We narrowed to 728 results for: STO;
-
TypeBlog Post...laboratory research management consultant and coach in Boston, MA. He specializes in assisting research scientists...
-
Chemogenetics Guide
TypeGuide...phospholipase C, which releases intracellular calcium stores. There are currently three Gq DREADDs based on ... new window) Chen X, Choo H, Huang X-P, Yang X, Stone O, Roth BL, Jin J (2015). The First Structure–Activity...46. PMID: 25937170 (Link opens in a new window) Weston M, Kaserer T, Wu A, Mouravlev A, Carpenter JC, ... Stem Cell-Derived Neurons Repair Circuits and Restore Neural Function. Cell Stem Cell. 28:112-126.e6.... -
Is this the right place for me? 8 tactics for choosing a lab
TypeBlog Post...Symposium and #MeTooSTEM, academic bullies are hard to stop—they often have no boss or management structure ... -
Technologies Enabled by NanoLuc® Luciferase
TypeBlog Post...furimazine by Promega’s Chemistry group is a good story (1). From a deep sea shrimp to a compact powerhouse... -
Viral Vectors 101: AAV Variables That Matter
TypeBlog Post...Kügler, S. (2017). A MicroRNA124 Target Sequence Restores Astrocyte Specificity of gfaABC1D-Driven Transgene... -
Exploring Applications of the Bioluminescent HiBiT Tag
TypeBlog Post...protein activity. Bioluminescence detection is a cornerstone of modern cell-based assays and enables ultra-sensitive... -
Generating Mouse Models Using CRISPR/Cas9
TypeBlog Post...Humanization (25-kbp) Created Using Embryonic Stem Cell/Blastocyst and CRISPR/Zygote Injection Approaches. Sci Rep... -
PiggyBac-ing Through the Genome Editing Field
TypeBlog Post...re-cutting. Transposase removal of the cassette later restores the target sequence. This allows researchers to... -
Plasmids 101: Restriction Cloning
TypeBlog Post...to covalently link the backbone and insert and, PRESTO, you have a full plasmid ready to be used in your... -
Molecular Biology Reference
TypeGuide...bacterial cells that have been preserved as glycerol stocks. Function in many species — Plasmids can drive ...inoculating bacterial cultures . Antibiotic Recommended Stock Concentration Recommended Working Concentration ... acid. There are 20 amino acids plus a start and stop codon. Below you will find helpful resource tables..., UAC Valine Val V GUU, GUC, GUA, GUG Start AUG* Stop UAG (amber), UGA (opal), UAA (ochre) *AUG is the... -
Deep Dive: Statistical Tests (Comparisons)
TypeBlog Post...distribution you are working with, graphing data with a histogram is sometimes sufficient to determine normality... -
Finding Your Science Policy Path
TypeBlog Post...move into science policy. Read broadly about the historical roots of science policy to connect to your why... -
Sequencing Options for CRISPR Genotyping
TypeBlog Post...Targeted amplicon sequencing doesn’t tell the whole story Figure 1: Schematic representations of the... -
Antibodies 101: Flow Cytometry
TypeBlog Post... then gate the B cell population and create a histogram of CD19-containing cells versus total cell number... -
Live and Let Dye: Self-Labeling Protein Tags
TypeBlog Post...., Butler, R., Bewersdorf, J., Rothman, J. E., Johnston, D. S., Schepartz, A., & Toomre, D. (2019). Labeling... -
Which Fluorescence Microscopy Technique is Best for Me?
TypeBlog Post...Illumination (SIM), Single Molecule Localization (PALM/STORM), or Reversible Saturable Optical Linear Fluorescence... -
Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins
TypeBlog Post...experiments. Immune response dynamics are much better understood thanks to these technologies but many questions... -
A Primer on Optogenetics: Introduction and Opsin Delivery
TypeBlog Post...and for good reason. For the first time in the history of neuroscience research we have a technology that... -
Promoters
TypeGuide...general transcription factor proteins and histones can bind. Histones are proteins found in eukaryotic cells...cells that package DNA into nucleosomes. Histone binding prevents the initiation of transcription whereas... -
Molecular Cloning Techniques
TypeGuide...their recognition site allows for the creation of custom overhangs, which is not possible with traditional...have utilized this approach to create compatible custom overhangs that can then be efficiently assembled...yeast the fragments of DNA to be fused along with custom synthesized DNA oligos that span each junction....