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We narrowed to 966 results for: App

Showing: 661 - 680 of 966 results

  1. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    ...; only two plasmids are required per system. Applications of these systems The large dynamic range of ...suitable for various scientific or engineering applications.   References Refactoring and optimization ...

  2. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    ...wanted to continue doing research, Doench went on to apply to graduate school at MIT. Doench excitedly remembers...Harlow’s group at Harvard Medical School and then applied to work at the Broad Institute: “The model that...

  3. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    ...should die within 3-5 days and resistant colonies appear in about 10-14 days depending on how quickly your...culture to suppress bacterial growth, and is not appropriate for mammalian selection – do not confuse this...

  4. CRISPR Library Amplification

    Type
    Protocol
    ...sufficient quantities of library for experimental applications. Repeated amplifications should be avoided as... of a library of 100,000 plasmids. Colonies may appear small and require extra incubation time in order... as the quantification method. If all Maxipreps appear to contain sufficient DNA for use, pool samples...purifications. Consult the manufacturers handbooks for appropriate volumes and numbers of tandem purifications. ...

  5. Why and how to start studying chromatin (with Addgene's help)

    Type
    Blog Post
    ...repair, or replication, or any number of CRISPR applications and thought “OK, but how does this work in the...Chromosome Organization Keystone Symposium. As it happens, there are a lot of scientists who not only remember...

  6. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    ...methods relied solely on Cas9. Overall, these approaches have two main drawbacks: 1) Most rely on transfection... a simple and effective method with multiple applications (in vitro, in vivo, transfection, viral transduction...

  12. How Addgene Creates Content

    Type
    Blog Post
    ...antibody and content team, we identified what applications should be included and what basic information...we usually make figures in BioRender or use appropriately licensed and cited figures from papers or image...

  13. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    ... Antibodies: Distinguishing Characteristics, Applications, and Information Resources. ILAR Journal 46:... the Addgene blog Learn more about research applications for antibodies in Intro to Antibodies Read about...

  14. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    ...function relationships or, in more practical applications, allow a protein to be more effectively used...the creation of many new tools and potential applications. But this focus on development, along with the...

  15. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    ...ll need a few controls specific to flow as an application. These controls will allow you to distinguish...isotype control. Pro tip! Many companies will have appropriate isotype controls linked on their commercial antibody...

  16. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    ...not yet understood, one thing is clear: this application opens a lot of doors in neuroscience. The retrograde...retrograde serotype also has promising therapeutic applications; it can enhance therapeutic delivery across ...
Showing: 661 - 680 of 966 results