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... primer design or batch analysis. We wanted to relieve scientists of the burden of having to manage all...

...the oldest system reviewed here. The AID system relies on three components. First, the protein of interest...

...for Disease and Open Science: An Interview with Darrell Kotton Four Factors that Differentiate the Stem...

...four times in a 95% ethanol and dry ice bath to release the virus particles from the cells. Cell lysates...

... molecular viewing software.   In reality, this rarely happens. But if you ever have an opportunity to...

...the tag, selectable marker, and connector to be released (Fig. 1, Step 2-3). Since donor plasmids of the...

.... If the solution is too cold, it will clump prematurely, and not spread out onto the plate. We test the...

...expression plasmids. Cas9 guide RNA delivery does however rely on cloning into a vector or in vitro synthesis, ...

...-gRNA creates two DSBs in the PITCh plasmid to release the cassette, and an insertion-locus-specific gRNA...

... for which the underlying genetic cause is not entirely known. Identifying which genes are important for...

...antibodies conveniently allow for unlimited production, reliable expression, and easy distribution as DNA (Trimmer...

...sites from the 18,385 observed with REPAIRv1 to merely 20. Expanding RNA Editing Capabilities through ...

...of the growth factor IGF2 open reading frame to relax imprinting (Zhang et al., 2017). This upregulates...

... AAV questions every year on a range of issues related to AAV vectors, many from people trying to answer...

...maintaining the structure of your samples, the relationships between cellular components, and the accessibility...

...around. Finally, purified total nucleic acids are released by dipping the cellulose directly into a PCR buffer...

... can be cleaved by the bdNEDP1 protease, thus releasing purified nanobodies from the column. bdNEDP1 protease...

...X-linked retinitis pigmentosa, achromatopsia, and age-related macular degeneration are ongoing and some will ...

...CpG dinucleotides in their 6-mer sequences, are rarely found in the transcriptomes of these species and...

...set up, conducted and analysed with care to get reliable information. Although two-hybrid systems are a...