...furimazine by Promega’s Chemistry group is a good story (1). From a deep sea shrimp to a compact powerhouse...

...Symposium and #MeTooSTEM, academic bullies are hard to stop—they often have no boss or management structure ...

...., Butler, R., Bewersdorf, J., Rothman, J. E., Johnston, D. S., Schepartz, A., & Toomre, D. (2019). Labeling...

...distribution you are working with, graphing data with a histogram is sometimes sufficient to determine normality...

...move into science policy. Read broadly about the historical roots of science policy to connect to your why...

...Targeted amplicon sequencing doesn’t tell the whole story Figure 1: Schematic representations of the...

...experiments. Immune response dynamics are much better understood thanks to these technologies but many questions...

... then gate the B cell population and create a histogram of CD19-containing cells versus total cell number...

...and for good reason. For the first time in the history of neuroscience research we have a technology that...

...Illumination (SIM), Single Molecule Localization (PALM/STORM), or Reversible Saturable Optical Linear Fluorescence...

...from Nicole Gaudelli in David Liu’s lab in 2016. History of base transition editing Cytosine base editing...

... due to its condensed design. For both systems, custom spacers/gRNAs are easily inserted using BbsI and...

...accurate Check plasmid sequence for frameshifts and stop codons Weird background patterns (dots, lines...

...family of GFP proteins in the cephalochordate Branchiostoma floridae, named bfloGFPs. They went on to characterize...

...ng/µL stock into 900 µL PBS in a microfuge tube and vortex. 1 ng/µL : Add 450 µL of 2 ng/µL stock into ...multichannel pipette, add 100 µL of TMB Stop Solution directly to the wells to stop the reaction. Measure the absorbance...different incubation options available. Caution Use the Stop Solution in a well ventilated area and avoid breathing...tube and vortex. 0.5 ng/µL : Add 450 µL of 1 ng/µL stock into 450 µL PBS in a microfuge tube and vortex. .... 0.25 ng/µL : Add 450 µL of 0.5 ng/µL stock into 450 µL PBS in a microfuge tube and vortex. 0.125 ng/...ng/µL : Add 450 µL of 0.25 ng/µL stock into 450 µL PBS in a microfuge tube and vortex. 0 ng/µL : 450 µL...concentrations of HRP yield a greenish solution. Stop the reaction before any wells display a green product...

...sterile tubes. Store the tubes at -20 °C or -80 °C. After thawing, the solution can be stored at 4 °C for... the harvested media to a polypropylene storage tube and store at 4 °C between harvest. Centrifuge the... of heat-inactivated FBS and 5 mL of glutaGRO. Store at 4 °C. Pro-Tip Different brands and lots of FBS...months, discard the tube and thaw a new working stock. The optimal mass DNA:mass PEI ratio will need to... to batch variation when making the PEI working stock, therefore the ratio of μg DNA:μg PEI needs to be....45 μm PES filter. The viral supernatant can be stored at 4 °C for several hours but should be aliquoted...aliquoted and snap frozen in liquid nitrogen and stored at -80 °C as soon as possible to avoid loss of titer...

...multiple “stops” on the plunger, where you feel resistance while pressing down on the pipette. Stop at the...the first “stop.” The first stop expels enough air from the pipette for an accurate measurement in the ...way to the second stop to release the liquid. Pro-Tip Dispense until the first stop, wait, and then dispense...the next step. The second stop is meant to expel excess liquid from the tip once you’ve reached step 8. ... to its resting position. Wait for the liquid to stop flowing into the pipette tip. Liquids of various...dispense to the second stop to expel any residual liquid in the tip. Before releasing the plunger, lift...

... 2.5 μL Forward Primer (10 μM stock) 2.5 μL Reverse Primer (10 μM stock) 36.8 μL Sterile dH 2 O 0.2 μL... 2.5 μL Forward Primer (10 μM stock) 2.5 μL Reverse Primer (10 μM stock) 0.2 μL Taq DNA Polymerase (5 ...making a 1:10 dilution of the stock. For example, add 100µl of primer stock to 900µl of dH 2 O. Tips and...liquid, normally sterile dH 2 O. To make a 100uM stock of any primer, add a number of µl of dH 2 O equal...38.5nm, add 385µl of water. After making your 100uM stock, immediately make a working concentration of each...to supplement the amount supplied by the buffer stock, where it is known to form a gradient when frozen...

... A6279 Before Starting Warm the DNA and working stock of PEI-MAX to room temperature before use, and warm...sterile 0.22 µm filter. Prepare 10 mL aliquots and store the solution at -20 °C. 1 mg/mL PEI-MAX Add 1 g ... through a 0.22 µm filter. Prepare aliquots and store at -20 °C until use. BCD TFX 1000 mL BalanCD HEK293...mL 200 mM Glutagro Do not add selective reagents Store at 4 °C until use. We suggest preparing fresh solutions...500 mL BalanCD HEK293 Feed 20 mL 200 mM Glutagro Store at 4 °C until use. We suggest preparing fresh solutions... the second tube of 6 mL BCD TFX (for a 1 mg/mL stock solution of PEI-MAX, 450 µg = 450 µL). Cap the tube...our Recombinant Antibody Purification Protocol ). Store the supernatant at 4 °C until ready to use....

... plates) and store at -20 °C. Once a standard is thawed do not freeze it again but store at 4 °C and use...standard curve plasmid (2 x 10 9 stock made in step #1): Volume of 2 x 10 9 stock or previous dilution (μL) ...Water 4.7 μL 470 μL Procedure Prepare a plasmid stock of 2 x 10 9 molecules/μL to generate a standard ...79.8 = 1.25 μL Therefore we need to dilute 1.25 μL stock into 98.74 μL H 2 0 Pro-Tip Once a validated standard...Total dilution Dilution 1 (DNase step) 5 uL AAV stock 45 uL 10X 10X Dilution 2 5 uL Dil. 1 95 uL 20X 200X...