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...grown in high concentrations of IL-3 show a high proportion of blue fluorescence, indicating cell proliferation...they could not proliferate. Eastman et al. Cell Reports 2020. https://doi.org/10.1016/j.celrep.2020.107804...

... back to her church community which is really important to her. Addgenie Shreya Vedantam with our... although her focus is finance, it is just as important to bring love and fun to the office. Main takeaways...

...selection of guideRNAs. Of particular note, ABE8s reported in this work did not cause genome-wide, guide-...guide-independent off-target deamination, an important attribute for therapeutic application. ABE8s demonstrate...

...about publications in the fields of research they support. Monitoring these types of accounts can help you...and figures using machine learning to extract important features such as influential citations, images...

...Perturbation Platform, to produce their shRNA library to support functional investigations of the mammalian genome...together with expression of fluorescent or enzymatic reporters for cellular assays and animal imaging studies...

...lines can be found in the Cell Catalog on our data portal. The plasmids should be functional in many different...and the donor plasmid into the cells we use electroporation, a technique that briefly destabilizes the ...

...of AAAS, WCSJ or ECSJ. These meeting are THE opportunity to grow your network. Get to know and get known...or newspaper (here’s a partial list). A great opportunity is the AAAS Mass Media Fellowship, which places...

...introduce modifications at the DNA level in a temporally and locally defined manner. This allows scientists...injection Viral vectors allow a tight spatial and temporal control of gene expression manipulation. They ...

...so her ability to control the event is what is important. Choose 4 panelists (maybe 5) and no more. Too... members before a panel discussion and often incorporate them into the program. I have to admit to inviting...

...IgG standards in Google Sheets. Open exported CSV file. Select import. Choose file. Format as needed. Select...that sharing the full details of our protocols supports reproducibility and accelerates science. Here,...information is solely for transparency intended to support reproducibility in science. Last Update: June 14...intensity of the protein bands to the overall lane. Import the gel image into ImageJ. Select File . Select... each peak by selecting the center of the peak. Export results as a csv file. In the results table, select...

... a fluorescent signal. The signal increases proportionally to the amount of product produced each cycle...multiple starting DNA templates in one sample. It is important to note however, that multiplex PCR is more complicated...

...of production, saving about a week of work. An important note is that this is only possible because baculoviruses... will come down to which differences are most important to you — virus type, cost, time, or reagents and...

...cells that have randomly integrated all or large portions of the plasmid. Deliver your construct to your...marker, but cells with random recombination may incorporate the negative selection marker into the genome...

...area (the amount of agarose/DMEM should be proportionately increased). For some cells, add a drop of serum...antibiotics. Lastly, and perhaps obviously, it is important to have a functional assay in place to screen ...

... Golden Gate assemblies feature an inverse proportionality between the complexity of the assembly (number...The future of assembly DNA assembly methods are important tools for many areas of science, and researchers...

... purposes. We’re here to help with that! Four important factors to consider for any experiment are: ...Efficiency For cloning in particular, another important consideration is: The number of inserts ......

... In our study, we used the TRIP (Thousands of Reporters Integrated in Parallel; Akhtar et al., 2013; Schep...Chromatin Position Effects Assayed by Thousands of Reporters Integrated in Parallel. Cell, 154(4), 914–927....

...technique can be adapted to other transgenes. It’s important to note that the fluorescence level observed will...to insert cDNA constructs into this locus. One important question to ask is how the efficiency of PITCh-based...

... accidental release. Science relies on popular support, which in turn depends on widespread trust in scientists...environment, they must never be used without popular support2. An accidental release could delay real-world ...

...have that conversation out in the open is really important. Addgene: Is there anything else you’d like to...biology is massively underfunded considering how important it is. I would really like to reach all those ...