We narrowed to 912 results for: IRE;

...reverse transcriptase (RT) template including the desired edit. Figure 1: Prime Editing schematic....guide Find CRISPR plasmids by function Browse our entire plasmid collection ...

...functional loss (Ho et al. 2014). To overcome this, paired gRNAs can be used to target specific sizes of a...visualizing ncRNA localization and function, but directly incorporating very long ncRNAs is impractical....

...antibody affinity: stronger binding antibodies will require harsher stripping to remove, so start with the ...with caution. Unlike PVDF, they can be returned directly to buffer without a re-wetting step. Important...

... inhibitor response 1 (TIR1) expressed under a desired promoter with a specific spatiotemporal expression...motif (PAM). They're used to determine the PAM requirements of Cas enzymes. CRISPR-ARE is a new system from...

...Just like with any sport, hours of practice are required and no one lab or professor becomes an overnight...they are looking for in grant submissions and will direct you on how to submit your work either through seminars...

...capabilities. Specifically this includes primer directed isothermal RNA amplification, new toehold switch...samples; we showed that we can detect the virus directly from infected monkey blood plasma without purification...

...suggestions and take your project in the wrong direction. The best way to avoid miscommunication is to ...proactive person that all employers are eager to hire. An excellent resource to learn more about assertiveness...

...their academic science careers in order to head to Ireland and take a chance as entrepreneurs. To make things...in fact, our color changing flower project was inspired by a Facebook comment.  In our day job, we worked...

...used to push your research in new and exciting directions. Drosophila are very easy to manipulate genetically...fly-based experimental systems. Some resources will direct you to the latest information about gene function...

...without limitations and well-executed experiments require some consideration of FP properties. It is important...detected in microscopy cannot be interpreted as direct molecular interaction without further investigation...

.... A special class of FPs, the FP timers, add an entire new dimension to monitoring localization; using...emission and absorption spectra). Subach et al’s directed mutagenesis also produced the three separate timers...

...poster: those that want you to walk them through the entire poster, or those that want to look through themselves...handle on your nametag for the tweep up (and for the entire conference, in general). After the conference Review...

...Optimized dicot prime editing enables heritable desired edits in tomato and Arabidopsis. Nature Plants,..., M. D., Wu, J. C., & Garcia-Marcos, M. (2024). Direct interrogation of context-dependent GPCR activity...

...in the depositing lab, although you may have an entirely different use in mind! This section of our plasmid...in the E. coli lab strain DH5a, but others may require strains such as ccdB survival (for Gateway plasmids...

..., the drive could eventually spread through the entire wild population with unpredictable ecological effects...ecological, and reproductive confinement typically require very little effort to implement (depending on the...

...tools work in any plant? Patron: The MoClo kit is entirely for dicots, not grasses and wheat, but we do work... Patron: I started my Twitter account recently inspired by an academic in literature who gave a talk to...

...emission is green or red light. For example, T-Sapphire, LSSmOrange, and LSSmKate. Fluorescent Sensors...many FPs (particularly those derived from GFP) requires oxygen. Therefore, these FPs cannot be used in...

...think of as an operating system for engineering an entire cell. Typically when people use plasmids, they ...much easier. So you could imagine engineering the entirety of a cell. That's a big long-term technical challenge...

... that if an essential gene plays a role in the desired screening phenotype, it may be missed because it's...transcriptional machinery to dCas9 and its RNA directed binding site. These methods include the fusion...

...modeling isn’t up your alley, feel free to skip directly to the results. To start modeling our data, we...chemistry and chemical biology.       David R. Liu is Director of the Merkin Institute and Vice-Chair of the ...