We narrowed to 960 results for: App

1. Antibodies 101: Flow Cytometry Controls

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   Blog Post

   ...ll need a few controls specific to flow as an application. These controls will allow you to distinguish...isotype control. Pro tip! Many companies will have appropriate isotype controls linked on their commercial antibody...

2. Why and how to start studying chromatin (with Addgene's help)

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   ...repair, or replication, or any number of CRISPR applications and thought “OK, but how does this work in the...Chromosome Organization Keystone Symposium. As it happens, there are a lot of scientists who not only remember...

3. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

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   ...reactions by mixing this isolated plasmid DNA with appropriate sequencing primers, and hand deliver them to ...standards are met, we are taking a unique hybrid approach compared to our standard pipeline. For the first...

4. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

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   ... SpCas9. In order to expand the gene-editing applications and targeting scope of ABEs David Liu’s lab ...faster deamination rate so that deamination can happen before Cas homologs let go. The Liu lab developed...

5. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

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   ...researchers (or your own from years ago!) do you really appreciate how few details are provided in most published...important enough to be answered and the methods are appropriate to answer those questions, the article is given...

6. Antibodies 101: Monoclonal Antibodies

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   ... Antibodies: Distinguishing Characteristics, Applications, and Information Resources. ILAR Journal 46:... the Addgene blog Learn more about research applications for antibodies in Intro to Antibodies Read about...

7. GPCRs: How Do They Work and How Do We Study Them?

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   ... the appropriate ligand to activate the G proteins. It is estimated that there are approximately 950 different...

8. GCE4All: Making Genetic Code Expansion Accessible

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   ...function relationships or, in more practical applications, allow a protein to be more effectively used...the creation of many new tools and potential applications. But this focus on development, along with the...

9. Viral Vectors 101: The AAV Retrograde Serotype

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   ...not yet understood, one thing is clear: this application opens a lot of doors in neuroscience. The retrograde...retrograde serotype also has promising therapeutic applications; it can enhance therapeutic delivery across ...

10. Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors

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    ...Juan R. Torruella, U.S. First Circuit Court of Appeals. She served as in-house counsel at St. Elizabeth...responsible for bringing a systematic and disciplined approach to evaluating and improving the effectiveness ...

11. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

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    ...TurboID and miniTurbo podcast segment A fundamental approach to studying membranes, organelles, and other subcellular...directed evolution to create APEX2. They have now applied the same directed evolution technique to the biotin...

12. How Addgene Creates Content

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    ...antibody and content team, we identified what applications should be included and what basic information...we usually make figures in BioRender or use appropriately licensed and cited figures from papers or image...

13. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

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    ...product is then digested and ligated into an appropriate vector. Gibson Assembly, GeneArt Cloning, and...assembly methods are preferred. No such restriction applies to deletions; using REPLACR-mutagenesis, Trehan...

14. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

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    ...over or write to us to get them. Even though we’re happy to provide them, that never works on a large scale..., or words of advice for people who may want to apply this tagging system to their own work? Mendenhall...

15. CRISPR 101: Non-Homologous End Joining

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    ...Here, we’ll discuss NHEJ, and how it impacts what happens to Cas9-mediated DSBs in the genome. Non-homologous...also be engaged by variants of the canonical Cas9 approach. A pair of CRISPR guides that flank regions of...

16. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

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    ...design,” she said. “Before it was a little like tapping in the dark - a lot of trial and error.” The structural...analyzing the data. It should be straightforward to apply the new library for use in various cell types and...

17. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

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    ...to today, Sample6 has changed focus and names, swapped leadership, and grown to 25 members.  What is...competitions. After much learning, the focus of the application was changed, but Sample6 still leverages the ...

18. Plasmids 101: Yeast Vectors

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    ...Instead, the size and A-T content of the DNA (apparently independent of a known specific sequence) dictate...non-physiological levels. Scientists have tried varied approaches to combat these issues. One method to reduce ...

19. Lighting Up Cell Signaling with Photoswitchable Kinases

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    Blog Post

    ...spatiotemporal resolution than pharmacological or genetic approaches, but only a few such methods exist and they only...kinases good for? There are a number of potential applications for photoswitchable kinases.  Below are a few...

20. Addgene: The First Twenty Years

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    ...Addgene is a totally invaluable resource. I really appreciate the work you do to make sharing reagents and ...electronic signatures to dramatically speed up MTA approvals. In 2013 we were thrilled to receive the Massachusetts...