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We narrowed to 1,035 results for: LAS

Showing: 661 - 680 of 1035 results

  1. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    ...for an undergrad taking their first upper-level class. Even so, it’s quite easy (and common!) to be blind... can understand and process your information. A class of three year olds is unlikely to be able to follow...

  3. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    ...contain two numerical designations, separated by a slash, that indicate the ITR and serotype, respectively...of both polarities are rescued from the vector plasmid and individually packaged into the AAV capsids....

  4. Antibodies 101: Fab Fragments

    Type
    Blog Post
    ... the lab. What is a Fab Fragment? Consider the classic IgG antibody (Figure 1): it is composed of four... (more on when that might be later). Find scFv plasmids at Addgene Depending on how an antibody is broken...

  5. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    ...RNA molecules are vulnerable to attack by both cytoplasmic and nuclear nucleases, as they will be recognized...ready-to-use viral preps of select CRISPR lentiviral plasmids Additional resources on the Addgene Blog  How...

  9. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    ... create fusions with a gene of interest. These plasmids will be most useful for constructing intermolecular...PMID: 27475975. 6. Gayrard, Charlène, and Borghi, Nicolas. "FRET-based molecular tension microscopy." Methods...

  11. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    ...until now. Alice Ting’s lab has developed a new, plasmid-based biosensor to visualise changes in histone...Additional Resources at Addgene Find the Ting Lab plasmids at Addgene Browse More Fluorescent Protein Biosensors...

  12. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    ...methods for the determination of amplification of Plasmodium falciparum multidrug resistance-1 gene (pfmdr1... RJ, Paterson ES, Smith ML. The copy-number of plasmids and other genetic elements can be determined by...

  14. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ... The most obvious workaround is to use a multi-plasmid system, where you have one AAV vector dedicated...interrogate immune cells to improve immunotherapy in glioblastoma, and generate programmable screening platforms...

  15. Common Injection Routes in Mice

    Type
    Blog Post
    ...Engineering Guide CRISPR Mammalian Expression plasmids Cre-Lox plasmids References Prathipati, P., Rodriguez-Aguayo...

  17. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    ...community. I even spray painted a small erlenmeyer flask with gold glitter spray paint to make a trophy for...Career Posts Resources on the Addgene.org Find Plasmids for Your Research Learn About A Variety of Different...

  19. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    ...been developed into retroviral vectors and 2.) a subclass of retroviruses called the lentiviruses (like ...Addgene's Lentiviral Guide Most popular lentiviral plasmids γ-Retrovirus Guide References Cullen, B. R. (...
Showing: 661 - 680 of 1035 results