We narrowed to 1,082 results for: IND

...widely used class of protein-binding reagents. In the lab, their binding specificity allows scientists.... The ends of the branches contain the antigen binding sites and are highly variable, allowing exquisite...The immortalized cells, called hybridomas, are individually isolated and expanded. The clonal cells are ...

...calcium binding protein) fused to two fluorescent proteins. When the responsive protein binds to calcium...Venus fluorescent protein, the Troponin C Ca++ binding domain, and NanoLuc® Luciferase. CalFlux VNT responds...2017) Genetically encoded bioluminescent voltage indicator for multi-purpose use in wide range of bioimaging...Plasmids Browse the Fluorescent Protein Collection Find Fluorescent Protein Biosensors ...

...in a variety of cell types, but you should try to find the best AAV for your particular experiment! Traveling...previous literature using AAVs for DREADD delivery indicates ~7-21 days are required for the onset of gene ...neural tracing in very specific cell populations. Find Ready-to-Use Retrograde AAV Preps at Addgene 2) ...scAAV) vectors promote efficient transduction independently of DNA synthesis." Gene therapy 8.16 (2001):...Vector Elements Additional Resources on Addgene.org Find AAV Vectors for Your Research Get Ready-to-Use AAV...

...expression of Red include the IPTG-inducible lac promoter, the arabinose-inducible pBAD promoter and the endogenous...direct modification of DNA within E. coli and is independent of restriction sites. The lambda red system is...with a higher efficiency than the other and you’ll find out empirically which is the lagging strand. ssDNA...Resources on Addgene.org Browse our Microbiology Pages  Find Lambda Red Plasmids and Strains Check Out Other ...

... used during the purification procedure. Good indicators of DNA purity are the absorbance measurements...nm should fall ~1.8 - 2 and give scientists an indication of purity versus protein contaminants. The 260...nm ratios should fall ~2 - 2.2, and provide an indication of purity versus chaotropic agents as guanidine...Addgene.org Read the molecular biology reference Find plasmids for your research Brush up on molecular...

... down What happens if you do scroll down? You’ll find Blugene, as well as information about Addgene, our...our three most recent blog posts. Finally, you’ll find everyone’s favorite section: Tools of the Day. Our... obvious ones to discover as you browse. You may find one of your most frequently used buttons stands ...faster to skim through. Users with disabilities will find Addgene’s website easier to navigate, thanks to ...

...Website Visit our Lentiviral Guide Pages Find Lentiviral Plasmids Find CRISPR Pooled Libraries ...We’ve dug into the data from our repository to find our most requested plasmid in 2015. In line with ...lentiCRISPR v2, you can take a look at the protocol kindly provided by the Zhang lab. Even though lentiCRISPR...

...) suggestions for defining the problem and for finding solutions. Even better than just reading this article...Berkeley and Harvard. Students also partner with independent career organizations to get the grants and programs...There are many academic administrators who are finding ways to provide new types of career preparedness...years. However, some organizations are woefully behind and some just don't have the resources to do enough...

...could be managing a team, planning meetings, and finding the best way to organize your time. At a conference...social media, you could be sharing your research or finding opportunities to bring science to the community...you love, you have to create opportunities. If you find yourself asking, "Why doesn't this product/organization...transferable skills guide Resources on Addgene.org Find job listings at Addgene Get more career insight ...

...lab that has multiple collaborations, even with Industry labs Work on a big project that relies on a division...group to get real feedback Write whenever you can, find supportive editors so your first drafts are always...inviting others (be on a speaker committee) Follow industry publications like FierceBiotech to “learn the .... For More Reading: From Academic Solos to Industrial Symphonies by Gwen Acton, Alicia Gómez-Yafal ...

...In that time, Addgene has shipped over 350,000 individual plasmids to 5,000 different research institutions...institutions. This has given us a unique window into technology trends in the life sciences. In this post, we'll...two most popular plasmids of the past 10 years. Induced Pluripotent Stem (iPS) Cells - 2008 was marked ... GCaMP6 series of genetically encoded calcium indicators were the hot technology of the year in our collection...

...The results are light-inducible transcriptional effectors (LITEs) designed to bind specific genes and turn...Zhang’s lab at MIT, the use of customizable DNA-binding domains based on transcription-activator-like effectors...methyltetrahydrofolate, which are universally abundant. Second, induction of transcription occurs within minutes of light...

...mechanism. The Cas13 effector binds the guide RNA (shown as mature crRNA) and binds the RNA target before initiating...often recruits the main effector complex after binding to a target. Class 1 is the most abundant of the... functions as both a helicase and a nuclease, unwinding and cutting up DNA after recruitment by Cascade... large-scale clustering and discovery algorithms find new candidates for CRISPR proteins. A type VII is...

...Fluorescent Protein Guide Pages Find Plasmids for Subcellular Localization Find Fluorescent Protein Empty Vectors...jump from one antigen presenting cell to another. Indeed, we now know that T cells are able to crawl more...proteins (proteins that fluoresce after a light-induced chemical reaction) or photoconvertible Keade proteins...proteins (protein that undergoes light-induced irreversible photoconversion from green fluorescence to...

.... Resources at Addgene Find optogenetics plasmids! Find AAV resources! Find fluorescent protein resources...the practicalities and considerations you may not find in the materials and methods sections of optogenetics...Cre-dependent optogenetic transgenic mice for light-induced activation and silencing. Nature neuroscience. ...

...determine if there have been any early successes, and to find out what delivery techniques researchers are using...posts throughout the month including a first of its kind for the Addgene blog - Addgene Software Engineer...programming noob (like myself) can understand. You’ll also find actionable advice on how to give a great science... (hopefully non-vacationing) scientists. You can find all of our August posts in chronological order here...

...sabatini-wood-cancer-pathway-orfs/ Finding signaling pathways involved in drug resistance Wood wanted to find out which particular...Additional Resources on Addgene.org Find Other Kits for Your Research Find Pooled Libraries for Your Research...

...Violet-excited GFP (vex) Rosa26-Cas9 I-CHIME Inducible knockout of a single gene pXPR_068 Violet-...immunotherapy targets but are embryonic lethal, hindering studies post embryogenesis. Using C-CHIME and ...preparations than the first generation CHIME (pXPR_053). Find X-CHIME plasmids here!   Marty LaFleur is a Postdoctoral... A. H. (2024). X-CHIME enables combinatorial, inducible, lineage-specific and sequential knockout of genes...

...posts Resources on Addgene.org Find plasmids for your research Find cancer research plasmids and resources...amplifications and fusions, missense mutations, and indels (Ng et al., 2018). From this data, they generated...The researchers used the collection to screen individual perturbations in vitro using Ba/F3 and MCF10A...

...plant, Drosophila and mammalian cells, and it may find wide use in biological research. Photoconvertible...color was observed after excitation at 488 nm, indicating that spontaneous conversion did not occur. Tobacco...protein. Nat Methods. 2009 Feb 6(2):131-3.  PubMed. Find plasmids from this publication at Addgene. Subach...31;8(9):771-7. doi: 10.1038/nmeth.1664. PubMed. Find plasmids from this publication at Addgene.   Addgene...