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We narrowed to 921 results for: Por

Showing: 661 - 680 of 921 results

  3. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    ... reactive ones. And make sure to check for any reported cross-reactivity when planning your experiment...Why Choosing the Right Secondary Is of Primary Importance. In J. Sturtevant (Ed.), PLoS ONE (Vol. 7, Issue...

  4. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    ...lines can be found in the Cell Catalog on our data portal. The plasmids should be functional in many different...and the donor plasmid into the cells we use electroporation, a technique that briefly destabilizes the ...

  5. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    ...so her ability to control the event is what is important. Choose 4 panelists (maybe 5) and no more. Too... members before a panel discussion and often incorporate them into the program. I have to admit to inviting...

  6. Protocol - How to Perform Sequence Analysis

    Type
    Protocol
    ...recommends that you sequence any portion of the plasmid that is important for your experiments. There are... Digest Introduction Sequence verification of important plasmid features (such as the gene/insert, fusion...sequences the plasmid to verify tags, mutations and a portion of the insert, but we do not sequence the entire...an example of a trace file from a high-quality portion of a sequencing reaction: Sequence near the beginning...unreliable. This is an example of a trace file from a portion of a sequencing reaction with high background: ...

  7. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    ... a fluorescent signal. The signal increases proportionally to the amount of product produced each cycle...multiple starting DNA templates in one sample. It is important to note however, that multiplex PCR is more complicated...

  10. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    ...area (the amount of agarose/DMEM should be proportionately increased). For some cells, add a drop of serum...antibiotics. Lastly, and perhaps obviously, it is important to have a functional assay in place to screen ...

  11. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    ...of production, saving about a week of work. An important note is that this is only possible because baculoviruses... will come down to which differences are most important to you — virus type, cost, time, or reagents and...

  12. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    ... In our study, we used the TRIP (Thousands of Reporters Integrated in Parallel; Akhtar et al., 2013; Schep...Chromatin Position Effects Assayed by Thousands of Reporters Integrated in Parallel. Cell, 154(4), 914–927....

  13. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    ...cells that have randomly integrated all or large portions of the plasmid. Deliver your construct to your...marker, but cells with random recombination may incorporate the negative selection marker into the genome...

  14. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ...technique can be adapted to other transgenes. It’s important to note that the fluorescence level observed will...to insert cDNA constructs into this locus. One important question to ask is how the efficiency of PITCh-based...

  18. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    ...channels both digitally and physically. Easy to incorporate visuals if needed. Traditional media formats ...assuming a high resource geographic location) Opportunity for real time engagement while allowing for recording...

  19. Great Results Start with Great Standard Curves

    Type
    Blog Post
    ...plotting the entire data set, plot only the linear portion. This allows you to use a simple linear regression... enough if your unknown lies within the linear portion of the graph. It does restrict the dynamic range...

  20. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    ... genetic engineering of a tag or fusion with a reporter enzyme. For multicolor staining, careful consideration...al. Before picking a secondary nanobody, it’s important to review a few characteristics of your primary...
Showing: 661 - 680 of 921 results