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We narrowed to 937 results for: Por

Showing: 661 - 680 of 937 results
  1. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    ...interacting proteins, which results in poor spatiotemporal resolution for in vivo work. APEX2, a faster...identify highly biotinylated background proteins reported by other research groups that performed BirA*-...
  2. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    ...Thesis (who has also blogged for us) offers a free report on her website called “Secrets to Success in Graduate...be helpful having a coach that can offer extra support for issues of diversity in science.  A great person...
  3. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    ... reactive ones. And make sure to check for any reported cross-reactivity when planning your experiment...Why Choosing the Right Secondary Is of Primary Importance. In J. Sturtevant (Ed.), PLoS ONE (Vol. 7, Issue...
  4. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    ...antibodies for IHC. Working with collaborators and support from the Brain Research Through Advancing Innovative...recombinant antibodies are a newer technology, it is important to show through qualitative data that they are...
  5. Hot Plasmids - October 2020

    Type
    Blog Post
    ...grown in high concentrations of IL-3 show a high proportion of blue fluorescence, indicating cell proliferation...they could not proliferate. Eastman et al. Cell Reports 2020. https://doi.org/10.1016/j.celrep.2020.107804...
  6. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    ...lines can be found in the Cell Catalog on our data portal. The plasmids should be functional in many different...and the donor plasmid into the cells we use electroporation, a technique that briefly destabilizes the ...
  7. Early Career Researcher Toolbox: Finding Relevant Papers

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    Blog Post
    ...about publications in the fields of research they support. Monitoring these types of accounts can help you...and figures using machine learning to extract important features such as influential citations, images...
  8. How-to: 5 Steps to a Great Panel Discussion

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    Blog Post
    ...so her ability to control the event is what is important. Choose 4 panelists (maybe 5) and no more. Too... members before a panel discussion and often incorporate them into the program. I have to admit to inviting...
  9. Production of Virus in Insect Versus Mammalian Cells

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    Blog Post
    ...of production, saving about a week of work. An important note is that this is only possible because baculoviruses... will come down to which differences are most important to you — virus type, cost, time, or reagents and...
  10. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    ... In our study, we used the TRIP (Thousands of Reporters Integrated in Parallel; Akhtar et al., 2013; Schep...Chromatin Position Effects Assayed by Thousands of Reporters Integrated in Parallel. Cell, 154(4), 914–927....
  11. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    ... a fluorescent signal. The signal increases proportionally to the amount of product produced each cycle...multiple starting DNA templates in one sample. It is important to note however, that multiplex PCR is more complicated...
Showing: 661 - 680 of 937 results