We narrowed to 929 results for: INA

1. ReFigure: Save Scientific Figures into Dashboards and Share Your Insights

   Type

   Blog Post

   ...in “negative data” often dominate the early years of a project. Dissemination of the knowledge gained ...

2. Hot Plasmids and Viral Preps - July 2021

   Type

   Blog Post

   ...controllable gene knockouts, and inducible gene inactivation or activation in an organoid model (Sun, et ...activity.  As SLs are also known to trigger seed germination of parasitic plants, this approach was also used...

3. An Addgene Co-op: The Intersection of Fun, Friendship, and Work

   Type

   Blog Post

   ...at one of my first “All Addgene” meetings, when Melina, our CSO and co-founder, approached me and asked...coworkers were only a Slack message away.  When it finally came time for me to present, I thought I would ...

4. CRISPR 101: Drug discovery and CRISPR

   Type

   Blog Post

   ... or have a simple pathology. In these cases, preliminary identification of potential targets by CRISPR...mutations and then test both individual and combinatorial drugs on for effectiveness prior to patient ...

5. Hot Plasmids - October 2022

   Type

   Blog Post

   ... reliable antibodies. It is derived from the C-terminal region of the human proto-oncogene c-Myc. Since... catalog pulled up over 13,000 plasmids! Our recombinant anti-c-Myc [9E10] (isotype IgG2a) antibody is...

6. FastCloning: A Newer, Simpler Cloning Technique

   Type

   Blog Post

   ... isolation, insertion and amplification of a recombinant DNA without sequence alteration. Molecular cloning...Annabel HA Parret, and Anastassis Perrakis. "Recombinant cloning strategies for protein expression." Current...

7. Management for Scientists: Managing vs. Leading

   Type

   Blog Post

   ...this quality.   I am often asked to give career seminars on Leadership Skills. After attempting to put ...efficiently and with high quality. Their work involves coordination and can require technical mastery. Scientists...

8. History of CRISPR Cas - A tale of survival and evolution

   Type

   Blog Post

   ... biotech field, let's look at how prokaryotes originally utilized CRISPR/Cas as a powerful and adaptive...expression of 1 of 5 anti-CRISPR genes were found to inactivate the Type I-F CRISPR/Cas system of Pseudomonas...

9. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

   Type

   Blog Post

   ...beamsplitter, and a 495/30 nm emission filter. This combination results in a 2.6-fold increase in signal relative...fluorescent protein: identification of the molecular determinants of color and applications in fluorescence imaging...

10. Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

    Type

    Blog Post

    ...specific genomic loci. He made use of catalytically inactive dCas9 by fusing it to an engineered peroxidase...Using these lentiviral plasmids, catalytically inactive Cas9 (dSpyCas9) fused to APEX2 is expressed from...

11. CRISPR Challenges: Standardization and Homology Directed Repair

    Type

    Blog Post

    ...notes, since the conference, I think I may have finally grasped just how complicated DNA repair pathways...genome editing using oligonucleotide donors". Kan, Yinan, Brian Ruis, Taylor Takasugi, and Eric A. Hendrickson...

12. Intro to Our Summer SciComm Series!

    Type

    Blog Post

    ...roughly) the same field, often through manuscripts, seminars, or posters. It is important to note that it is...does not explicitly exclude people outside of the original recipients. Instead, it targets a specific audience...

13. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type

    Blog Post

    ...will help get your experiments back on track.  One final note — this post focuses on the species-on-species... Fragment (scFvs) Resources on Addgene.org Recombinant antibodies for IHC at Addgene  ...

14. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type

    Blog Post

    ...– I want to understand my obsession with the combination of chocolate and peanut butter), but it’s an ...Using a programmable microcontroller, NeuroStack coordinates optical stimulation of its associated fibers ...

15. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type

    Blog Post

    ... THE ORIGINAL NgAgo ARTICLE DISCUSSED IN THIS POST HAS BEEN RETRACTED AND FOLLOW UP STUDIES HAVE FAILED...prevent the invading genome from “recovering” its original sequence. If you’re looking to disrupt gene function...

16. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

    Type

    Blog Post

    ...generic term for any extrachromsomal hereditary determinant”. His proposal, however, was basically ignored... in areas such as bacterial conjugation and recombination, replication and topology, and cloning and gene...

17. Don’t Wait To Launch Your Health Science Startup

    Type

    Blog Post

    ...critical three letters after your name (P-H-D).  Finally, having spent your entire career in science, you... you see today is far different than what was originally planned, due in large part to the feedback we...

18. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type

    Blog Post

    ...Bob Goldstein’s lab at the University of North Carolina has made CRISPR in C. elegans even easier - now...elegans genome using Cas9-triggered homologous recombination. Dickinson DJ, Ward JD, Reiner DJ, Goldstein...

19. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type

    Blog Post

    ...transcriptional control of multiple loci is deftly coordinated by the eukaryotic cell for the execution of many...University of Western Ontario studying plant-based recombinant pharmaceuticals. Follow him on Twitter @AdamChinFatt...

20. Extracurricular Activities for a Strong Science Career Path

    Type

    Blog Post

    ...graduate and postdoc training focuses on using multidisciplinary, collaborative, and translational approaches...“Never Leave an Empty Seat” at conferences and seminars, as this is a valuable time to network Attend...