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  2. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    ...familiar with the basics of controls in experimental design, you’ll need a few controls specific to flow as...controls like FMO or isotype controls, which we’ll describe a bit later. Single Colors Single color controls...

  4. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    ...overcomes this limitation and allows for the flexible design and small scale production of antibodies. Problems...Their His tags allow for further purification, if desired, but it's not required for their use. Each RANbody...

  6. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    ...ensure scientists everywhere can access plasmids despite the evolving export/import rules. Our shipping ...most popular website in the world. I know, right? Upgrades to the internal LIMS pages and excellent work ...

  9. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    ...Solution: In Zhou et al, the Lin lab present a generic design for creating photoswitchable kinases and use MEK1...create other photoswitchable kinases? The general design of psMEK1 also worked for creating three other ...

  11. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    ... other sources. Mode of communication Different modes of communication take up different amounts of mental...Maybe I needed to employ a visual aid instead of describing something verbally. Sometimes a group was just...

  13. Addgene: The First Twenty Years

    Type
    Blog Post
    ...business practices, like accepting e-signatures and designing a website users could easily navigate. This meant...Read the Addgene guide: https://www.addgene.org/guides/crispr/” — SirHamhands, responding to a request...

  14. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    ...in one direction. Cas3 is thus not helpful for designer genome edits, but it is effective at wiping out...David, Chen, S., Janice, et. al. Programmed DNA destruction by miniature CRISPR-Cas14 enzymes. Science, 362...

  16. Plasmids 101: Cre-lox

    Type
    Blog Post
    ...using a resistance marker; however, it is often desirable to remove the marker after the initial selection... Combining this with some of the loxP methods described above, a genetic modification can be restricted...

  19. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    ...virus will not only ensure that your infection is designed in the best possible way, but it may also save...the transgene itself. Many researchers prefer to design primers that target the common features of viral...
Showing: 661 - 680 of 985 results