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We narrowed to 1,076 results for: IND

Showing: 661 - 680 of 1076 results

  1. Important Considerations When Using AAVs

    Type
    Blog Post
    ...in a variety of cell types, but you should try to find the best AAV for your particular experiment! Traveling...previous literature using AAVs for DREADD delivery indicates ~7-21 days are required for the onset of gene ...neural tracing in very specific cell populations. Find Ready-to-Use Retrograde AAV Preps at Addgene 2) ...scAAV) vectors promote efficient transduction independently of DNA synthesis." Gene therapy 8.16 (2001):...Vector Elements Additional Resources on Addgene.org Find AAV Vectors for Your Research Get Ready-to-Use AAV...

  2. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

    Type
    Blog Post
    ...expression of Red include the IPTG-inducible lac promoter, the arabinose-inducible pBAD promoter and the endogenous...direct modification of DNA within E. coli and is independent of restriction sites. The lambda red system is...with a higher efficiency than the other and you’ll find out empirically which is the lagging strand. ssDNA...Resources on Addgene.org Browse our Microbiology Pages  Find Lambda Red Plasmids and Strains Check Out Other ...

  3. Addgene's Website Update

    Type
    Blog Post
    ... down What happens if you do scroll down? You’ll find Blugene, as well as information about Addgene, our...our three most recent blog posts. Finally, you’ll find everyone’s favorite section: Tools of the Day. Our... obvious ones to discover as you browse. You may find one of your most frequently used buttons stands ...faster to skim through. Users with disabilities will find Addgene’s website easier to navigate, thanks to ...

  4. Plasmid Preps: Different Purity, Different Quantities, Different Uses

    Type
    Blog Post
    ... used during the purification procedure. Good indicators of DNA purity are the absorbance measurements...nm should fall ~1.8 - 2 and give scientists an indication of purity versus protein contaminants. The 260...nm ratios should fall ~2 - 2.2, and provide an indication of purity versus chaotropic agents as guanidine...Addgene.org Read the molecular biology reference Find plasmids for your research Brush up on molecular...

  5. Your Top Requested Plasmid in 2015!

    Type
    Blog Post
    ...Website Visit our Lentiviral Guide Pages Find Lentiviral Plasmids Find CRISPR Pooled Libraries ...We’ve dug into the data from our repository to find our most requested plasmid in 2015. In line with ...lentiCRISPR v2, you can take a look at the protocol kindly provided by the Zhang lab. Even though lentiCRISPR...

  6. A Conference By Postdocs For Postdocs: Future of Research

    Type
    Blog Post
    ...) suggestions for defining the problem and for finding solutions. Even better than just reading this article...Berkeley and Harvard. Students also partner with independent career organizations to get the grants and programs...There are many academic administrators who are finding ways to provide new types of career preparedness...years. However, some organizations are woefully behind and some just don't have the resources to do enough...

  7. Download Addgene’s Science Career Guide, Plus 8 Career Tips from Addgenies

    Type
    Blog Post
    ...could be managing a team, planning meetings, and finding the best way to organize your time. At a conference...social media, you could be sharing your research or finding opportunities to bring science to the community...you love, you have to create opportunities. If you find yourself asking, "Why doesn't this product/organization...transferable skills guide Resources on Addgene.org Find job listings at Addgene Get more career insight ...

  8. Developing Transferable Skills During Science Training

    Type
    Blog Post
    ...lab that has multiple collaborations, even with Industry labs Work on a big project that relies on a division...group to get real feedback Write whenever you can, find supportive editors so your first drafts are always...inviting others (be on a speaker committee) Follow industry publications like FierceBiotech to “learn the .... For More Reading: From Academic Solos to Industrial Symphonies by Gwen Acton, Alicia Gómez-Yafal ...

  9. The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years

    Type
    Blog Post
    ...In that time, Addgene has shipped over 350,000 individual plasmids to 5,000 different research institutions...institutions. This has given us a unique window into technology trends in the life sciences. In this post, we'll...two most popular plasmids of the past 10 years. Induced Pluripotent Stem (iPS) Cells - 2008 was marked ... GCaMP6 series of genetically encoded calcium indicators were the hot technology of the year in our collection...

  10. Let There Be LITE Plasmids

    Type
    Blog Post
    ...The results are light-inducible transcriptional effectors (LITEs) designed to bind specific genes and turn...Zhang’s lab at MIT, the use of customizable DNA-binding domains based on transcription-activator-like effectors...methyltetrahydrofolate, which are universally abundant. Second, induction of transcription occurs within minutes of light...

  11. Typing CRISPR Systems

    Type
    Blog Post
    ...mechanism. The Cas13 effector binds the guide RNA (shown as mature crRNA) and binds the RNA target before initiating...often recruits the main effector complex after binding to a target. Class 1 is the most abundant of the... functions as both a helicase and a nuclease, unwinding and cutting up DNA after recruitment by Cascade... large-scale clustering and discovery algorithms find new candidates for CRISPR proteins. A type VII is...

  12. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type
    Blog Post
    ...Fluorescent Protein Guide Pages Find Plasmids for Subcellular Localization Find Fluorescent Protein Empty Vectors...jump from one antigen presenting cell to another. Indeed, we now know that T cells are able to crawl more...proteins (proteins that fluoresce after a light-induced chemical reaction) or photoconvertible Keade proteins...proteins (protein that undergoes light-induced irreversible photoconversion from green fluorescence to...

  13. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    .... Resources at Addgene Find optogenetics plasmids! Find AAV resources! Find fluorescent protein resources...the practicalities and considerations you may not find in the materials and methods sections of optogenetics...Cre-dependent optogenetic transgenic mice for light-induced activation and silencing. Nature neuroscience. ...

  14. Editor's Choice, August 2016

    Type
    Blog Post
    ...determine if there have been any early successes, and to find out what delivery techniques researchers are using...posts throughout the month including a first of its kind for the Addgene blog - Addgene Software Engineer...programming noob (like myself) can understand. You’ll also find actionable advice on how to give a great science... (hopefully non-vacationing) scientists. You can find all of our August posts in chronological order here...

  15. Modulate the Activity of 17 Signaling Pathways with One Kit!

    Type
    Blog Post
    ...sabatini-wood-cancer-pathway-orfs/ Finding signaling pathways involved in drug resistance Wood wanted to find out which particular...Additional Resources on Addgene.org Find Other Kits for Your Research Find Pooled Libraries for Your Research...

  16. X-CHIME: Context Dependent Germline Knockout in Immune Cells

    Type
    Blog Post
    ...Violet-excited GFP (vex) Rosa26-Cas9 I-CHIME Inducible knockout of a single gene pXPR_068 Violet-...immunotherapy targets but are embryonic lethal, hindering studies post embryogenesis. Using C-CHIME and ...preparations than the first generation CHIME (pXPR_053). Find X-CHIME plasmids here!   Marty LaFleur is a Postdoctoral... A. H. (2024). X-CHIME enables combinatorial, inducible, lineage-specific and sequential knockout of genes...

  17. A new platform for fast functional characterization of potentially cancerous mutations

    Type
    Blog Post
    ...posts Resources on Addgene.org Find plasmids for your research Find cancer research plasmids and resources...amplifications and fusions, missense mutations, and indels (Ng et al., 2018). From this data, they generated...The researchers used the collection to screen individual perturbations in vitro using Ba/F3 and MCF10A...

  18. Searchable and Sortable gRNAs for Your Next CRISPR Experiment

    Type
    Blog Post
    ...testing entirely new gRNA designs. You can now easily find many validated gRNAs in our newly curated Validated...search for ‘sapiens activate’ to filter the table and find exactly what you're looking for. Search using the...alternate names, particularly for gene names. If you find a sequence that will work for your experiment, it...Browse gRNA Design Tools Read Our CRISPR Guide Pages Find CRISPR Plasmids  ...

  19. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    ...plant, Drosophila and mammalian cells, and it may find wide use in biological research. Photoconvertible...color was observed after excitation at 488 nm, indicating that spontaneous conversion did not occur. Tobacco...protein. Nat Methods. 2009 Feb 6(2):131-3.  PubMed. Find plasmids from this publication at Addgene. Subach...31;8(9):771-7. doi: 10.1038/nmeth.1664. PubMed. Find plasmids from this publication at Addgene.   Addgene...

  20. IDP and your PI: A Roadmap for Career Planning and Personal Development

    Type
    Blog Post
    ...out a career path? Many trainees are developing Individual Development Plans (IDP's) through Science Careers...easy to miss out on beneficial opportunities or to find oneself unsure of the next steps. Harvard PI Angela... look the same in every lab, but the principles behind this technique are beneficial for researchers at...   References 1. Yearly Planning Meetings: Individualized Development Plans Aren't Just More Paperwork...
Showing: 661 - 680 of 1076 results