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We narrowed to 1,010 results for: PLE

Showing: 661 - 680 of 1010 results

  1. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    ... and the complexity of effectively deploying them in the lab somewhat mirrors the complexity of understanding.... Addgene’s Viral Vector Service Besides being complex to use, viral vectors are also difficult both to...broadly useful vectors, a process that isn’t as simple as it seems. Take, for instance, the GCaMP-encoding...

  2. How to Be an Excellent Trainee

    Type
    Blog Post
    ...We think it’s so important that we have written plenty of articles on building successful mentor relationships...experience In research there is a saying that if the sample prep is poor, then the data will be poor. The same...you remember where the equipment, reagents, and samples are. Do: Ask your bench mentor if they have time...your mentor know what yours is! For instance, some people like to ask questions throughout training, while...

  3. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    ...tool is in such a small package. But don’t let the simple presentation deceive you; pooled libraries can ...visualization) that confirm diversity of the library sample, and any other information or scripts provided ...suffice…and the answer is no. Due to the size and complexity of pooled libraries, NGS is the best way to validate... plasmids didn’t amplify after your screen is completed!  Producing Your Lentiviral Library Once you've...

  4. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

    Type
    Blog Post
    ...only occur through editing by a APOBEC-Cas9n-UGI complex. So how exactly does this work? Figure 1:...DNA editing efficiency of APOBEC-Cas9 editisome complexes in real-time (St. Martin et al., 2019). To create...DNA editing efficiency of APOBEC-Cas9 editisome complexes. The eGFP reporters rely on the correction of ... single base editing by APOBEC-Cas9 editosome complexes. Scientific Reports 9: . https://doi.org/10.1038...

  5. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    ...the American Chemical Society (ACS) are just 2 examples of organizations that hold large, annual conferences...that did the same thing? Make an effort to meet people. Attend smaller talks within the bigger conference...professionals, with the added benefit that most people at the fair will probably be willing to accept ...country; sometimes several per month, giving you a plethora of opportunities to explore! Again, attending ...

  6. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ...become resistant somehow overtime. One fantastic example of intrinsic resistance would be Mycoplasma species...Figure 1): Over-prescription of antibiotics (for example, giving antibiotics for an infection that isn’t...treatment regiment might wipe out bacteria that are completely susceptible to a drug while a full course of ...likely to develop over time. It isn’t feasible to completely eliminate the development of resistance; it’s...

  7. Lab to Office Culture Shock

    Type
    Blog Post
    ...discovery can be hard since it’s frequently why many people get into science in the first place. The feeling...but for many graduate students and postdocs, the people you see in your bay for 8-12 hours a day become...worked at the bench for over 10 years, but when people ask me about my job, I have no qualms about saying...course, but you’ll inevitably have downtime and plenty of it. That’s not the case at an office job. I’...

  8. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    ...camera or tracking software. Locomotion is a highly complex process on the level of individual neurons and ...motivational and other aspects may be involved. For example, an animal moving around to explore the environment...the elevated plus maze, the light-dark box is a simple device comprised of a brightly lit (risky) and ...behavioral neuroscience to reveal insights into the complex relationship between genes, neurons, and behavior...

  9. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ...Amplified nucleotides are combined with Cas 13a in complex with crRNA targeting the sequence of interest and...sequences from a population (either enriching or depleting specific RNAs out of a pool of RNAs) or studying...cleavage system. LwaCas13a is also amenable to multiplexing; expressing 5 gRNAs simultaneously produces ... Featured Topic Page Learn about Anti-CRISPRs Multiplex Genome Editing with Cpf1 Resources on Addgene.org...

  10. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ...Feng Zhang's group previously packaged Cas9 and multiple gRNAs into separate AAV vectors, increasing overall... Wu X, Jiang W, Marraffini LA, Zhang F (2013) Multiplex Genome Engineering Using CRISPR/Cas Systems. Science...https://doi.org/10.1126/science.aaq0180  Fine EJ, Appleton CM, White DE, Brown MT, Deshmukh H, Kemp ML, Bao..., Nureki O (2014) Crystal Structure of Cas9 in Complex with Guide RNA and Target DNA. Cell 156:935–949...

  11. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ...are essential cell surface receptors involved in complex signaling pathways and the linkage of intra- and...proved challenging to study. Cells often express multiple integrins that bind the same ligand, making it...development. Integrin structure and conformational complexity There are 24 known mammalian integrins, all heterodimers...demonstrated that anti-integrin αVβ5.9 “essentially completely blocks all adhesion,” inhibiting the function...

  12. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ... into one of the loop regions of any G-protein-coupled receptor (GPCR) recognizing a specific ligand (...into an intracellular loop of various G-protein-coupled receptors. Image adapted from Ino et al. 2022, ...with different ITAM/SH2 reporter pairs allow multiplexed measurement of different RTKs. Although this ...to the range of concentrations expected in your samples. Higher affinity isn’t always better, especially...

  13. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type
    Blog Post
    ...or entire organism. Because each cell can have multiple copies of the transgene that will recombine independently... all cell types can be labeled, and that while multiple copies of the transgene can integrate into cells...dermis and identify that Langerhans cells are replenished through the proliferation of specific, and not... neurons over millimeter-long distances across multiple fields of view, whereas previous Brainbow technologies...

  14. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...plasmids with the piggyBac™ transposase itself, please read on to learn more about this exciting technology...also enables the excision of the transposon in a completely seamless manner, leaving no sequences or mutations...challenges still remain, such as making gene edits completely seamless (i.e., so no other genetic alterations...cytotoxic for proliferating cells and provides a simple tool for conditional cell ablation.,” Proc. Natl...

  15. Welcome to the Lab Bench! New Protocol Series Helps Trainees Get Started in the Lab

    Type
    Blog Post
    ...New to the lab? Or looking for ways to supplement your teaching? You’re in luck! Addgene’s Intro to the...vectors, but what we lacked were videos guiding completely new members of the lab or those taking introductory...techniques. It’s also great for teachers looking to supplement their curriculum. From the details about personal...

  16. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ...tens of thousands of biomolecules each containing multiple binding crevices that can nonspecifically soak...measureable FRET will be swamped out by a sea of uncoupled donors. The best method for targeting a fluorescent...NTA binds to the His tag on your protein and the coupled Cy fluorophore acts as the FRET acceptor. Binding... calibrated FRET distance measurements (for an example see Mahalingam et al., 2014).  Many thanks to...

  17. Overwhelmed? Take a Break With Our 5 Favorite Science Comics

    Type
    Blog Post
    ...lab? Addgene is after all a place that encourages people to take some time out every once in a while – just... stress in adolescents. It also put those young people in a better mood. Laughter itself can sometimes...and, of course, it’s a way to connect with other people. (We don't need evidence for that one, do we?) ...

  19. 5 Science Rap Videos & Their Creator: Tom McFadden

    Type
    Blog Post
    ...Watson & Crick - Science History Rap Battle A great example of science story telling following the unfortunate...importance in understanding biological history. 4. Please Don't Kill My Hive Beautifully filmed video about...science educator looking for creative ways to implement the next generation science standards and communicate...

  20. Bringing Renewable Energy to Addgene

    Type
    Blog Post
    ...sustainability effort we’ve implemented at Addgene. The kitchen and office areas provide multiple streams for composting... new programs, we're always striving to set an example of environmental stewardship.  Additional resources...
Showing: 661 - 680 of 1010 results