...targeting the genomic site of interest. The donor template is supplied separately. To use this system, you... 37 °C.     The new pEcCas/pEcgRNA system We speculated that the gRNA on pCas that is specific to the... in the lack of recovered transformants. Our speculation motivated us to update the pCas/pTargetF system...

...unlikely candidate. It was discovered as an antigen upregulated on activated lymphocytes and is commonly used...driving the PHP.B phenotype. Exome sequencing of related hybrid mice narrowed the search to a ~4.5 Mbp region...adeno-associated virus-PHP. B capsid fails to upregulate transduction efficiency in the marmoset brain...

...different bacterial hosts (Doran et al. 1998). Regulatory elements within the ori also control copy number...which is the most commonly used bacteria in lab manipulations. Table 1 has a detailed list of the bacterial...leguminosarum, R. meliloti, Rhodobacter sphaeroides, R. capsulatus, Rhodopseudomonas spheroides, Salmonella spp....

...ummmm, what is it? Reddit is a volunteer-organized platform where users can participate in forum discussions...to see the questions first makes Reddit a great platform for those who aren’t so great at coming up with...whole point is to meet actual people to build relationships. I also like Twitter because I can get a little...

...modifiers available. This system utilizes an RNA template containing the desired edit adjoined to the sgRNA...affect gene expression? dCas9 can be used as a platform to deliver various cargo to a specific DNA locus...single transcriptional activation domain - VP64.  Later generations of activators include the SunTag system...

...several genes previously not associated with ricin regulation, including almost all genes involved in the production... screen did fail to identify some known ricin-regulators, but most of these genes are also essential for...increased and decreased p-values of known ricin regulation genes compared to results generated using non-targeting...

...path, you’ve probably got a gene you’d like to manipulate in mind. Now it’s time to consider where you ...mice once they have been generated (more on that later). Find Validated gRNAs to Target Mouse Genes To ...biology and genetics. She loves to communicate the latest and greatest in scientific content.      References...

...enough to promote cell growth. Overgrown cultures stimulate quiescence, the resting phase of the cell cycle...that the results obtained in these lines can be translated to other lines. Differences in the physiology...therefore titers obtained in one cell type may not translate well to other cell types. To overcome this issue...

...publications. The policy was not an overnight revelation, rather it was the result of consultations between...representative or the statistical variation is calculated and placed in the legend. Widely reported difficulties...interfaces where the viewer is more likely to want to manipulate how they view the information. We are used to...

...multiplexing is that you can ask how multiple proteins relate to one another in the same sample. For this strategy...are known to express your target or have been manipulated to overexpress it. For negative controls, use... The biggest difference between IF and ICC/IHC relates to the type of secondary antibody you use, so as...

...work does relate, make sure to include that material, but don’t force it. You can always relate to scientists... who will be listening.  Are they early, mid, or late career?  Will many non-scientists be present?  Always...

...speaker Paula Stephan did a fantastic job of articulating many of these problems and some solutions. The...provided as an explanation for why they are paid relatively low wages (Stephan estimates that postdocs are...that PIs no longer save grant money by paying relatively low postdoc salaries as opposed to Staff Scientists...

...controls and then moving on to controls for antibody-related applications. After reading, you will have a better...well-studied kinase known to prevent tau hyperphosphorylation. You could run brain samples from both groups... molecular weight shifts indicative of tau phosphorylation. By including the positive control, you will...

...the right decision. If I’d spent my senior year isolating pheromones from various C. elegans mutants by ...course, many types of teams. Some teams may be relatively permanent; some teams may only need to exist ...on the other side of the world, communication platforms like Slack make teamwork easier. Trust Ultimately...

...extract light from a single, thin plane within a relatively thicker three dimensional sample (see Figure ...technique should I use for my experiment? When I am contemplating which microscope to use for a new sample I always...optical sectioning (5). One such example is the lattice lightsheet that projects a number of thin cone-...

...sequence came from. Entering this information will populate a list of possible Entrez Gene matches to your...easier for other scientists to find information related to your plasmid. It is optional to include the ...correct Entrez Gene does not appear in the auto-populated list, try entering the gene's Official Full Name...

... first edition of our Antibodies 101 eBook! The latest addition to our eBook collection is a handy resource...start looking for answers.    We hope you find the latest addition to our eBook collection useful. And, as...

...package with Addgene mugs and some delicious hot chocolate for the lab. Here are some of our 2017 contest...Thank you to everyone who participated and congratulations to our 2017 #Deckthelab winners. We hope everyone...

...festive makeover today✂️❄️🦠thanks to the awesome templates by @CVRHutchinson @CVRinfo. Check them out: https...Mugford (@samtmugford) December 3, 2021   Congratulations to all our winners, and a huge thank you to...

...automated vial filling, accurate tube-labeling, quick plate pouring, and much more. We didn’t want to keep the...Tip” filter on the blog to keep up to date on the latest episode.  Have some suggestions for future tips...