We narrowed to 988 results for: IGH@

...scientists, MTAs are important—they guarantee certain rights and protections for depositor and requestor alike... must be executed. The Golden Stopwatch Award highlights TTOs that accelerate science by processing agreements...

...makes them want to know more. This could be by outright stating the big-picture problem, by asking your...science storytelling. You can introduce your results right at the beginning of your communication, use conflict...storytelling options, and they all can be effective in the right circumstances.   However, in the midst of all these...

...pBR322, pACYC, and pUC were developed to provide higher copy number vectors that could be used in these...updated and added new content to our eBook. Some highlights of the 2nd edition eBook include: new chapters...

...determine genome-wide translational frequency. Although highly useful, this technique requires extensive sample... nucleus to bind cognate untranslated mRNAs. The high specificity and resolution of this system are achieved...SunTag Catch up on Your GFP Background Choose the Brightest Fluorescent Protein Resources on Addgene.org ...

...ask is what to do with them. In some cases, there might be a mutation that disrupts the function of YFG....drivers. It is much easier to mate flies to get the right cell type expression as opposed to making a new ... my next post, I’ll explain the relatively straightforward process of generating your own fly lines. In...

...viral samples. Read on to learn how aqueous samples might be affected by dry ice, and specifically how dry... removing the caps, incubating the tubes in an upright position for 30 seconds, and then recapping the...to prevent any pH change, as we still observed a slight change in pH (from 7.4 to 6.8). It’s possible that...

...as virus? “Like all things in science, getting a high titer Cas9 lentivirus prep takes a bit of time and...lentivirus, we are providing lentiviral preps containing highly requested gRNAs including those targeting EGFP,...library has retained gRNA representation, produce high titer lentivirus from the library, ensure gRNA representation...

...but thought we could learn a little bit more about high throughput DNA prepping by trying out NEB’s new ...generating a wide range of insert sizes and also produced high yields of quality libraries from small amounts of...summarize my experience in my first blog post! I highly recommend doing the same if you ever go to a workshop...

...Director of Biology, Lianna Swanson, suggested that I might be able to attend the 10 Years of iPSCs Symposium...or tertiary, or non-existant). On the very first night of the iPSC symposium, a panel of thought leaders...Resources on the Addgene Blog Learn about Efforts to Fight Rare disease with iPSCs Read About Addgene's Outreach...

...complicated? Well, while the techniques are straightforward, the systems – the cells growing and producing...And on top of that you need to worry about getting high enough expression of your protein of interest. If...by using Addgene’s Blue Flame award to identify highly requested GCE plasmids. But they’d also love scientist...

...variant that efficiently infects neurons, directs high-level transgene expression, and has retrograde functionality...genetic tool delivered by the AAV.   AAVrg requires high titers for efficient retrograde transport. For recombinase-dependent... at the injection site (Fischer, KB. 2019). The higher the titer, the more likely you are to see spurious...

...of mutant target (as low as 20 pg or 0.02%) in a high background of wild type targets. Comparatively, ...M. A Digital PCR-Based Method for Efficient and Highly Specific Screening of Genome Edited Cells. PLoS...PubMed Central PMCID: PMC4735761. 6. Fu, Y. et al. High-frequency off-target mutagenesis induced by CRISPR-Cas...

... synthesized SARS-CoV-2 expression plasmids in a high copy backbone with a T7 promoter driving multiple...GAL1 promoters. These vectors are intended for the high level expression and purification of SARS-CoV-2 ... strengths of intensive screening and the use of high throughput platforms, Addgene and Ginkgo are striking...

...followed by higher stringency PACE, resulted in the evolution of TadA variants with much higher base editing...simultaneously with 54% efficiency, about 7 times higher than ABE7.10. However, the increased editing speed...

...this context, but also specifically study it. They might be researching enhancer elements that control the...research describe the production of plasmids that might be useful for your work. The links below direct ...techniques, the posts below may steer you in the right direction! CAPTURE-ing Chromatin Interactions: Using...

... Directors brings complementary experience and insight to the now 10 member board at Addgene. Let’s meet...Suffolk University Law School. Karen graduated with highest honors from the University of Michigan, Ann Arbor...Ernst & Young, LLP, auditing insurance, finance and high-tech industries. He has earned both an MBA in finance...

...their former interns to fill entry-level positions right out of college. Prior to March 2020, internships... a nationwide database of  STEAM internships for high school, college, and graduate students. In order...interview, inspect your technology, background, and lighting for a smooth performance. Most importantly, make...

...narrow down search results by adding more terms.  Weighting We determined the relative importance of different...given resource, and designed the search engine to weight search results differently according to where a...matches of a name in the depositor field will rank higher than matches of the same name in the publication...

...). As shown on the representative plate to the right, colonies with an insert-containing plasmid have... plates at 4C for few hours after the initial overnight incubation increases pigment precipitation, enhances...colonies. Take care in making your plates: X-gal is light and temperature sensitive and needs to be added ...

...interested in the same things you are on a deep level. Right away you have something in common. Namely, the scientific... field will know about grants and projects that might help your work Tenure can depend on your national...some of my best studies were designed over late night poker games at Gordon Conferences). You don’t want...