CVRHutchinson @CVRinfo. Check them out: https...Mugford (@samtmugford) December 3, 2021 Congratulations to all our winners, and a huge thank you to...
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  New Videos: Addgene Lab TipsTypeBlog Post...automated vial filling, accurate tube-labeling, quick plate pouring, and much more. We didn’t want to keep the...Tip” filter on the blog to keep up to date on the latest episode. Have some suggestions for future tips...
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  Celebrating the 2024 Nobel Prize in ChemistryTypeBlog Post...repository what it is. So we’re thrilled this week to congratulate depositor David Baker, who was awarded the Nobel...developed with their protein design approach. Congratulations to David Baker, Demis Hassabis, and John Jumper...
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  An Interview with Connie Cepko - Gene Therapy, Plasmid Tools and Insights to SuccessTypeBlog Post...proteins as scaffolds for cell-specific gene manipulation.” Cell 154(4) (2013): 928-939. PubMed PMID: ...Tang, Jonathan C.Y., et al. “Cell type-specific manipulation with GFP-dependent Cre recombinase.” Nat. Neurosci...
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  CRISPR Cheat SheetTypeBlog Post...be used to cut the DNA sequence. With a repair template, CRISPR can be used to introduce a functional ...CRISPR nuclease target multiple genes in a cell population. The resulting mutant cells are screened for ...
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  Depositor Week 2020 is Happening Now. Join us!TypeBlog Post... that we can offer the scientific community the latest and greatest plasmid tools! Thank you depositors...globe very quickly. There are over 2,300 COVID-19 related plasmids currently in Addgene’s repository which...
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  Our New Antibody Mascot is....TypeBlog Post...help us prepare for our upcoming antibody launch in late March! *If you submitted the names Abi, Lin...New Antibody Friend We need your help naming our latest mascot, an antibody joining us on our scientific...
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  Celebrating One Million Plasmids Shared!TypeBlog Post... out in time - from assembling the boxes, to inoculating tubes, and to packing and shipping. Every single... This massive scale reagent sharing led us to calculate how much time we have saved researchers. In a ...
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  New Viral Vectors - Spring 2025TypeBlog Post...serotype, and a dTomato under the control of the E6 regulatory element, which targets it to VIP cortical interneurons...forget to use the link below to keep up with the latest in Addgene's viral vector services and resources...