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We narrowed to 1,039 results for: LAS

Showing: 661 - 680 of 1039 results
  1. Celebrate National Book Month with Addgene

    Type
    Blog Post
    ...poetry, and I’ve been reading a poem a day for the last 8 years. Since then, we’ve read books across nearly..., true crime, and so on. We’ve read well-known classics, like Austen’s Pride and Prejudice, and some more...
  2. DAP arrays for multiplex gene editing

    Type
    Blog Post
    ...tRNA-gRNA arrays, which is scalable and efficient in plasmid and viral vectors. In applications when repetitive...manual and online design examples of using Addgene plasmids hCtRNA_FT (#186715) and hCtRNA_VT (#186716).   ...
  3. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    ... between two halves of a bacterially derived periplasmic glutamate binding protein known as GltI. When...virus aliquots being deposited at Addgene. Other plasmids and pAAVs can be ordered directly from Addgene...
  4. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    ...validated editing reagents can be found on our plasmid pages. We encourage any labs performing genome ...CRISPR Background with Our CRISPR Guide Find CRISPR Plasmids for Your Research Check out Our List of gRNA Design...
  5. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    ...spectra of your protein with the filter sets or lasers on your microscope. Ideally, you would like to ...Rhodamine / Cy5 (e.g. Semrock's) and the similar 4-laser combination on a confocal (405 / 488 / 561 / 640...
  6. Antibodies 101: Flow Cytometry Controls

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    Blog Post
    ...be from the same host species, have the same Ig subclass, and be in the same fluorophore (e.g., if you ...staining for anti-mouse Ly6C in APC with the IgG2c subclass, you will use the APC IgG2c isotype control). ...
  7. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    ...open doors to a web of opportunities that the classroom cannot offer. Furthermore, companies across most... letter that is customized for each internship. Lastly, have your resume and cover letter revised by your...
  8. Giving gRNAs a Facelift - Synthetic and Beyond

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    Blog Post
    ...RNA molecules are vulnerable to attack by both cytoplasmic and nuclear nucleases, as they will be recognized...ready-to-use viral preps of select CRISPR lentiviral plasmids Additional resources on the Addgene Blog  How...
  9. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    ... can clone the genes from the hybridoma into a plasmid to create a recombinant antibody.     Figure...Resource on Addgene.org Check out Addgene’s antibody plasmid collection Read the latest on Addgene's new antibody...
  10. Antibodies 101: Fab Fragments

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    Blog Post
    ... the lab. What is a Fab Fragment? Consider the classic IgG antibody (Figure 1): it is composed of four... (more on when that might be later). Find scFv plasmids at Addgene Depending on how an antibody is broken...
  11. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    ...contain two numerical designations, separated by a slash, that indicate the ITR and serotype, respectively...of both polarities are rescued from the vector plasmid and individually packaged into the AAV capsids....
  12. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    ...for an undergrad taking their first upper-level class. Even so, it’s quite easy (and common!) to be blind... can understand and process your information. A class of three year olds is unlikely to be able to follow...
  13. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    ... create fusions with a gene of interest. These plasmids will be most useful for constructing intermolecular...PMID: 27475975. 6. Gayrard, Charlène, and Borghi, Nicolas. "FRET-based molecular tension microscopy." Methods...
Showing: 661 - 680 of 1039 results